Soil metagenome-derived 3-hydroxypalmitic acid methyl ester hydrolases suppress extracellular polysaccharide production in Ralstonia solanacearum

Lee, Myung Hwan; Khan, Raees; Wang, Tao; Choi, Kihyuck; Lee, Jae Woo; Hwang, Eul Chul; Lee, Seon-Woo

doi:10.1016/j.jbiotec.2018.01.023

Cited by 12 publications

(8 citation statements)

References 61 publications

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“…Interestingly, lipolytic enzymes from culture‐based sources, capable of the hydrolysis of 3‐OH PAME, suppressed EPS production in R. solanacearum strain AW1‐3 (Shinohara et al ., ). Furthermore, soil metagenome‐derived methyl ester hydrolases suppressed extracellular polysaccharide production in R. solanacearum strain GMI1000 (Lee et al ., ). These results demonstrate that reduced content of extracellular 3‐OH MAME/3‐OH PAME results in reduced activity of phc QS.…”

Section: Discussionmentioning

confidence: 97%

Contribution of a lectin, LecM, to the quorum sensing signalling pathway of Ralstonia solanacearum strain OE1‐1

Hayashi

Kai

Mori

et al. 2018

Molecular Plant Pathology

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Summary The soil‐borne bacterium Ralstonia solanacearum invades the roots and colonizes the intercellular spaces and then the xylem. The expression of lecM , encoding a lectin LecM, is induced by an OmpR family response regulator HrpG in R. solanacearum strain OE1‐1. LecM contributes to the attachment of strain OE1‐1 to the host cells of intercellular spaces. OE1‐1 produces methyl 3‐hydroxymyristate (3‐OH MAME) through a methyltransferase (PhcB) and extracellularly secretes the chemical as a quorum sensing (QS) signal, which activates QS. The expression of lecM is also induced by the PhcA virulence regulator functioning through QS, and the resulting LecM is implicated in the QS‐dependent production of major exopolysaccharide EPS I and the aggregation of OE1‐1 cells. To investigate the function of LecM in QS, we analysed the transcriptome of R. solanacearum strains generated by RNA sequencing technology. In the lecM mutant, the expression of positively QS‐regulated genes and negatively QS‐regulated genes was down‐regulated (by >90%) and up‐regulated (by ~60%), respectively. However, phcB and phcA in the lecM mutant were expressed at levels similar to those in strain OE1‐1. The lecM mutant produced significantly less ralfuranone and exhibited a significantly greater swimming motility, which were positively and negatively regulated by QS, respectively. In addition, the extracellular 3‐OH MAME content of the lecM mutant was significantly lower than that of OE1‐1. The application of 3‐OH MAME more strongly increased EPS I production in the phcB ‐deleted mutant and strain OE1‐1 than in the lecM mutant. Thus, the QS‐dependent production of LecM contributes to the QS signalling pathway.

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Section: Discussionmentioning

confidence: 97%

Contribution of a lectin, LecM, to the quorum sensing signalling pathway of Ralstonia solanacearum strain OE1‐1

Hayashi

Kai

Mori

et al. 2018

Molecular Plant Pathology

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“…In this study, crude enzymes with 3-OH PAME quenching activity were obtained from cell-free supernatant, which inhibited EPS production and cellulase activity of the R. solanacearum Rs-09-100, and all five strains of the biocontrol bacteria reduced the incidence of eggplant wilt, but the protein structures or the coding genes of the quenching enzymes were not identified ( Achari and Ramesh, 2015 ). Recently, some proteins including ELP86, ELP96, ELP104, and EstDL33 have been found from a soil metagenome library to degrade 3-OH PAME ( Lee et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…strain 0-0013 from soil using selective-enrichment culture method as a quorum quenching (QQ) agent that produces β -hydroxypalmitate methyl ester hydrolase ( β HPMEH) to degrade 3-OH-PAME, thereby suppressing the production of EPS and virulence of R. solanacearum ( Shinohara et al., 2007 ). A recent study identified novel esterases/lipases using soil metagenome sequencing that display various levels of hydrolytic activities towards 3-OH-PAME, and decrease the EPS production without affecting bacterial growth ( Lee et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas forestsoilum sp. nov. and P. tohonis biocontrol bacterial wilt by quenching 3-hydroxypalmitic acid methyl ester

Wang

Chen

et al. 2023

Front. Plant Sci.

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Bacterial wilt caused by Ralstonia solanacearum ranks the second top important bacterial plant disease worldwide. It is also the most important bacterial disease threatening the healthy development of Casuarina equisetifolia protection forest. 3-hydroxypalmitic acid methyl ester (3-OH PAME) functions as an important quorum sensing (QS) signal regulating the expression of virulence genes in R. solanacearum, and has been regarded as an ideal target for disease prevention and control. To screen native microorganisms capable of degrading 3-OH PAME, samples of C. equisetifolia branches and forest soil were collected and cultured in the medium containing 3-OH PAME as the sole carbon source. Bacteria with over 85% degradation rates of 3-OH PAME after 7-day incubation were further separated and purified. As a result, strain Q1-7 isolated from forest soil and strain Q4-3 isolated from C. equisetifolia branches were obtained and identified as Pseudomonas novel species Pseudomonas forestsoilum sp. nov. and P. tohonis, respectively, according to whole genome sequencing results. The degradation efficiencies of 3-OH PAME of strains Q1-7 and Q4-3 were 95.80% and 100.00% at 48 h, respectively. Both strains showed high esterase activities and inhibited R. solanacearum exopolysaccharide (EPS) and cellulase production. Application of strains Q1-7 and Q4-3 effectively protects C. equisetifolia, peanut and tomato plants from infection by R. solanacearum. Findings in this study provide potential resources for the prevention and control of bacterial wilt caused by R. solanacearum, as well as valuable materials for the identification of downstream quenching genes and the research and development of quenching enzymes for disease control.

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“…The hydrolysis of 3‐OH PAME by β‐hydroxypalmitate methyl ester hydrolase suppresses the phc QS‐dependent EPS production of R. solanacearum strain AW1‐3 (Shinohara et al, 2007). In addition, methyl ester hydrolases suppress the phc QS‐dependent EPS production of R. solanacearum GMI1000 (Lee et al, 2017). These data suggest that the functional PhcA levels may depend on the production and sensing of QS signals (Genin and Denny, 2012).…”

Section: Discussionmentioning

confidence: 99%

The putative sensor histidine kinase PhcK is required for the full expression of phcA encoding the global transcriptional regulator to drive the quorum‐sensing circuit of Ralstonia solanacearum strain OE1‐1

Senuma

Takemura

Hayashi

et al. 2020

Molecular Plant Pathology

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Soil metagenome-derived 3-hydroxypalmitic acid methyl ester hydrolases suppress extracellular polysaccharide production in Ralstonia solanacearum

Cited by 12 publications

References 61 publications

Contribution of a lectin, LecM, to the quorum sensing signalling pathway of Ralstonia solanacearum strain OE1‐1

Contribution of a lectin, LecM, to the quorum sensing signalling pathway of Ralstonia solanacearum strain OE1‐1

Pseudomonas forestsoilum sp. nov. and P. tohonis biocontrol bacterial wilt by quenching 3-hydroxypalmitic acid methyl ester

The putative sensor histidine kinase PhcK is required for the full expression of phcA encoding the global transcriptional regulator to drive the quorum‐sensing circuit of Ralstonia solanacearum strain OE1‐1

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