Soil management type differentially modulates the metabolomic profile of olive xylem sap

Sofo, Adriano; Fausto, Catia; Mininni, Alba Nicoletta; Dichio, Bartolomeo; Lucini, Luigi

doi:10.1016/j.plaphy.2019.04.036

Cited by 12 publications

(18 citation statements)

References 32 publications

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“…For instance, the metabolomic analyses disclosed a clear link between AMF and PGPR inoculation in pepper and basil respectively, increased crops yield and the accumulation of several PSC associated with crop defense to environmental stresses [ 75 , 138 ]. Similarly, the metabolome analysis revealed that olive growing in fields where no-tillage and crop residue cover were applied, shown higher PSC concentration in xylem sap [ 139 ]. Moreover, the proportion of essential amino acids and the contents of K, Mg, Na, Mn, Zn and tocopherol were higher in butternut squashes ( Cucurbita moschata ) growing in organic farming compared to conventional farming [ 140 ].…”

Section: Implications Of Soil Biodiversity For Nutrition and Food Safetymentioning

confidence: 99%

Improvement of Soil Microbial Diversity through Sustainable Agricultural Practices and Its Evaluation by -Omics Approaches: A Perspective for the Environment, Food Quality and Human Safety

2021

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Soil is one of the key elements for supporting life on Earth. It delivers multiple ecosystem services, which are provided by soil processes and functions performed by soil biodiversity. In particular, soil microbiome is one of the fundamental components in the sustainment of plant biomass production and plant health. Both targeted and untargeted management of soil microbial communities appear to be promising in the sustainable improvement of food crop yield, its nutritional quality and safety. –Omics approaches, which allow the assessment of microbial phylogenetic diversity and functional information, have increasingly been used in recent years to study changes in soil microbial diversity caused by agronomic practices and environmental factors. The application of these high-throughput technologies to the study of soil microbial diversity, plant health and the quality of derived raw materials will help strengthen the link between soil well-being, food quality, food safety and human health.

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Section: Implications Of Soil Biodiversity For Nutrition and Food Safetymentioning

confidence: 99%

Improvement of Soil Microbial Diversity through Sustainable Agricultural Practices and Its Evaluation by -Omics Approaches: A Perspective for the Environment, Food Quality and Human Safety

2021

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“…Our results were obtained using NMR and ICP-OES, that although is less sensitive than mass spectrometry, have been shown to provide powerful insights for highthroughput metabolomics application detecting a wide range of metabolites in an quantitative manner [64,65]. QTOF-MS provided to Sofo et al, [30] the wide discrimination of xylem sap secondary metabolism but only few primary metabolites including some amino acids (leucine, isoleucine, tyrosine, glutamate and arginine) and organic acids (fumaric and maleic acids) were detected in contrast to our results that involved the discrimination of the primary metabolism of xylem sap supplying five organic acids, 15 amino acids and five sugars, among other molecules. Furthermore, as a difference our work also provided information on the main mineral elements an inorganic anions present in the olive xylem sap.…”

Section: Discussionmentioning

confidence: 91%

“…However, most of the research has focused on the olive oil metabolome [e.g., [61][62][63]. To the best of our knowledge, only one work has studied the xylem sap composition based on the use of liquid chromatography (UHPLC) coupled to a hybrid quadrupoletime-of-flight mass spectrometer (QTOF-MS) [30]. Our results were obtained using NMR and ICP-OES, that although is less sensitive than mass spectrometry, have been shown to provide powerful insights for highthroughput metabolomics application detecting a wide range of metabolites in an quantitative manner [64,65].…”

Section: Discussionmentioning

confidence: 99%

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Metabolomic Characterization of Olive Xylem Sap Reveals Differences According to Plant Age and Genotype

Anguita-Maeso¹,

Haro²,

Montes-Borrego³

et al. 2021

Preprint

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Vascular pathogens are the causal agents of main diseases threatening the health and growth of olive crops worldwide. The use of endophytic microorganisms represents a challenging and promising strategy for management of vascular diseases in olive. Although current research has been focused on analyzing the structure and diversity of the endophytic microbial communities inhabiting the olive xylem, the characterization of this ecological niche has been overlooked and to date remain unexplored, despite that the characterization of the xylem sap composition is essential to unravel the nutritional requirements of xylem-limited microorganisms. In this study, branches from plantlets and adult olive trees of cultivars ‘Picual’ and ‘Arbequina' were selected to characterize the chemical composition of olive xylem sap extracted using a Scholander pressure chamber. Metabolome and ionome analyses of xylem sap were performed by proton nuclear magnetic resonance (NMR) spectroscopy-based and by inductively coupled plasma with optical emission spectroscopy (ICP-OES), respectively. Olive xylem sap metabolites included a higher relative percentage of sugars (54.35%), followed by alcohols (28.85%), amino acids (8.01%), organic acids (7.68%) and osmolytes (1.12%). Within each of these groups, the main metabolites in the olive xylem sap were mannitol, ethanol, glutamine, acetate and trigonelline, whereas K and Cl- were the main element and inorganic anion, respectively. Metabolomic profile varied when comparing olive plant age and genotype. The levels of glucose, fructose, sucrose and mannitol, choline, B and PO43 were significantly higher in adult trees than in plantlets for both olive genotypes, whereas NO3- and Rb content showed the opposite behavior. On the other hand, levels of aspartate, phenylalanine and Na were significantly higher in ‘Picual’ than in ‘Arbequina’ whereas Fe showed the opposite behavior but only for adult trees. Non-supervised hierarchical clustering analysis separated xylem sap composition firstly according to the plant age and then by the olive cultivar. Supervised PLS-DA analysis revealed that B, ethanol, Fe, Fructose, glucose, mannitol, sucrose and Sr were the most significative compounds discriminating adult trees from plantlets, whereas asparagine, aspartate, glutamate and phenylalanine or aspartate, arginine, ethanol and Sr were the most contributory compounds in the discrimination of both olive genotypes for adult trees or plantlets, respectively. Knowledge of the chemical composition of xylem sap will lead to a better understanding of the complex nutritional requirements of olive xylem-inhabiting microorganisms, including its vascular pathogens, and would allow the design of artificial growing media to improve culturing the olive microbiome.

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“…In the secondary metabolites class were included a wide range of chemically diverse and functionally important compounds, like phenolics, alkaloids, and terpenes. Broadly, these secondary metabolites are often produced as an adaptive response to specific stress [ 39 , 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Profile, Mineral Content, and Bioactive Compounds in Leaves of Seed-Propagated Artichoke Hybrid Cultivars

et al. 2020

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The globe artichoke (Cynara cardunculus L. subsp. Scolymus (L.) Hegi) is a multi-year species rich in various classes of phytochemicals with known nutritional and pharmacological properties, such as polyphenols, sesquiterpene lactones, and terpenoids. Over the last decade, hybrids cultivars are transforming the artichoke market for their higher uniformity and stability over the traditional landraces, further increasing the potential of the artichoke as a source of commercial extracts and bioactive molecules. Our aim was to investigate the mineral and phytochemical profiles of leaves from seven seed-propagated hybrids by using an untargeted metabolomic approach based on ultra-high-pressure liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry. Metabolomics identified several compounds in the tested varieties, namely 98 polyphenols, 123 sesquiterpene lactones, and 221 other metabolites. The phenolic content ranged from 3.01 mg Eq./g fw (for ‘Opera’) to 4.71 mg Eq./g fw (for ‘Opal’). Sesquiterpene lactones were, on average, 2.11 mg Eq./g fw. Multivariate statistics (HCA, PCA and OPLS-DA) highlighted the main metabolomics differences among cultivars, which weakly correlated with their agronomic classification. The seven cultivars showed distinctive metabolomics profiles, with ‘Opal’ and ‘Istar’ being the most valuable hybrids. The 3-hydroxyphenyl-valeric acid (a medium-chain fatty acid) and the 6-Gingesulfonic acid (a methoxyphenol) were the most discriminant markers. Our findings illustrated the quantitative and qualitative variation of several classes of phytochemicals in seed-propagated artichoke cultivars and allowed identifying distinctive metabolic signatures for both phenolic compounds and sesquiterpene lactones. This work supports the exploitation of the artichoke leaves from hybrid cultivars as a rich source of bioactive phytochemicals.

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Soil management type differentially modulates the metabolomic profile of olive xylem sap

Cited by 12 publications

References 32 publications

Improvement of Soil Microbial Diversity through Sustainable Agricultural Practices and Its Evaluation by -Omics Approaches: A Perspective for the Environment, Food Quality and Human Safety

Improvement of Soil Microbial Diversity through Sustainable Agricultural Practices and Its Evaluation by -Omics Approaches: A Perspective for the Environment, Food Quality and Human Safety

Metabolomic Characterization of Olive Xylem Sap Reveals Differences According to Plant Age and Genotype

Phytochemical Profile, Mineral Content, and Bioactive Compounds in Leaves of Seed-Propagated Artichoke Hybrid Cultivars

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