Greenhouse experiments were conducted to study the effects of various levels of N, K, Mg, Ca, P, and S on the concentration of amino acids in soybean plant hydrolyzates. Deficiency and excess amounts of N increased the amino acid contents (µ mole/g dry tissue) in the tops, while in the roots the amino acid contents increased as N concentration increased in the root media. Aspartic acid, arginine, and histidine contents of tops were affected most by N treatments. Although the percent N of potassium deficient soybean tops was higher than those plants receiving potassium, their total amino acid contents were of the same order of magnitude. A negative correlation was found between the aspartic acid content of the soybean tops and the potassium concentration of the nutrient media. Similar results were obtained for plants grown at various Mg levels. Calcium levels had little effect on the concentration of amino acids in soybean plants. Increased arginine and aspartic acid and decreased glutamic, proline, glycine, alanine, valine, and leucine contents were noted with P deficient plants. The total amino acid content was highest at 30 ppm P level. A positive correlation was noted between the total amino acid content of tops and the sulfur concentration of the root media. In general, high amino acid contents were found in plants grown under conditions where the macroad micro‐nutrients were present in sufficient amounts and proper balance.