Soil extracellular enzyme activities and the abundance of nitrogen-cycling functional genes responded more to N addition than P addition in an Inner Mongolian meadow steppe

Xiao, Hu; He, Yang; Zhao, Mengli; Monaco, Thomas A.; Rong, Yiming; Huang, Ding; Song, Qian; Zhao, Kun; Wang, Deping

doi:10.1016/j.scitotenv.2020.143541

Cited by 39 publications

(23 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Soil enzymes are mainly produced by soil microorganisms to decompose organic matter into available nutrients for its assimilation and soil enzymes activities could be an indicator of soil microbial nutrient demand (Fan et al, 2018). N addition significantly increased the invertase activity (Figure 3), as found in other studies (Xiao et al, 2021). This result supports the resource allocation theory, which states that N addition stimulates the activities of C-and P-cycling enzymes, but inhibits N-cycling enzyme activities (Allison and Vitousek, 2005).…”

Section: Effects Of Nitrogen and Phosphorus Addition On Soil Enzyme A...supporting

confidence: 90%

Differentiate Responses of Soil Microbial Community and Enzyme Activities to Nitrogen and Phosphorus Addition Rates in an Alpine Meadow

Wang

2022

Front. Plant Sci.

View full text Add to dashboard Cite

Nitrogen (N) and phosphorus (P) are the dominant limiting nutrients in alpine meadows, but it is relatively unclear how they affect the soil microbial community and whether their effects are rate dependent. Here, N and P addition rates (0, 10, 20, and 30 g m–2 year–1) were evaluated in an alpine meadow and variables related to plants and soils were measured to determine the processes affecting soil microbial community and enzyme activities. Our results showed that soil microbial biomass, including bacteria, fungi, gramme-negative bacteria, and actinomycetes, decreased along with N addition rates, but they first decreased at low P addition rates (10 g m–2 year–1) and then significantly increased at high P addition rates (30 g m–2 year–1). Both the N and P addition stimulated soil invertase activity, while urease and phosphatase activities were inhibited at low N addition rate and then increased at high N addition rate. P addition generally inhibited peroxidase and urease activities, but increased phosphatase activity. N addition decreased soil pH and, thus, inhibited soil microbial microorganisms, while P addition effects were unimodal with addition rates, achieved through altering sedge, and available P in the soil. In conclusion, our studies indicated that soil microbial communities and enzyme activities are sensitive to short-term N and P addition and are also significantly influenced by their addition rates.

show abstract

Section: Effects Of Nitrogen and Phosphorus Addition On Soil Enzyme A...supporting

confidence: 90%

Differentiate Responses of Soil Microbial Community and Enzyme Activities to Nitrogen and Phosphorus Addition Rates in an Alpine Meadow

Wang

2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…The grassland was replanted with Bromus inermis in 1997 and mowed for hay regularly until the grazing experiment began in 2015. The plant community at the site was dominated by the plant species Leymus chinensis , Bromus inermis , Potentilla bifurca , Carex pediformis and Carex duriuscula [ 29 ]. In this experiment, moderate continuous grazing (CG), grazing exclusion (CK), and mowing treatment (MG) were used ( Figure 6 c).…”

Section: Methodsmentioning

confidence: 99%

“…The composite soil samples were divided into two portions by weight; one portion was naturally dried in the shade, and the other portion was stored at −20 °C before further analyses. Soil NH 4 + -N and NO 3 − -N concentrations were measured using a continuous flow auto-analyzer (Auto-Analyses 3, Seal Analytical, Norderstedt, Germany) [ 29 ]. Soil samples at the same time were taken to the laboratory and dried at 105 °C for 48 h to a constant weight to determine soil moisture content.…”

Section: Methodsmentioning

confidence: 99%

“…and Potentilla bifurca Linn. [ 29 ]. The main objectives of this study were (1) to examine whether plant C/N ratios of different dominant species would show different responses to grazing and mowing; (2) to investigate the process by which soil water and nitrogen availability affect the shoot C/N and root C/N under different grassland utilization.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Grazing and Mowing Affect the Carbon-to-Nitrogen Ratio of Plants by Changing the Soil Available Nitrogen Content and Soil Moisture on the Meadow Steppe, China

Wang

Zhang

et al. 2022

Plants

View full text Add to dashboard Cite

Common grassland management practices affect plant and soil element stoichiometry, but the primary environmental factors driving variation in plant C/N ratios for different species in different types of grassland management remain poorly understood. We examined the three dominant C/N stoichiometric responses of plants to different land uses (moderate grazing and mowing) in the temperate meadow steppe of northern China. Our results showed that the responses of the C/N ratio of dominant plants differed according to the management practice. The relative abundance of N in plant tissues increased due to increased soil NO3−, with a consequent decrease in plant C: N in the shoots of Leymus chinensis, but the C/N ratio and nitrogen concentration in the shoots of Bromus inermis and Potentilla bifurca were relatively stable under short-term moderate grazing management. Mowing reduced the concentration of soil NH4+, thus reducing the nitrogen concentration of the roots, resulting in a decrease in the root C/N ratio of Potentilla bifurca. Structural equation model (SEM) showed that the root C/N ratio was affected by both root N and soil inorganic N, while shoot C/N ratio was only affected by the soil inorganic N. Our findings provide a mechanistic understanding of the responses of plant C/N ratio to land use change. The species-level responses of plant stoichiometry to human-managed grasslands deserve more attention.

show abstract

“…Such changes have been attributed to soil microbial processes associated to soil organic matter and to root exudates to mobilize nutrients held in soil organic matter (Keiluweit et al 2015). N additions can also have effects on soil microbiota and on P mineralization processes by altering extracellular enzyme activities and by promoting soil C mobilization (Xiao et al 2021). Short term quantification of P changes in organic P generally are difficult to measure in the laboratory studies due to the rapid sorption of released P (Bünemann 2015).…”

Section: Introductionmentioning

confidence: 99%

Long-term changes in organic and inorganic phosphorus compounds as affected by long-term synthetic fertilisers and pig manure in arable soils

Xue

Wen²,

Zhang

et al. 2022

Plant Soil

View full text Add to dashboard Cite

was lower than on adding manure+superphosphate. Other monoester P compounds, typically related to microbial biomass and processes were highest on adding pig manure+urea. When adding superphosphate+urea+pig manure a reduction of these P compounds was observed as well as the highest accumulation of inositol hexakisphosphate (IHP). Synthetic fertilisers increased Olsen-P, orthophosphate and other monoester and total IHP and largely decreased soil pH. The addition of pig manure greatly increased the stereoisomers of IHP (myo-IHP, scyllo-IHP, neo-IHP, and D-chiro-IHP) and the concentration of orthophosphate diesters. D-chiro-IHP was not found in unfertilised soils while neo-IHP was not detected in synthetically fertilised soils. Conclusions P compounds related to microbial processes, such as other monoester, increased when adding pig manure. Additions of superphosphate on top of manures reduced other monoester, while they increased on adding synthetic fertilisers alone.

show abstract

Soil extracellular enzyme activities and the abundance of nitrogen-cycling functional genes responded more to N addition than P addition in an Inner Mongolian meadow steppe

Cited by 39 publications

References 59 publications

Differentiate Responses of Soil Microbial Community and Enzyme Activities to Nitrogen and Phosphorus Addition Rates in an Alpine Meadow

Differentiate Responses of Soil Microbial Community and Enzyme Activities to Nitrogen and Phosphorus Addition Rates in an Alpine Meadow

Grazing and Mowing Affect the Carbon-to-Nitrogen Ratio of Plants by Changing the Soil Available Nitrogen Content and Soil Moisture on the Meadow Steppe, China

Long-term changes in organic and inorganic phosphorus compounds as affected by long-term synthetic fertilisers and pig manure in arable soils

Contact Info

Product

Resources

About