BackgroundThe sharp decline in near-natural areas worldwide is undisputed, but the consequences of this decline, apart from the loss of biodiversity, cannot be fully assessed. Biotic components of a landscape are usually more easily assessed than the abiotic components, since biotic components are often more easily detectable. A forest of (semi)natural stocking was selected in the northeastern part of Brandenburg (northeast Germany) to check whether it can serve as reference site for near-natural conditions or not. Analyses of archival sources and historic maps as well as field investigations were combined to reconstruct the dynamics of vegetation and soil as far back in time as possible.ResultsPalynological data from nearby sites provide evidence that the investigated area has been forested for several thousands of years and has hardly been structurally influenced by humans in the last 450 years. This evidence together with historical maps of tree species composition allows us to infer that the specific forest has been very close to a natural state for at least 250 years. Soil investigations support this conclusion, since a soil inventory, field studies on two catenas and corings at selected depressions rarely show signs of anthropogenic erosion and related colluviation. Parts of the area were cleared in prehistory, but near-natural soils have been preserved in other parts. ConclusionsThe area with these undisturbed parts is regarded as an ideal reference site. With this study, we show that using a multi-source approach it is possible to find potential reference sites and that such an approach is applicable in other regions.