Soil copper uptake by land snails: A semi-field experiment with juvenile Cantareus aspersus snails

Drăghici, George Andrei; Dehelean, Cristina; Pînzaru, Iulia; Bordean, D. M.; Borozan, Aurica Breica; Tsatsakis, Aristidis; Kovatsi, Leda; Nica, Dragoş

doi:10.1016/j.etap.2019.103243

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…It is a well-known fact that soils act as the major sink for heavy metals (hereinafter referred to as metals) that are derived from both natural and anthropogenic sources [1][2][3][4]. Given their persistence and non-biodegradability, these elements easily accumulate in soils and are then further transferred along terrestrial food chains to the upper trophic levels (primary producers, herbivores, and predators) [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…In this context, this study aimed at providing environmental scientists with a multifaceted perspective on Zn, Cu, Fe, Mn, and Pb occurring in soils at a high-profile environmental hot spot in Europe by (1) determining their total levels and pollution degrees in soils impacted to different extents by anthropogenic activities; (2) evaluating metal soilto-plant transfer in selected cultivated and non-cultivated plant species; (3) and assessing the non-carcinogenic risk related to consumption of vegetables grown in unpolluted soils from the Bor area. We used flame atomic absorption spectrometry (FAAS) to quantify Zn, Cu, Fe, Mn, and Pb concentrations in soils at four sites and in aboveground parts of four spontaneous plant species (dandelion, nettle, coltsfoot, and creeping buttercup) and edible parts of eight vegetables routinely included in the human diet (onion bulbs, garlic bulbs, carrot roots, parsley roots, celery roots, potato tubers, dill leaves, and sorrel leaves).…”

Section: Introductionmentioning

confidence: 99%

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Potential Ecological and Human Health Risks of Heavy Metals in Soils in Selected Copper Mining Areas—A Case Study: The Bor Area

Filimon

Caraba

Popescu

et al. 2021

IJERPH

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Surface soil samples were collected near the Open Pit Bor (S1) and Open Pit Cerovo (S2), a grassland along the Borska Reka River (S3) and an unpolluted garden near Slatina village (reference site). Spontaneous plants (dandelion, nettle, coltsfoot, and creeping buttercup) and vegetables (onion, garlic, carrot, parsley, celery, potatoes, dill, and sorrel) were obtained from the former three sites and the reference site, respectively. The samples were analyzed for Zn, Cu, Fe, Mn, and Pb via FAAS. Pollution indices indicated low-to-moderate soil contamination at sites S1, S2, and S3. Cu was the main contaminant of environmental concern, being above the maximum admitted concentration at site S1. Metal levels in spontaneous plants were below phytotoxic levels. Cu content of leafy vegetables and celery roots and Pb content of most vegetables were not safe for human consumption. Metal concentrations tended to be significantly lower in plants than in soils, with only Cu occurring at significantly elevated levels in celery roots and sorrel leaves. Non-carcinogenic risk assessment showed that consumption of carrot roots and especially celery roots grown on unpolluted soils from the Bor area might pose long-term health risks for females and males, with the main contributors being Cu and Fe.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential Ecological and Human Health Risks of Heavy Metals in Soils in Selected Copper Mining Areas—A Case Study: The Bor Area

Filimon

Caraba

Popescu

et al. 2021

IJERPH

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“…14 In addition, the accumulation of copper in human body contribute to atherosclerosis and rapid cell aging posing a huge concern to public health. [15][16][17] Furthermore, excretion of copper from livestock causes soil deterioration and water pollution which can negatively affect the growth rate of plants [18][19][20][21] and pose ecological concerns. 22,23 Reduction of human exposure to copper is one of the main objectives of hygienists and public authorities.…”

Section: Introductionmentioning

confidence: 99%

Development of two highly sensitive and selective sensor-assisted fluorescence immunoassays for trace determination of copper residues in food samples

Darwish,

Wang,

Darling

et al. 2023

RSC Adv.

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“…In addition, the accumulation of copper in the human body contributes to atherosclerosis and rapid cell aging, posing a huge concern to public health [15][16][17]. Furthermore, excretion of copper from livestock causes soil deterioration and water pollution, which can negatively affect the growth rate of plants [18][19][20][21] and pose ecological concerns [22,23].…”

Section: Introductionmentioning

confidence: 99%

Development and Comparative Evaluation of Two Highly Sensitive Immunosensor Platforms for Trace Determination of Copper Ions in Drinking Water Using a Monoclonal Antibody Specific to Copper-EDTA Complex

Darwish,

Wang,

Darling

2023

Molecules

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This study describes the development of two highly sensitive immunosensor platforms for the trace determination of copper ions, Cu(II), in drinking water. These platforms were a microwell-based enzyme-linked immunosorbent assay (ELISA) and a kinetic exclusion assay (KinExA) with a KinExATM 3200 immunosensor. Both ELISA and KinExA were developed utilizing the same antibody and coating reagent. The antibody was a mouse monoclonal antibody, designated as 8D66, that specifically recognized Cu(II)-ethylenediamine tetraacetic acid complex (Cu(II)-EDTA) but did not recognize Cu(II)-free EDTA. The 8D66 monoclonal antibody was generated by the fusion of spleen cells of an immunized BALB/c mouse with SP2/0-Ag14 myeloma cells. The immunogen was a protein conjugate of Cu(II)-EDTA with keyhole limpet hemocyanin protein. The coating reagent was Cu(II)-EDTA covalently linked to bovine serum albumin protein (Cu(II)-EDTA-BSA). Both assays involved the competitive binding reaction between Cu(II)-EDTA complexes, formed in the sample solution, and Cu(II)-EDTA-BSA conjugate which has been immobilized onto ELISA plates (in ELISA) or polymethylmethacrylate beads (in KinExA) for a limited quantity of binding sites of the 8D66 antibody. In ELISA, color signals were generated by a peroxidase-labeled secondary antibody and 3,3′,5,5′-tetramethylbenzidine substrate. In KinExA, a fluorescein isothiocyanate-labeled secondary antibody was used to generate KinExAgram (trend-line fluorescence responses vs. time). The conditions of both ELISA and KinExA were investigated, and the optimum procedures were established. Both ELISA and KinExA were validated, and all validation parameters were acceptable. Many different metal ions that are commonly encountered in drinking water did not interfere with the Cu(II) analysis by both ELISA and KinExA. Both assays were applied to the determination of Cu(II) in drinking water with satisfactory accuracy and precision. Both assays were compared favorably with inductively coupled plasma atomic emission spectroscopy in terms of their abilities to accurately and precisely determine Cu(II) in drinking water samples. A comparative evaluation of ELISA and KinExA revealed that KinExA had a higher sensitivity and better precision than ELISA, whereas both assays had comparable accuracy. Both ELISA and KinExA were superior to the existing atomic spectrometric methods for Cu(II) in terms of sensitivity, convenience, and analysis throughputs. The proposed ELISA and KinExA are anticipated to effectively contribute to assessing Cu(II) concentrations and control the exposure of humans to its potential toxicities.

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Soil copper uptake by land snails: A semi-field experiment with juvenile Cantareus aspersus snails

Cited by 4 publications

References 39 publications

Potential Ecological and Human Health Risks of Heavy Metals in Soils in Selected Copper Mining Areas—A Case Study: The Bor Area

Potential Ecological and Human Health Risks of Heavy Metals in Soils in Selected Copper Mining Areas—A Case Study: The Bor Area

Development of two highly sensitive and selective sensor-assisted fluorescence immunoassays for trace determination of copper residues in food samples

Development and Comparative Evaluation of Two Highly Sensitive Immunosensor Platforms for Trace Determination of Copper Ions in Drinking Water Using a Monoclonal Antibody Specific to Copper-EDTA Complex

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