The challenge of agricultural sector is to reduce greenhouse gas (GHG) emissions while increasing food, fiber and energy production without jeopardizing environmental integrity. In the Andean zone of Colombia, there is a growing need to develop GHG mitigation techniques associated with milk production. The present study focuses on GHG emissions and potential sinks associated with milk production scenarios in the Andean zone of Colombia. The scenarios considered were as follows: conventional agriculture of Pennisetum clandestinum in rotation with potatoes (PRP), improved pastures of Lolium multiflorum (IP) and silvopastoral system of P. clandestinum in association with Acacia decurrens and Trifolium repens (SPS). Based on the IPCC (Guidelines for national greenhouse gas inventories. The intergovernmental panel on climate change, Institute for Global Environmental Strategies, Kanagawa, 2006. http://www.ipcc-nggip.iges.or.jp/ support/Primer_2006GLs.pdf) methodologies, the annual GHG emissions considering a 6-year production cycle included agricultural sources and gasoline consumption related to the most important agricultural phases in the field, and a potential for soil carbon accumulation and biomass carbon fixation in all the studied scenarios. The lowest GHG emissions were estimated in PRP scenario (3684 kg CO 2-eq ha-1 year-1), which also presented additional emissions because of soil carbon losses beyond the lower milk productivity. Highest GHG emissions were observed in IP scenario (7711 kg CO 2-eq ha-1 year-1), which exhibited the highest milk productivity and a considerable potential for soil carbon accumulation that could help to offset its emissions. Nevertheless, SPS scenario,