Soil-Borne Legacies of Disease in Arabidopsis thaliana

Vismans, Gilles; Spooren, Jelle; Pieterse, Corné M. J.; Bakker, Peter A. H. M.; Berendsen, Roeland L.

doi:10.1007/978-1-0716-1040-4_17

Cited by 4 publications

(6 citation statements)

References 17 publications

(22 reference statements)

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“…In this study, natural soil collected from the Reijerscamp nature reserve in the Netherlands was used 12 . The SBL setup was performed as previously described in detail (Methods S1) 29 . Briefly, approximately 30 Arabidopsis seeds were sown on 60-ml pots filled with soil and placed in a climate-controlled plant growth chamber at 21 °C, 70% relative humidity, 10-h light/14-h dark cycles.…”

Section: Methodsmentioning

confidence: 99%

Coumarin biosynthesis genes are required after foliar pathogen infection for the creation of a microbial soil-borne legacy that primes plants for SA-dependent defenses

Vismans

Bentum

Spooren

et al. 2022

Sci Rep

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Plants deposit photosynthetically-fixed carbon in the rhizosphere, the thin soil layer directly around the root, thereby creating a hospitable environment for microbes. To manage the inhabitants of this nutrient-rich environment, plant roots exude and dynamically adjust microbe-attracting and -repelling compounds to stimulate specific members of the microbiome. Previously, we demonstrated that foliar infection of Arabidopsis thaliana by the biotrophic downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa) leads to a disease-induced modification of the rhizosphere microbiome. Soil conditioned with Hpa-infected plants provided enhanced protection against foliar downy mildew infection in a subsequent population of plants, a phenomenon dubbed the soil-borne legacy (SBL). Here, we show that for the creation of the SBL, plant-produced coumarins play a prominent role as coumarin-deficient myb72 and f6’h1 mutants were defective in creating a Hpa-induced SBL. Root exudation profiles changed significantly in Col-0 upon foliar Hpa infection, and this was accompanied by a compositional shift in the root microbiome that was significantly different from microbial shifts occurring on roots of Hpa-infected coumarin-deficient mutants. Our data further show that the Hpa-induced SBL primes Col-0 plants growing in SBL-conditioned soil for salicylic acid (SA)-dependent defenses. The SA-signaling mutants sid2 and npr1 were unresponsive to the Hpa-induced SBL, suggesting that the protective effect of the Hpa-induced shift in the root microbiome results from an induced systemic resistance that requires SA-signaling in the plant.

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Section: Methodsmentioning

confidence: 99%

Coumarin biosynthesis genes are required after foliar pathogen infection for the creation of a microbial soil-borne legacy that primes plants for SA-dependent defenses

Vismans

Bentum

Spooren

et al. 2022

Sci Rep

Self Cite

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“…Especially, jasmonic acid pathway is also known to be activated during plant response to stress [81] and is supposed to be involved in the filtration of microbiota components [82]. When the protective effect against a pathogen is extended to the next generation, this process is called "the soil born legacy" [83,84].…”

Section: From Transient Dysbiotic State To Plant Resilience: the Cry ...mentioning

confidence: 99%

Plant microbiota dysbiosis and the Anna Karenina Principle

Arnault

Mony

Vandenkoornhuyse

2023

Trends in Plant Science

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“…One day prior to sowing, the soil was watered in a 1:10 v/w ratio. Pots with a volume of 60 mL were filled with 120 g of soil (+/- 2.5 g), placed in 60-mm Petri dishes and the soil surface was covered by a circular cut-out of plastic micro pipette tip holders (Greiner Bio-One, 0.5-10 μL, Item No: 771280) to prevent growth of algae and to ensure that seeds are equally distributed during sowing 54 . Pots were stored at 4°C overnight before sowing.…”

Section: Methodsmentioning

confidence: 99%

“…Plants were airdried for 2 h, covered with transparent lids to ensure high humidity and incubated (21°C, 10 h light/14 h dark, light intensity 100 μmol m -2 s -1 ). Seven days post inoculation of this conditioning population of plants, shoots were cut off and a new population of Col-0 plants (response population) was sown directly on top of the soil after which the experimental cycle was repeated 54 . For both the conditioning and response population of plants, Hpa and gnoHpa sporulation was quantified as described above.…”

Section: Methodsmentioning

confidence: 99%

Congruent downy mildew-associated microbiomes reduce plant disease and function as transferable resistobiomes

Goossens

Spooren

Baremans

et al. 2023

Preprint

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Root-associated microbiota can protect plants against severe disease outbreaks. In the model-plant Arabidopsis thaliana, leaf infection with the obligate downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa) results in a shift in the root exudation profile, therewith promoting the growth of a selective root microbiome that induces a systemic resistance against Hpa in the above-ground plant parts. Here we show that, additionally, a conserved subcommunity of the recruited soil microbiota becomes part of a pathogen-associated microbiome in the phyllosphere that is vertically transmitted with the spores of the pathogen to consecutively infected host plants. This subcommunity of Hpa-associated microbiota (HAM) limits pathogen infection and is therefore coined a resistobiome. The HAM resistobiome consists of a small number of bacterial species and was first found in our routinely maintained laboratory cultures of independent Hpa strains. When co-inoculated with Hpa spores, the HAM rapidly dominates the phyllosphere of infected plants, negatively impacting Hpa spore formation. Remarkably, isogenic bacterial isolates of the abundantly-present HAM species were also found in strictly separated Hpa cultures across Europe, and even in early published genomes of this obligate biotroph. Our results highlight that pathogen-infected plants can recruit protective microbiota via their roots to the shoots where they become part of a pathogen-associated resistobiome that helps the plant to fight pathogen infection. Understanding the mechanisms by which pathogen-associated resistobiomes are formed will enable the development of microbiome-assisted crop varieties that rely less on chemical crop protection.

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Soil-Borne Legacies of Disease in Arabidopsis thaliana

Cited by 4 publications

References 17 publications

Coumarin biosynthesis genes are required after foliar pathogen infection for the creation of a microbial soil-borne legacy that primes plants for SA-dependent defenses

Coumarin biosynthesis genes are required after foliar pathogen infection for the creation of a microbial soil-borne legacy that primes plants for SA-dependent defenses

Plant microbiota dysbiosis and the Anna Karenina Principle

Congruent downy mildew-associated microbiomes reduce plant disease and function as transferable resistobiomes

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