Soil Bacteria as Potential Biological Control Agents of Fusarium Species Associated with Asparagus Decline Syndrome

Lastra, Eduardo de la; Camacho, M. E.; Capote, Nieves

doi:10.3390/app11188356

Cited by 10 publications

(6 citation statements)

References 42 publications

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“…It is also known that at least 4–5% genome of any strain of the B. subtilis is responsible for the production of antimicrobial compounds ( Stein, 2005 ). This species has also been identified as a good candidate for plant growth promotion and/or biocontrol of many plant diseases by different researchers ( Pandey, 2018a ; Caulier et al, 2019 ; De la Lastra et al, 2021 ). These organisms enhance plant growth and suppress plant diseases by different modes of action.…”

Section: Introductionmentioning

confidence: 99%

Macrolactin A mediated biocontrol of Fusarium oxysporum and Rhizoctonia solani infestation on Amaranthus hypochondriacus by Bacillus subtilis BS-58

Pandey

Prabha²,

Negi³

et al. 2023

Front. Microbiol.

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Plant diseases are one of the main hurdles for successful crop production and sustainable agriculture development world-wide. Though several chemical measures are available to manage crop diseases, many of them have serious side effects on humans, animals and the environment. Therefore, the use of such chemicals must be limited by using effective and eco-friendly alternatives. In view of the same, we found a Bacillus subtilis BS-58 as a good antagonist towards the two most devastating phytopathogens, i.e., Fusarium oxysporum and Rhizoctonia solani. Both the pathogens attack several agricultural crops (including amaranth) and induce a variety of infections in them. The findings of scanning electron microscopy (SEM) in this study suggested that B. subtilis BS-58 could inhibit the growth of both the pathogenic fungi by various means such as perforation, cell wall lysis, and cytoplasmic disintegration in the fungal hyphae. Thin-layer chromatography, LC–MS and FT-IR data revealed the antifungal metabolite to be macrolactin A with a molecular weight of 402 Da. Presence of the mln gene in the bacterial genome further endorsed that the antifungal metabolite produced by BS-58 was macrolactin A. Pot trial conducted in the present study showed that seed treatment by BS-58 effectively reduced seedling mortality (54.00 and 43.76%) in amaranth, when grown in pathogen infested soil (F. oxysporum and R. solani, respectively), when compared to their respective negative controls. Data also revealed that the disease suppression ability of BS-58 was almost equivalent to the recommended fungicide, carbendazim. SEM analysis of roots of the seedlings recovered from pathogenic attack substantiated the hyphal disintegration by BS-58 and prevention of amaranth crop. The findings of this study conclude that macrolactin A produced by B. subtilis BS-58 is responsible for the inhibition of both the phytopathogens and the suppression of the diseases caused by them. Being native and target specific, such strains under suitable conditions, may result in ample production of antibiotic and better suppression of the disease.

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Section: Introductionmentioning

confidence: 99%

Macrolactin A mediated biocontrol of Fusarium oxysporum and Rhizoctonia solani infestation on Amaranthus hypochondriacus by Bacillus subtilis BS-58

Pandey

Prabha²,

Negi³

et al. 2023

Front. Microbiol.

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Section: Methodsmentioning

confidence: 99%

“…These strains exhibited a proved antagonism against M. phaseolina 53,54 and were previously identified as Bacillus paralicheniformis Hvs2, Streptomyces fradiae Hvs6, Brevibacterium frigoritolerans Hvs8, Pseudomonas aeruginosa AC17, Bacillus velezensis ACH16, Bacillus velezensis FC37. 53,58 The second set of bacterial strains was isolated from healthy "disease escape" almond trees cv. Belona and Soleta grown in a commercial orchard with more than 90% of Botryosphaeria canker disease incidence (Alcalá del Río, Seville): Pantoea agglomerans Bel 1-1, Kocuria rhizophila Bel 1-3, Rhizobium soli Bel 1-4, Kocuria rhizophila Bel 1-5, Bacillus safensis subsp.…”

Section: Antagonistic Microorganismsmentioning

confidence: 99%

“…Rhizopheric bacterial strains from the species Bacillus velezensis, B. paralicheniformis, Brevibacterium tolerans, Streptomyces fradiae, and Pseudomonas aeruginosa, isolated from the rhizosphere of Arthrocaulon macrostachyum, Vaccinium corymbosum and Fragaria × ananassa plants, were previously identified. 53,58 Nine endophytic bacterial strains isolated from the subcortical tissue of "disease scape" almond trees were identified in this study by 16S rRNA sequencing, belonging to the species Bacillus mobilis, B. safensis subsp. safensis, Kocuria rhizophila, Neobacillus niacin, Novosphingobium barchaimii, Pantoea agglomerans, Pseudomanas ficuserectae and Rhizobium soli (Table 1).…”

Section: Bacterial Identificationmentioning

confidence: 99%

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Biocontrol of almond canker diseases caused by Botryosphaeriaceae fungi

Romero‐Cuadrado,

Picos,

Camacho

et al. 2023

Pest Management Science

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BACKGROUNDBotryosphaeria dieback is a canker disease caused by fungal species of the Botryosphaeriaceae family that threatens almond productivity. The most common control measure to prevent canker development is the application of fungicides which are being phased out by European Union regulations. In the present study, two sets of bacterial strains were evaluated for their antifungal activity against pathogenic Botryosphaeriaceae species through in vitro and in vivo antagonism assays.RESULTSThe rhizospheric bacteria Pseudomonas aeruginosa AC17 and Bacillus velezensis ACH16, as well as the endophytic bacteria Bacillus mobilis Sol 1–2, respectively inhibited 87, 95, and 63% of the mycelial growth of Neofusicoccum parvum, Botryosphaeria dothidea, Diplodia seriata, and Macrophomina phaseolina. Additionally, they significantly reduced the length of lesions caused by N. parvum and B. dothidea in artificially inoculated detached almond twigs. All these bacterial strains produce hydrolytic enzymes that are able to degrade the fungal cell wall. P. aeruginosa AC17 also produces toxic volatile compounds, such as hydrogen cyanide. This strain was the most effective in controlling Botryosphaeria dieback in planta under controlled conditions at a level similar to the biocontrol agent Trichoderma atroviride and standard chemical fungicide treatments.CONCLUSIONPseudomonas aeruginosa AC17 is the best candidate to be considered as a potential biocontrol agent against Botryosphaeriaceae fungi affecting almond. © 2023 Society of Chemical Industry.

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“…In addition, disease control by chemical pesticides is cost-effective for farmers in developing countries ( Wesseling et al, 1997 ; Bordoh et al, 2020 ). For these reasons, biological control using antagonistic microorganisms has emerged as environment-friendly alternative to control plant diseases ( Köhl et al, 2019 ; De la Lastra et al, 2021 ). Antagonistic microorganisms produce a variety of secondary metabolites to combat plant pathogens ( Hossain et al, 2015 ; Köhl et al, 2019 ; Cellini et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Bacillus tequilensis GYUN-300: An Antagonistic Bacterium Against Red Pepper Anthracnose Caused by Colletotrichum acutatum in Korea

et al. 2022

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Anthracnose is a fungal disease caused by Colletotrichum species and has detrimental effects on many crops, including red pepper. This study used Bacillus tequilensis GYUN-300 (GYUN-300), which exhibit antagonistic activity against the fungal pathogen, Colletotrichum acutatum. This pathogen causes anthracnose that manifests primarily as a fruit rot in red pepper. There have been little efforts to identify antagonistic bacteria from mushrooms; this strain of bacteria was identified as B. tequilensis using BIOLOG and 16S rDNA sequencing analysis. The genetic mechanism underpinning the biocontrol traits of GYUN-300 was characterized using the complete genome sequence of GYUN-300, which was closely compared to related strains. GYUN-300 inhibited mycelial growth and spore germination of C. acutatum under in vitro conditions. Important antagonistic traits, such as siderophore production, solubilization of insoluble phosphate, and production of lytic enzymes (cellulase, protease, and amylase), were observed in GYUN-300, These trains promoted growth in terms of seed germination and vigorous seedling growth compared to the non-treated control. When red pepper fruits were treated with GYUN-300, the preventive and curative effects were 66.6 and 38.3% effective, respectively, in wounded red pepper fruits; there was no difference between the preventive and curative effects in non-wounded red pepper fruits. Furthermore, GYUN-300 was resistant to several commercial fungicides, indicating that GYUN-300 bacterial cells may also be used synergistically with chemical fungicides to increase biocontrol efficiency. Based on in vitro results, GYUN-300 played a role to control anthracnose disease effectively in field conditions when compared to other treatments and non-treated controls. The results from this study provide a better understanding of the GYUN-300 strain as an effective biocontrol agent against red pepper anthracnose; this form of biocontrol provides an environment-friendly alternative to chemical fungicides.

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Soil Bacteria as Potential Biological Control Agents of Fusarium Species Associated with Asparagus Decline Syndrome

Cited by 10 publications

References 42 publications

Macrolactin A mediated biocontrol of Fusarium oxysporum and Rhizoctonia solani infestation on Amaranthus hypochondriacus by Bacillus subtilis BS-58

Macrolactin A mediated biocontrol of Fusarium oxysporum and Rhizoctonia solani infestation on Amaranthus hypochondriacus by Bacillus subtilis BS-58

Biocontrol of almond canker diseases caused by Botryosphaeriaceae fungi

Identification and Characterization of Bacillus tequilensis GYUN-300: An Antagonistic Bacterium Against Red Pepper Anthracnose Caused by Colletotrichum acutatum in Korea

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