Soil Bacteria and Fungi Respond on Different Spatial Scales to Invasion by the Legume Lespedeza cuneata

Yannarell, Anthony C.; Busby, Ryan R.; Denight, Michael L.; Gebhart, Dick L.; Taylor, Steven J.

doi:10.3389/fmicb.2011.00127

Cited by 39 publications

(40 citation statements)

References 68 publications

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“…First, OTU relative abundance within a sample was calculated by dividing the fluorescence intensity of an individual peak by the total intensity of the electrophoretic profile (sum of the intensity of all peaks). This step allows correcting for possible run to run variability in fluorescence signal that may arise during capillary electrophoresis (Yannarell et al, 2011). Data were then square-root transformed to minimize the influence of dominant OTUs on the sample ordination.…”

Section: Microbial Community Structurementioning

confidence: 99%

Combined effects of drought and the fungicide tebuconazole on aquatic leaf litter decomposition

et al. 2016

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Highlights• The combined effects of drought and the fungicide tebuconazole on aquatic leaf litter decomposition (LLD) were assessed.• TBZ applied alone had no significant effect on LLD.• Drought strongly impacted the microbial LLD process and led to cascading effects on Gammarus feeding.• Structural and functional effects increased when drought and TBZ were applied simultaneously. AbstractLoss of biodiversity and altered ecosystem functioning are driven by the cumulative effects of multiple natural and anthropogenic stressors affecting both quantity and quality of water resources. Here we performed a 40-day laboratory microcosm experiment to assess the individual and combined effects of drought and the model fungicide tebuconazole (TBZ) on leaf litter decomposition (LLD), a fundamental biogeochemical process in freshwater ecosystems. Starting out from a worst-case scenario perspective, leaf-associated microbial communities were exposed to severe drought conditions (four 5-day drought periods alternated with 4-day immersion periods) and/or a chronic exposure to TBZ (nominal concentration of 20 µg L−1). We assessed the direct effects of drought and fungicide on the structure (biomass, diversity) and activity (extracellular enzymatic potential) of fungal and bacterial assemblages colonizing leaves. We also investigated indirect effects on the feeding rates of the amphipod Gammarus fossarum on leaves previously exposed to drought and/or TBZ contamination. Results indicate a stronger effect of drought stress than fungicide contamination under the experimental conditions applied. Indeed, the drought stress strongly impacted microbial community structure and activities, inhibiting the LLD process and leading to cascading effects on macroinvertebrate feeding. However, despite the lack of significant effect of TBZ applied alone, the effects of drought on microbial functions (i.e., decrease in LLD and in enzymatic activities) and on Gammarus feeding rates were more pronounced when drought and TBZ stresses were applied together. In a perspective of ecological risk assessment and ecosystem management for sustainability, these findings stress the need for deeper insight into how multiple stressors can affect the functioning of aquatic ecosystems and associated services.

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Section: Microbial Community Structurementioning

confidence: 99%

Combined effects of drought and the fungicide tebuconazole on aquatic leaf litter decomposition

et al. 2016

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“…Greene var. fasciculata from previously described locations (Yannarell et al 2011) at Ft. Benning, Georgia and Ft. Leonard Wood, Missouri. Root nodules were cleaned with 0.02 % polysorbate 20, surface sterilized with 6 % sodium hypochlorite and then rinsed with autoclaved DI water three times (Bender et al 1989).…”

Section: Methodsmentioning

confidence: 99%

“…1). Furthermore, our previous work has shown that high densities of invasive L. cuneata are correlated with shifts in overall soil bacterial community composition in comparison to uninvaded sites (Yannarell et al 2011). Further research is necessary before it can be established that these belowground changes can reinforce L. cuneata invasion, but our current results indicate that changes to the composition of nitrogenfixing rhizobacteria in invaded habitats could lead to asymmetrical benefits for L. cuneata over its native congeners.…”

Section: Asymmetrical Benefit Of Microbial Mutualists To Native and Imentioning

confidence: 96%

“…rhizobacteria and mycorrhizal fungi (Reinhart and Callaway 2006)) might play an important role in invasion success (Klironomos 2002;Mitchell et al 2006;Reinhart and Callaway 2006;van der Putten et al 2007), few studies have reported how the interaction between L. cuneata and its associated microorganisms influences its invasion success. However, a recent study (Yannarell et al 2011) about L. cuneata suggests that soil bacteria communities in heavily invaded and uninvaded sites were significantly different, which raises the possibility that microorganisms associated with L. cuneata invasion may play a role in facilitating its invasion.…”

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confidence: 96%

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Invasive Lespedeza cuneata and native Lespedeza virginica experience asymmetrical benefits from rhizobial symbionts

Busby

Gebhart

et al. 2014

Plant Soil

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Background and Aims Lespedeza cuneata (Dum. Cours.) G. Don is an invasive legume that displaces populations of native N. American congeners. Our aims are to determine the growth benefits of different rhizobacterial strains for L. cuneata and native Lespedeza virginica (L.) Britton, and to determine if these strains influence competition between these plants. Methods Plants were grown under nitrogen-limiting conditions in sterilized soil in pairs consisting of two L. cuneata, two L. virginica, or one of each species, and then plants were inoculated with one of seven rhizobial isolates, or with a no-strain control. After 3 months, plants were harvested for determination of biomass and nodulation rate. Results Five of the assayed stains improved L. cuneata biomass over uninoculated controls, but none of the strains benefited L. virginica. L. cuneata plants had more biomass and root nodules when grown in competition with L. virginica than with a conspecific. Conclusions Asymmetrical benefits from these symbionts accrued to invasive L. cuneata but not to native L. virginica, and this may provide the invader with a growth advantage in the field. Changes in the availability of effective symbionts in the soils of invaded sites can shape performance of native and invasive plants.

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“…DGGE is one of the important approaches to study the shifts in soil microbial communities subject to various kinds of stresses. As such, this technique has been used in several studies carried out to assess the impact of invasive plant species on soil microbial community (e.g., Nie et al 2010;Yannarell et al 2011;Zhang et al 2010). The fundamental question asked in the present study was whether or not C. sumatrensis alters soil microbial communities in the urban ecosystems of the Kashmir Himalaya.…”

Section: Introductionmentioning

confidence: 99%

Invasion by Conyza sumatrensis alters soil microbial community structure in urban ecosystems

Rasool

Reshi

Khasa³

et al. 2016

Ecol Process

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Introduction: Whether invasive plants stimulate or inhibit the soil microbial diversity is still an open question. Despite large-scale invasion by Conyza sumatrensis (Retz.) E. Walker in the urban ecosystems of the Srinagar city of the Kashmir Himalayan region, limited information exists on its impact, particularly, on the belowground microbial diversity. The present study was thus conducted to compare the soil microbial (bacterial and ascomycetous fungal) diversity between the sites invaded by C. sumatrensis and un-invaded (control) sites. Methods: Soil metagenome was extracted from C. sumatrensis invaded and un-invaded plots at the three study sites. A total of six plots (5 × 5 m each in size), including three invaded by C. sumatrensis and three un-invaded plots were nested within each study site. DNA after amplification was subject to denaturing gradient gel electrophoresis (DGGE); the bands were extracted from the DGGE gel, re-amplified, and sequenced for identification of the species.

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Soil Bacteria and Fungi Respond on Different Spatial Scales to Invasion by the Legume Lespedeza cuneata

Cited by 39 publications

References 68 publications

Combined effects of drought and the fungicide tebuconazole on aquatic leaf litter decomposition

Combined effects of drought and the fungicide tebuconazole on aquatic leaf litter decomposition

Invasive Lespedeza cuneata and native Lespedeza virginica experience asymmetrical benefits from rhizobial symbionts

Invasion by Conyza sumatrensis alters soil microbial community structure in urban ecosystems

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