Soil applied Ca, Mg and B altered phyllosphere and rhizosphere bacterial microbiome and reduced Huanglongbing incidence in Gannan Navel Orange

Zhou, Yingjie; Tang, Yanni; Hu, Chengxiao; Zhan, Ting; Zhang, Simin; Cai, Miaomiao; Zhao, Xiaohu

doi:10.1016/j.scitotenv.2021.148046

Cited by 20 publications

(15 citation statements)

References 53 publications

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“…Recently, Zhou et al . ( 2021b , 2021 ) reported that the phyllosphere microbiome is involved in the transmission of antibiotic resistance genes in the urban green facade. Another report (Bárta et al ., 2021 ) suggests that the phyllosphere microbiome aids in the establishment of the invasive macrophyte Hydrilla verticillata L. under conditions of nitrogen scarcity.…”

Section: Ecological Functions Of the Phyllosphere Microbiomementioning

confidence: 99%

“…Furthermore, opening of leaf stomata and wounds provides a pathway for the transformation and migration between endophytes and epiphytes, and the opportunity for external microbes from aerosols and insects to colonize the plant, which also suggests that plants and the environment are interconnected (Mullens & Jamann, 2021 ; Xiang et al ., 2021 ). Nevertheless, a recent study analysed the sources of phyllosphere microbes through a customized microcosm that was able to control external microbes (Zhou et al ., 2021a , 2021 ), and showed that microbial sources from soil and air were limited. In another study, oak seeds were found to transmit a large part of microbes to roots and the phyllosphere, emphasizing that plant seeds are the reservoir of the plant microbiome (Abdelfattah et al ., 2021 ), particularly in the early stages of plant growth (Berg & Raaijmakers, 2018 ; X. Zhou et al ., 2020 ).…”

Section: Drivers and Sources Of The Phyllosphere Microbiomementioning

confidence: 99%

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Impacts of global change on the phyllosphere microbiome

et al. 2022

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Summary Plants form complex interaction networks with diverse microbiomes in the environment, and the intricate interplay between plants and their associated microbiomes can greatly influence ecosystem processes and functions. The phyllosphere, the aerial part of the plant, provides a unique habitat for diverse microbes, and in return the phyllosphere microbiome greatly affects plant performance. As an open system, the phyllosphere is subjected to environmental perturbations, including global change, which will impact the crosstalk between plants and their microbiomes. In this review, we aim to provide a synthesis of current knowledge of the complex interactions between plants and the phyllosphere microbiome under global changes and to identify future priority areas of research on this topic.

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Section: Ecological Functions Of the Phyllosphere Microbiomementioning

confidence: 99%

Section: Drivers and Sources Of The Phyllosphere Microbiomementioning

confidence: 99%

Impacts of global change on the phyllosphere microbiome

et al. 2022

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“…Macro-and micro-nutrients can further improve plant disease resistance against CLas pathogen indirectly through modification of microbial communities [40]. Plant microbiomes are the key agents involved in plant disease resistance [41].…”

Section: Discussionmentioning

confidence: 99%

Interactions between Indigenous Endophyte Bacillus subtilis L1-21 and Nutrients inside Citrus in Reducing Huanglongbing Pathogen Candidatus Liberibacter Asiaticus

Asad

et al. 2021

Pathogens

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Huanglongbing (HLB) pathogen Candidatus Liberibacter asiaticus (CLas) brings a great concern about the phloem nutrient transport in diseased plants. There is an urgent need to find the best management strategies to reduce the losses in the citrus industry worldwide. Endophytic bacteria are negatively affected by CLas pathogen, and these endophytes are associated with improved availability of nutrients and pathogen resistance. This study underpins the relationship between CLas pathogen, endophyte population and nutrients availability in citrus plants. The citrus plants were treated with Bacillus subtilis L1-21 and Hoagland solution to find out synergism efficacy to mitigate citrus HLB. We showed that citrus shoots in the presence of 50% Hoagland solution displayed maximum number of endophytes with 6.28 × 103 to 3.04 × 105 CFU/g. Among 50 candidate strains, B. subtilis L1-21 emerged as potential antagonist against surrogate strain Xanthomonas citri subsp. citri. The citrus half-leaf method identified that application of endophyte L1-21 with 50% Hoagland solution successfully reduces the CLas abundance. We point out that this combination results in a higher number of endophytes population with 2.52 × 104 to 9.11 × 106 CFU/g after 60 days, and reduces CLas pathogen abundance in asymptomatic HLB plants. In HLB symptomatic citrus plants, B. subtilis L1-21 potentially increases the endophyte population from 1.11 × 104 to 5.26 × 107 CFU/g in the presence of Hoagland solution, and pathogen abundance was reduced from 9.51 × 105 to 1.06 × 104 copies/g. Altogether, we suggested that the presence of endophyte L1-21 with Hoagland solution is more effective in HLB asymptomatic citrus plants, but a slight reduction of pathogen was observed in symptomatic plants. The findings revealed the role of indigenous citrus endophyte B. subtilis L1-21 along with other nutrients in the reduction of CLas pathogen abundance inside symptomatic and asymptomatic plants in citrus endophyte–nutrient–pathogen interplay.

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“…Actually, nutrients play a role in activating plant immunity system by altering the microbial community structure and the metabolism ( Sugimoto et al, 2010 ; Shi et al, 2012 ; Huber and Jones, 2013 ). A recent study has reported that application of calcium, magnesium, and boron to the soil can alter microbial structure and communities in phyllosphere and rhizosphere of HLB-affected citrus and promoted beneficial microorganism ( Burkholderiaceae , Xanthomonas , and Stenotrophomonas ) enrichment, which may have contributed to the reduced HLB incidence, and C Las titers ( Zhou et al, 2021 ).…”

Section: Role Of Citrus Microbiome In Combatting C ...mentioning

confidence: 99%

“…Currently, many strategies have been developed for HLB mitigation, including application of antimicrobials ( Zhang et al, 2011a , 2012 , 2014 , 2021 ; Hu and Wang, 2016 ; Hu et al, 2018 ; Yang et al, 2018 ), thermotherapy ( Hoffman et al, 2013 ; Fan et al, 2016 ; Yang et al, 2016b ; Doud et al, 2017 ; Ghatrehsamani et al, 2019 ; Vincent et al, 2019 ), macro-and micronutrients ( Spann and Schumann, 2009 ; Gottwald et al, 2012 ; Rouse et al, 2017 ; Mattos-Jr et al, 2020 ; Dong et al, 2021 ; Zhou et al, 2021 ), plant defense inducers ( Canales et al, 2016 ; Li et al, 2016 , 2019 , 2021a ; Hu et al, 2018 ; Wang, 2021 ), control of the insect vector ( Grafton-Cardwell et al, 2013 ; Boina and Bloomquist, 2015 ; Cocuzza et al, 2017 ; Pierre et al, 2021 ), biocontrol ( Trivedi et al, 2011 ; Hopkins and Wall, 2021 ; Nan et al, 2021 ; Poveda et al, 2021 ), and eradication of HLB symptomatic citrus trees ( Bassanezi et al, 2013 ; Yuan et al, 2020 ). However, these strategies have shown limited success in field applications and effective HLB management remains a challenge.…”

Section: Introductionmentioning

confidence: 99%

An Overview of the Mechanisms Against “Candidatus Liberibacter asiaticus”: Virulence Targets, Citrus Defenses, and Microbiome

Yang

Ancona

2022

Front. Microbiol.

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Citrus Huanglongbing (HLB) or citrus greening, is the most destructive disease for citrus worldwide. It is caused by the psyllid-transmitted, phloem-limited bacteria “Candidatus Liberibacter asiaticus” (CLas). To date, there are still no effective practical strategies for curing citrus HLB. Understanding the mechanisms against CLas can contribute to the development of effective approaches for combatting HLB. However, the unculturable nature of CLas has hindered elucidating mechanisms against CLas. In this review, we summarize the main aspects that contribute to the understanding about the mechanisms against CLas, including (1) CLas virulence targets, focusing on inhibition of virulence genes; (2) activation of citrus host defense genes and metabolites of HLB-tolerant citrus triggered by CLas, and by agents; and (3) we also review the role of citrus microbiome in combatting CLas. Finally, we discuss novel strategies to continue studying mechanisms against CLas and the relationship of above aspects.

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Soil applied Ca, Mg and B altered phyllosphere and rhizosphere bacterial microbiome and reduced Huanglongbing incidence in Gannan Navel Orange

Cited by 20 publications

References 53 publications

Impacts of global change on the phyllosphere microbiome

Impacts of global change on the phyllosphere microbiome

Interactions between Indigenous Endophyte Bacillus subtilis L1-21 and Nutrients inside Citrus in Reducing Huanglongbing Pathogen Candidatus Liberibacter Asiaticus

An Overview of the Mechanisms Against “Candidatus Liberibacter asiaticus”: Virulence Targets, Citrus Defenses, and Microbiome

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