Soil Application of a Formulated Biocontrol Rhizobacterium, Pseudomonas chlororaphis PCL1606, Induces Soil Suppressiveness by Impacting Specific Microbial Communities

Tienda, Sandra; Vida, Carmen; Lagendijk, Ellen; Weert, Sandra de; Linares, Irene; González-Fernández, Jorge; Guirado, Emilio; Vicente, Antonio de; Cazorla, Francisco M.

doi:10.3389/fmicb.2020.01874

Cited by 27 publications

(12 citation statements)

References 94 publications

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“…To unravel the behaviour of the Pc strains on the root scenario, the distribution pattern of fluorescent-tagged strains over the root surface viewed by confocal microscopy revealed a similar distribution pattern for the three strains when inoculated individually, colonizing the junctions of the epidemic cells of the roots, similar to that previously shown for the 06 strain ( Calderón et al., 2014 ). However, when competing with other strains, PCL1607 did not efficiently compete when PCL1601 was present, displaying a cell distribution in dispersed microcolonies along the root surface and alteration of its previously described individual colonization pattern which was stable over time, approaching levels of approximately 10 6 cfu/g root, which have been considered the carrying capacity for bacteria of the avocado root ( Tienda et al., 2020 ). These results revealed that, individually, these strains can easily adapt to the root environment without competition with another microorganism.…”

Section: Discussionmentioning

confidence: 94%

“…Routinely, the three wild-type Pseudomonas chlororaphis strains PCL1601, PCL1606 and PCL1607, and the derivative Pseudomonas spp. obtained were grown in lysogenic broth (LB) medium ( Bertani, 1951 ), tryptone peptone glycerol (TPG) medium ( Calderón et al., 2014 ), and M9 minimal medium supplemented with 10 mM succinate as a carbon source ( Miller, 1972 ; Tienda et al., 2020 ). Escherichia coli was grown on LB.…”

Section: Methodsmentioning

confidence: 99%

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Interplay between rhizospheric Pseudomonas chlororaphis strains lays the basis for beneficial bacterial consortia

Villar-Moreno

Tienda

Gutiérrez‐Barranquero

et al. 2022

Front. Plant Sci.

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Pseudomonas chlororaphis (Pc) representatives are found as part of the rhizosphere-associated microbiome, and different rhizospheric Pc strains frequently perform beneficial activities for the plant. In this study we described the interactions between the rhizospheric Pc strains PCL1601, PCL1606 and PCL1607 with a focus on their effects on root performance. Differences among the three rhizospheric Pc strains selected were first observed in phylogenetic studies and confirmed by genome analysis, which showed variation in the presence of genes related to antifungal compounds or siderophore production, among others. Observation of the interactions among these strains under lab conditions revealed that PCL1606 has a better adaptation to environments rich in nutrients, and forms biofilms. Interaction experiments on plant roots confirmed the role of the different phenotypes in their lifestyle. The PCL1606 strain was the best adapted to the habitat of avocado roots, and PCL1607 was the least, and disappeared from the plant root scenario after a few days of interaction. These results confirm that 2 out 3 rhizospheric Pc strains were fully compatible (PCL1601 and PCL1606), efficiently colonizing avocado roots and showing biocontrol activity against the fungal pathogen Rosellinia necatrix. The third strain (PCL1607) has colonizing abilities when it is alone on the root but displayed difficulties under the competition scenario, and did not cause deleterious effects on the other Pc competitors when they were present. These results suggest that strains PCL1601 and PCL1606 are very well adapted to the avocado root environment and could constitute a basis for constructing a more complex beneficial microbial synthetic community associated with avocado plant roots.

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Section: Discussionmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Interplay between rhizospheric Pseudomonas chlororaphis strains lays the basis for beneficial bacterial consortia

Villar-Moreno

Tienda

Gutiérrez‐Barranquero

et al. 2022

Front. Plant Sci.

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“…Kim et al (2019) demonstrated that Bacillus thuringiensis C25 exhibited strong antagonistic effects on mycelial growth by degrading the cell wall structures of R. necatrix mycelia. Recently, Tienda et al (2020) used a microbiome study to achieve an improved understanding of natural interactions between biocontrol agents and their impact on microbial communities. They observed that Pseudomonas chlororaphis (PCL1606) exhibited the highest biocontrol efficacy, and the biocontrol activity was directly correlated with the antifungal antibiotic 2-hexyl 5-propyl resorcinol, and the modified microbial communities.…”

Section: Antagonistic Bacteriamentioning

confidence: 99%

Current Status and Future Prospects of White Root Rot Management in Pear Orchards: A Review

Sawant¹,

Choi²,

Song

et al. 2021

Res. Plant Dis

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The current social demand for organic, sustainable, and eco-friendly approaches for farming, while ensuring the health and productivity of crops is increasing rapidly. Biocontrol agents are applied to crops to ensure biological control of plant pathogens. Research on the biological control of white root rot disease caused by a soil-borne pathogen, Rosellinia necatrix, is limited in pears compared to that in apple and avocado. This pathogenic fungus has an extensive host range, and symptoms of this disease include rotting of roots, yellowing and falling of leaves, wilting, and finally tree death. The severity of the disease caused by R. necatrix , makes it the most harmful fungal pathogen infecting the economical fruit tree species, such as pears, and is one of the main limiting factors in pear farming, with devastating effects on plant health and yield. In addition to agronomic and cultural practices, growers use chemical treatments to control the disease. However, rising public concern about environmental pollution and harmful effects of chemicals in humans and animals has facilitated the search for novel and environmentally friendly disease control methods. This review will briefly summarize the current status of biocontrol agents, ecofriendly methods, and possible approaches to control disease in pear orchards.

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“…or Bacillus spp. [ 23 , 24 , 25 ]; mycoviruses (Reovirus, Megabirnavirus and Hypovirus) [ 26 , 27 , 28 ]; and integrated management with the use of antagonistic fungi plus antagonistic bacteria [ 29 ], antagonistic rhizobacteria, Trichoderma spp. or non-pathogenic R. necatrix plus fungicide [ 30 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Novel Promising Citrus Rootstocks to White Root Rot

Arjona-López

Gmitter

Romero-Rodríguez

et al. 2022

Plants

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Citrus is one of the most important fruit crops in Mediterranean countries such as Spain, which is one of the main citrus-producing countries worldwide. Soil-borne pathogens, such as Rosellinia necatrix, are relevant limiting biotic factors in fruit trees, due to their tricky management. This fungus is a polyphagous plant pathogen with worldwide distribution, causing white root rot in woody crops, including citrus trees in Spain. The objective of this study was to evaluate the tolerance of new plant material against R. necatrix infection. Therefore, plants of 12 different citrus rootstocks were inoculated with one R. necatrix isolate. During the assay, and periodically, above-ground symptoms and chlorophyll content were evaluated. At the end of the experiment, leaf area and plant biomass measures were obtained. Rootstocks B11R5T64 and B11R5T60 achieved the lowest disease incidence of symptoms and reduction of biomass, and were similar to their respective controls in chlorophyll content and leaf area. Carrizo citrange, CL-5146 and UFR-5 were the most affected rootstocks in symptoms and biomass reduction. This work provides information about R. necatrix-tolerant citrus rootstocks, which can constitute a new integrated, sustainable and effective long-term strategy to avoid white root rot.

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Soil Application of a Formulated Biocontrol Rhizobacterium, Pseudomonas chlororaphis PCL1606, Induces Soil Suppressiveness by Impacting Specific Microbial Communities

Cited by 27 publications

References 94 publications

Interplay between rhizospheric Pseudomonas chlororaphis strains lays the basis for beneficial bacterial consortia

Interplay between rhizospheric Pseudomonas chlororaphis strains lays the basis for beneficial bacterial consortia

Current Status and Future Prospects of White Root Rot Management in Pear Orchards: A Review

Susceptibility of Novel Promising Citrus Rootstocks to White Root Rot

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