SOI-nanowire biosensor for the detection of D-NFAT 1 protein

Malsagova, Kristina A.; Ivanov, Yu. D.; Pleshakova, Tatyana O.; Kozlov, Andrey F.; Krohin, N. V.; Kaysheva, Anna L.; Shumov, Ivan D.; Popov, V.; Naumova, O. V.; Fomin, B. I.; Nasimov, D. A.

doi:10.18097/pbmc20156104462

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…It is used to select the initial current in the source-drain circuit [15]. The source of the input signal is various ions, molecules, proteins, DNA, etc., which are present in the analyzed solution and partially adsorbed on the surface of the first gate-NW [11][12][13][14]. Adsorption of biological molecules on the NW surface leads to a change in the concentration of charge carried in it and, accordingly, modulation of the current, i.e., biosensor response.…”

Section: Methodsmentioning

confidence: 99%

“…The fundamental principles of these devices are the laws of physics and chemistry, using the understanding of the transfer of the analyte as a result of its diffusion and convection and controlling the kinetics of binding and chemical reactions in the sample under study. Various biosensors, along with their characteristics, sensitivity, and design elements, are presented in the literature [11][12][13][14][15][16][17][18][19][20][21][22][23]. However, the problems of particle detection probability using sensors have received little attention in the literature.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor

Generalov,

Cheremiskina,

Glukhov

et al. 2023

Sensors

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The SOI-FET biosensor (silicon-on-insulator field-effect transistor) for virus detection is a promising device in the fields of medicine, virology, biotechnology, and the environment. However, the applications of modern biosensors face numerous problems and require improvement. Some of these problems can be attributed to sensor design, while others can be attributed to technological limitations. The aim of this work is to conduct a theoretical investigation of the “antibody + antigen” complex (AB + AG) detection processes of a SOI-FET biosensor, which may also solve some of the aforementioned problems. Our investigation concentrates on the analysis of the probability of AB + AG complex detection and evaluation. Poisson probability density distribution was used to estimate the probability of the adsorption of the target molecules on the biosensor’s surface and, consequently, to obtain correct detection results. Many implicit and unexpected causes of error detection have been identified for AB + AG complexes using SOI-FET biosensors. We showed that accuracy and time of detection depend on the number of SOI-FET biosensors on a crystal.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor

Generalov,

Cheremiskina,

Glukhov

et al. 2023

Sensors

View full text Add to dashboard Cite

show abstract

Investigation Detection of Antibody+Antigen Complexes by SOI-FET Biosensor

Генералов¹,

Cheremiskina²,

Glukhov³

et al. 2023

Preprint

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Detection of virus in sample is an actual problem in biotechnology, virology, medicine using biosensor. History of problem dates back to middle of last century and is associated with development of an ion-sensitive solid-state device for neurophysiological measurements. Aim of work is theoretical investigation process of detection antibody+antigen (AB+AG) complexes using SOI-FET biosensor. On example of crystal with ten biosensors, estimates of probability detection AB+AG complexes influenza virus are made. In detection process, following four results are possible: – target complex is present in test sample, and it was correctly detected; – target complex is present in sample, but it was not detected; – target complex is absent in sample, but background particles are mistakenly detected as target complex; – target complex is absent in sample, background particles present in sample and are correctly detected as noise.

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Nanowire biosensor p-type with immobilized antibodies for highly sensitive registration of the molecules of HCVcoreAg, a protein marker of viral hepatitis С

Malsagova¹,

Pleshakova²,

Galiullin³

et al. 2017

Nauchno-Prakticheskii Zhurnal «Patogenez»

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Цель исследования: обнаружение маркера гепатита С - ядерного белка HCVcoreAg с помощью нанопроводного детектора на основе структур «кремний-на-изоляторе» (КНИ-НП) с р-типом проводимости, модифицированной антителами. Методика: были использованы КНИ-структуры с p-типом проводимости. Толщина отсеченного слоя кремния составляла 32 нм, скрытого окисла (buried oxide, BOX) - 300 нм. В экспериментах ширина сенсоров составляла w = 3 мкм, толщина t = 32 нм, длина l = 10 мкм, число нанопроводов на кристалле 12. Поверхность нанопроводов (НП) модифицировалась в парах аминопропилтриэтоксисилана (APTES). Антитела против HCVcoreAg были ковалентно иммобилизованы на модифицированную НП-поверхность с использованием кросс-линкера дитиобис (сульфосукцинимидил пропионата) (DTSSP). В измерениях была использована жидкостная кювета объемом 500 мкл, дном которой являлся кристалл с НП-структурами. Диаметр чувствительной зоны составлял ~2 мм. Перемешивание раствора в кювете осуществлялось с помощью мешалки при скорости 3000 об./мин. Электрические измерения проводились с помощью пикоамперметра фирмы Keithley (model 6487, Keithley, http://www.keithley.com). Результаты: для НП-биосенсора с КНИ-НП p-типа с иммобилизованными антителами показана возможность регистрации HCVcoreAg в нейтральном и кислом буферных растворах. Минимальная концентрация HCVcoreAg, при которой был обнаружен белок, составила 10М. Заключение: Показано, что с помощью биосенсора на базе нанопроводов p-типа с иммобилизованными антителами может быть обнаружен в растворе биомаркер вирусного гепатита С без использования меток, в режиме реального времени. Концентрационная чувствительность анализа составила порядка 10 М. Aim. To detect HCVcoreAg using a nanowire detector based on silicon-on-insulator structures (SOI-NW) with p-type conductivity with immobilized antibodies. Methods. Silicon-on-insulator (SOI) structures with p-type conductivity were used. The cut-off layer thickness was 32 nm; the buried oxide (BOX) layer thickness was 300 nm. In the experiments, the sensor width was w = 3 mm, the thickness was t = 32 nm, the length was l = 10 mm, and the number of nanowires (NWs) on the crystal was 12. The surface of NWs was modified in aminopropyltriethoxysilane (APTES) vapor. Antibodies against HCVcoreAg were covalently immobilized onto the modified NW surface using a dithiobis (sulfosuccinimidyl propionate) (DTSSP) crosslinker. Throughout the measurements, a measuring cell (500 mL volume), whose bottom was a crystal with NW structures, was used. The diameter of sensor area was ~2 mm. The solution in the cell was stirred at 3000 rpm. Electrical measurements were conducted using a Keithley picoampermeter. Results. The study demonstrated that the NW biosensor with p-type SOI-NWs with immobilized antibodies was capable for detecting HCVcoreAg in buffer solutions with neutral and acidic pH. The lowest HCVcoreAg concentration, at which the protein was detectable, was 10 М. Conclusion. The viral hepatitis C biomarker can be detected in solutions in real time using a biosensor based on p-type NWs with immobilized antibodies, without using labels. The concentration sensitivity of the analysis was of the order of 10 M.

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SOI-nanowire biosensor for the detection of D-NFAT 1 protein

Cited by 4 publications

References 29 publications

Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor

Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor

Investigation Detection of Antibody+Antigen Complexes by SOI-FET Biosensor

Nanowire biosensor p-type with immobilized antibodies for highly sensitive registration of the molecules of HCVcoreAg, a protein marker of viral hepatitis С

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