Soft X-ray spectromicroscopy beamline at the CLS: Commissioning results

Kaznatcheev, Konstantine; Karunakaran, C.; Lanke, U. D.; Urquhart, Stephen G.; Obst, Martin; Hitchcock, Adam P.

doi:10.1016/j.nima.2007.08.083

Cited by 235 publications

(192 citation statements)

References 1 publication

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“…STXM and NEXAFS spectroscopy measurements were performed at the spectromicroscopy beamline 10ID1 of the Canadian Light Source (CLS, Saskatoon, SK) 63 . The STXM instrument uses a Fresnel zone plate to focus the X-ray to a spot size of 30 nm.…”

Section: Methodsmentioning

confidence: 99%

Experimental diagenesis of organo-mineral structures formed by microaerophilic Fe(II)-oxidizing bacteria

et al. 2015

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Twisted stalks are organo-mineral structures produced by some microaerophilic Fe(II)-oxidizing bacteria at O 2 concentrations as low as 3 mM. The presence of these structures in rocks having experienced a diagenetic history could indicate microbial Fe(II)-oxidizing activity as well as localized abundance of oxygen at the time of sediment deposition. Here we use spectroscopy and analytical microscopy to evaluate if-and what kind of-transformations occur in twisted stalks through experimental diagenesis. Unique mineral textures appear on stalks as temperature and pressure conditions increase. Haematite and magnetite form from ferrihydrite at 170°C-120 MPa. Yet the twisted morphology of the stalks, and the organic matrix, mainly composed of long-chain saturated aliphatic compounds, are preserved at 250°C-140 MPa. Our results suggest that iron minerals might play a role in maintaining the structural and chemical integrity of stalks under diagenetic conditions and provide spectroscopic signatures for the search of ancient life in the rock record.

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Section: Methodsmentioning

confidence: 99%

Experimental diagenesis of organo-mineral structures formed by microaerophilic Fe(II)-oxidizing bacteria

et al. 2015

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“…A plane grating solution is used for the x-ray monochromatization, which maintains the polarization state over the whole energy range covered by the monochromator. The possibility of selecting the so-called c ffparameter allows balancing between energy resolution, photon flux or higher order suppression [2,3,4]. The energy resolution was characterized using a gas cell, by measuring the photo ion yield.…”

Section: The Beamline: a Cpgm For Optimal Photon Flux And Energy Resomentioning

confidence: 99%

Description of the new I1011 beamline for magnetic measurements using synchrotron radiation at MAX-lab

Kowalik

Öhrwall

Jensen

et al. 2010

J. Phys.: Conf. Ser.

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“…X-ray imaging and spectromicroscopy of the biofilms prepared as described above were carried out using STXM microscopes on beamlines 5.3.2.2 at the Advanced Light Source (ALS) (Berkeley, CA) (39) and the SM beamline 10ID-1 at the Canadian Light Source (CLS) (Saskatoon, Saskatchewan, Canada) (40). Near-edge X-ray fine structure absorption spectra (NEXAFS) were collected at the C 1s edge at a single energy or as a sequence of energies (i.e., an 80-to 100-image stack) from 280 to 320 eV.…”

Section: Methodsmentioning

confidence: 99%

Microscopic and Spectroscopic Analyses of Chlorhexidine Tolerance in Delftia acidovorans Biofilms

Rema

Lawrence

Dynes

et al. 2014

Antimicrob Agents Chemother

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The physicochemical responses of Delftia acidovorans biofilms exposed to the commonly used antimicrobial chlorhexidine (CHX) were examined in this study. A CHX-sensitive mutant (MIC, 1.0 g ml ؊1 ) was derived from a CHX-tolerant (MIC, 15.0 g ml ؊1 ) D. acidovorans parent strain using transposon mutagenesis. D. acidovorans mutant (MT51) and wild-type (WT15) strain biofilms were cultivated in flow cells and then treated with CHX at sub-MIC and inhibitory concentrations and examined by confocal laser scanning microscopy (CLSM), scanning transmission X-ray microscopy (STXM), and infrared (IR) spectroscopy. Specific morphological, structural, and chemical compositional differences between the CHX-treated and -untreated biofilms of both strains were observed. Apart from architectural differences, CLSM revealed a negative effect of CHX on biofilm thickness in the CHX-sensitive MT51 biofilms relative to those of the WT15 strain. STXM analyses showed that the WT15 biofilms contained two morphochemical cell variants, whereas only one type was detected in the MT51 biofilms. The cells in the MT51 biofilms bioaccumulated CHX to a similar extent as one of the cell types found in the WT15 biofilms, whereas the other cell type in the WT15 biofilms did not bioaccumulate CHX. STXM and IR spectral analyses revealed that CHX-sensitive MT51 cells accumulated the highest levels of CHX. Pretreating biofilms with EDTA promoted the accumulation of CHX in all cells. Thus, it is suggested that a subpopulation of cells that do not accumulate CHX appear to be responsible for greater CHX resistance in D. acidovorans WT15 biofilm in conjunction with the possible involvement of bacterial membrane stability.

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Soft X-ray spectromicroscopy beamline at the CLS: Commissioning results

Cited by 235 publications

References 1 publication

Experimental diagenesis of organo-mineral structures formed by microaerophilic Fe(II)-oxidizing bacteria

Experimental diagenesis of organo-mineral structures formed by microaerophilic Fe(II)-oxidizing bacteria

Description of the new I1011 beamline for magnetic measurements using synchrotron radiation at MAX-lab

Microscopic and Spectroscopic Analyses of Chlorhexidine Tolerance in Delftia acidovorans Biofilms

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