Soft X-ray contact microscopy of nematode Caenorhabditis elegans

Poletti, G.; Orsini, F.; Batani, D.; Bernardinello, A.; Desai, T.; Ullschmied, J.; Skála, J.; Králíková, B.; Krouský, E.; Juha, L.; Pfeifer, M.; Kadlec, C.; Mocek, Tomáš; Präg, A. R.; Renner, O.; Cotelli, Franco; Lamia, C. Lora; Zullini, A.

doi:10.1140/epjd/e2004-00084-4

Cited by 10 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sample was a Caenorhabditis elegans (Maupas 1899) microradiograph from the experiment performed by Poletti et al (2004). The sample was prepared by placing a water droplet containing several individuals of C. elegans on the sample holder (a silicon dice with a silicon nitride window), which was then exposed to a subnanosecond flash of soft x-rays generated by the iodine laser source PALS (Prague Asterix Laser System, Prague, Czechoslovakia).…”

Section: Methodsmentioning

confidence: 99%

“…There are reports on the application of SXCM-AFM of cells (yeast cells, unicellular algae, and human red blood cells) (Batani et al 2000, Larabell and Le Gros 2004, Masini et al 1997, Milani et al 2004a, Stead et al 1995. Poletti et al (2004) used SXCM-AFM for imaging the nematode Caenorhabditis elegans as a model for imaging of a complex, multicellular organisms. They obtained the SXCM radiograph of a whole C. elegans and then imaged this shadowgram by combining several tens of AFM images; they were able to identify a variety of morphologic features.…”

Section: Introductionmentioning

confidence: 99%

“…The FIB was used in microscopy mode for imaging the microradiograph at different magnifications. The method was applied on C. elegans and the results compared with those obtained by Poletti et al (2004).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Read-out of soft x-ray contact microscopy microradiographs by focused ion beam/scanning electron microscope

Milani

Drobne

Tatti³

et al. 2005

Scanning

View full text Add to dashboard Cite

Summary:A novel focused ion beam-based technique is presented for the read-out of microradiographs of Caenorhabditis elegans nematodes generated by soft x-ray contact microscopy (SXCM). In previous studies, the read-out was performed by atomic force microscopy (AFM), but in our work SXCM microradiographs were imaged by scanning ion microscopy (SIM) in a focused ion beam/scanning electron microscope (FIB/SEM). It allows an ad libitum selection of a sample region for gross morphologic to nanometric investigations, with a sequence of imaging and cutting. The FIB/SEM is less sensitive to height variation of the relief, and sectioning makes it possible to analyse the sample further. The SXCM can be coupled to SIM in a more efficient and faster way than to AFM. Scanning ion microscopy is the method of choice for the read-out of microradiographs of small multicellular organisms.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Read-out of soft x-ray contact microscopy microradiographs by focused ion beam/scanning electron microscope

Milani

Drobne

Tatti³

et al. 2005

Scanning

View full text Add to dashboard Cite

show abstract

“…Water window soft X-ray contact microscopy (WW SXCM), with a photon energy in the range of 280-530 eV, has been a widely used technique during the last 10 years (Albertano et al, 1997;Batani et al, 2002;Bollanti et al, 1998;Ford et al, 1991;Forster et al, 1992;Panessa et al, 1981;Poletti et al, 2004;Stead et al, 1988) for imaging of biological living samples with a resolution higher than that of optical microscopy. In comparison with other powerful imaging techniques, like transmission electron microscopy (TEM), it reduces the influence of several drawbacks.…”

Section: Introductionmentioning

confidence: 99%

High‐resolution water window X‐ray imaging of in vivo cells and their products using LiF crystal detectors

Bonfigli

Faenov

Flora

et al. 2007

Microscopy Res & Technique

View full text Add to dashboard Cite

High contrast imaging of in vivo Chlorella sorokiniana cells with submicron spatial resolution was obtained with a contact water window X-ray microscopy technique using a point-like, laser-plasma produced, water-window X-ray radiation source, and LiF crystals as detectors. This novel type of X-ray imaging detectors is based on photoluminescence of stable electronic point defects, characterized by high intrinsic resolution. The fluorescence images obtained on LiF crystals exposed in single-shot experiments demonstrate the high sensitivity and dynamic range of this new detector. The powerful performances of LiF crystals allowed us to detect the exudates of Chlorella cells in their living medium and their spatial distribution in situ, without any special sample preparation.

show abstract

“…In recent years, X-rays generated from high-power laserproduced plasmas have gained much importance, and have been applied in several fields including material dynamics (Rousse et al, 2001;Boschetto et al, 2006;Isakov et al, 2005;Schollmeier et al, 2006), radiobiology (Turcu et al, 1994a;Bortolotto et al, 2000), the study of fundamental interaction of intense X-rays with matter (Batani et al, 2002), micro-lithography (Turcu et al, 1994b), studies of threshold of DNA damage (Turcu et al, 1994a), of radiation induced damage to cell metabolism (Masini et al, 1999;Milani et al, 1999), water-window X-ray microscopy Desai et al, 2003Desai et al, , 2004Poletti et al, 2004), diagnostics of laser produced plasmas i.e., determination of plasma density and temperature (Koenig et al, 1997;Magunov et al, 1998;Batani et al, 1999;Pisani et al, 1999;Adamek et al, 2006;Korobkin et al, 2005;Kawamura et al, 2006), identification of atomic processes (Stepanov et al, 1997;Rosmej et al, 1997;Biemont et al, 2000), etc.…”

Section: Introductionmentioning

confidence: 99%

High resolution X-ray emission spectra from picosecond laser irradiated Ge targets

et al. 2007

Self Cite

View full text Add to dashboard Cite

Investigations of a high resolution X-ray emission spectrum in the range 0.66-0.75 nm obtained by irradiating a Germanium target with high-power p-polarized, 40 picosecond laser radiation at 532 nm wavelength was done. Spectra in the wavelength region of 2l-4l 0 and 2l-5l 0 L-shell transitions in F-like, Ne-like and Na-like germanium ions were recorded using the FSSR-2D spectrometer equipped with a spherically bent quartz crystal with a spectral resolution l/Dl better than 5000. Spectral lines were compared with theoretical values obtained using the LANL plasma kinetic code ATOMIC. Fair agreement between experimental and theoretical spectral lines has been observed, which allowed to measure enough high bulk electron temperature values of 560 eV and electron density of $10 21 cm 23 in Ge plasma irradiated by rather small commercial high repetition rate Nd:YAG laser system.

show abstract

Soft X-ray contact microscopy of nematode Caenorhabditis elegans

Cited by 10 publications

References 15 publications

Read-out of soft x-ray contact microscopy microradiographs by focused ion beam/scanning electron microscope

Read-out of soft x-ray contact microscopy microradiographs by focused ion beam/scanning electron microscope

High‐resolution water window X‐ray imaging of in vivo cells and their products using LiF crystal detectors

High resolution X-ray emission spectra from picosecond laser irradiated Ge targets

Contact Info

Product

Resources

About