Soft Tissue Principles to Minimize Scarring

Welshhans, Jamie L.; Hom, David B.

doi:10.1016/j.fsc.2016.08.002

Cited by 11 publications

(5 citation statements)

References 10 publications

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“…Scarring is often problematic for patients, especially on exposed parts of the body, such as the craniofacial region or extremities. Surgical approximation of open wound tissues has been traditionally performed to minimize scarring ( Welshhans and Hom, 2017 ). Currently, surgical skin incisions are routinely closed by suturing; however, the surgical closure of a dermal wound does not guarantee adequate approximation of the entire length of the wound.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing

Shibuya

Okawa

Kondo

et al. 2022

eLife

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Attempts to minimize scarring remain among the most difficult challenges facing surgeons, despite the use of optimal wound closure techniques. Previously, we reported improved healing of dermal excisional wounds in circadian clock neuronal PAS domain 2 (Npas2)-null mice. In this study, we performed high-throughput drug screening to identify a compound that downregulates Npas2 activity. The hit compound (Dwn1) suppressed circadian Npas2 expression, increased murine dermal fibroblast cell migration, and decreased collagen synthesis in vitro. Based on the in vitro results, Dwn1 was topically applied to iatrogenic full-thickness dorsal cutaneous wounds in a murine model. The Dwn1-treated dermal wounds healed faster with favorable mechanical strength and developed less granulation tissue than the controls. The expression of type I collagen, Tgfb1, and a-smooth muscle actin was significantly decreased in Dwn1-treated wounds, suggesting that hypertrophic scarring and myofibroblast differentiation are attenuated by Dwn1 treatment. NPAS2 may represent an important target for therapeutic approaches to optimal surgical wound management.

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Section: Discussionmentioning

confidence: 99%

Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing

Shibuya

Okawa

Kondo

et al. 2022

eLife

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“…It is speculated that the development of benign airway stenosis is related to the activation of neutrophils. It has been found that neutrophils can gather to the wound site within 24 h after injury ( Welshhans and Hom, 2017 ), release inflammatory mediators and cytokines such as TNF- α, IL-1 and IL-6, enhance inflammatory response, and stimulate vascular endothelial growth factor and IL-8 production, which all serve to facilitate injury repair ( Reinke and Sorg, 2012 ). However, neutrophils also produce a large amount of oxygen free radicals, which can cause oxidative stress in wound tissue, delay the repair process and change the healing result ( Koh and DiPietro, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Construction and Comprehensive Analysis of the ceRNA Network to Reveal Key Genes for Benign Tracheal Stenosis

Zou

Cai

2022

Front. Genet.

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Objective: To explore the possible biological functions of the differentially expressed genes in patients with benign tracheal stenosis, and to provide a valuable molecular basis for investigating the pathogenesis of benign tracheal stenosis.Method: Whole transcriptome sequencing was performed on blood samples collected from patients with benign tracheal stenosis and normal controls. Differentially expressed mRNA, lncRNA, and circRNA were analyzed using the DESeq2 package. The protein interaction networks for differentially expressed mRNAs were constructed by STRING. The results of gene co-expression network analysis, Starbase database prediction, and differential gene expression were combined to construct a competing endogenous RNA network. The transcription factors of key genes were predicted using the Network Analyst database and a transcription factor-mRNA regulatory network was constructed. The classical pathways, intermolecular interaction networks, and upstream regulatory components of key genes were analyzed using Ingenuity Pathway Analysis (IPA). Finally, the DGIDB database was used to predict the potential therapeutic drugs to target the identified key genes.Result: Based on mRNA, lncRNA and circRNA expression data, we found that differentially expressed mRNAs were enriched in oxygen transport, neutrophil activation, immune response, and oxygen binding. Then the pearson correlation between mRNAs of 46 key genes and lncRNAs and cricRNAs were calculated, and the correlation greater than 0.9 were selected to construct the co-expression network of “mRNA-lncRA” and “mRNA-cricRNA.” Moreover, a “lncRNA-miRNA-mRNA” network and a “circRNA-miRNA-mRNA” network were constructed. IPA analysis showed that the 46 key genes were significantly associated with inflammatory activation and acute respiratory distress syndrome. The constructed TF-mRNA regulatory network was composed of 274 nodes and 573 interacting pairs. 251 potential therapeutic drugs were identified from the DGIDB database.Conclusion: This study analyzed the differential genes associated with benign tracheal stenosis and explored the potential regulatory mechanisms, providing a scientific reference for further studies on the pathogenesis of benign tracheal stenosis.

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“…Scarring is often problematic for patients, especially on exposed parts of the body, such as the craniofacial region or extremities. Surgical approximation of open wound tissues has been traditionally performed to minimize scarring (45). Currently, surgical skin incisions are routinely closed by suturing; however, the surgical closure of a dermal wound does not guarantee adequate approximation of the entire length of the wound.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing

Shibuya¹,

Okawa²,

Kondo³

et al. 2021

Preprint

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Attempts to minimize scarring remain among the most difficult challenges facing surgeons, despite the use of optimal wound closure techniques. Previously, we reported improved healing of dermal excisional wounds in circadian clock neuronal PAS domain 2 (Npas2)-null mice. In this study, we performed high-throughput drug screening to identify a compound that downregulates Npas2 activity. The hit compound (Dwn1) suppressed circadian Npas2 expression, increased murine dermal fibroblast cell migration, and decreased collagen synthesis in vitro. Based on the in vitro results, Dwn1 was topically applied to iatrogenic full-thickness dorsal cutaneous wounds in a murine model. The Dwn1-treated dermal wounds healed faster with favorable mechanical strength and developed less granulation tissue than the controls. The expression of type I collagen, Tgfβ1, and α-smooth muscle actin was significantly decreased in Dwn1-treated wounds, suggesting that hypertrophic scarring and myofibroblast differentiation are attenuated by Dwn1 treatment. NPAS2 may represent an important target for therapeutic approaches to optimal surgical wound management.

show abstract

Soft Tissue Principles to Minimize Scarring

Cited by 11 publications

References 10 publications

Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing

Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing

Construction and Comprehensive Analysis of the ceRNA Network to Reveal Key Genes for Benign Tracheal Stenosis

Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing

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