Soft tissue adhesive composed of modified gelatin and polysaccharides

Mo, Xiumei; Iwata, Hiroo; Matsuda, Shunji; Ikada, Yoshito

doi:10.1163/156856200743742

Cited by 98 publications

(57 citation statements)

References 16 publications

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“…2a). The terminal aldehyde group in DXTA reacts with resident amine groups in proteins forming an imine (15). Furthermore, the aldehyde groups of DXTA can form a hemiacetal with free hydroxyl groups from the glycerol subunit of our PGSA polymer surface.…”

Section: Resultsmentioning

confidence: 99%

A biodegradable and biocompatible gecko-inspired tissue adhesive

Mahdavi

Ferreira

Sundback

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

502

359

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There is a significant medical need for tough biodegradable polymer adhesives that can adapt to or recover from various mechanical deformations while remaining strongly attached to the underlying tissue. We approached this problem by using a polymer poly(glycerol-co-sebacate acrylate) and modifying the surface to mimic the nanotopography of gecko feet, which allows attachment to vertical surfaces. Translation of existing gecko-inspired adhesives for medical applications is complex, as multiple parameters must be optimized, including: biocompatibility, biodegradation, strong adhesive tissue bonding, as well as compliance and conformability to tissue surfaces. Ideally these adhesives would also have the ability to deliver drugs or growth factors to promote healing. As a first demonstration, we have created a gecko-inspired tissue adhesive from a biocompatible and biodegradable elastomer combined with a thin tissue-reactive biocompatible surface coating. Tissue adhesion was optimized by varying dimensions of the nanoscale pillars, including the ratio of tip diameter to pitch and the ratio of tip diameter to base diameter. Coating these nanomolded pillars of biodegradable elastomers with a thin layer of oxidized dextran significantly increased the interfacial adhesion strength on porcine intestine tissue in vitro and in the rat abdominal subfascial in vivo environment. This gecko-inspired medical adhesive may have potential applications for sealing wounds and for replacement or augmentation of sutures or staples.chemical cross-link ͉ medical adhesive ͉ nanotopography ͉ surgical suture

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Section: Resultsmentioning

confidence: 99%

A biodegradable and biocompatible gecko-inspired tissue adhesive

Mahdavi

Ferreira

Sundback

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

502

359

View full text Add to dashboard Cite

show abstract

“…(Belowich and Stoddart, 2012). They have already been used in formalin cross-linked synthetic medical adhesives (Paez and Jorge-Herrero, 2006), and other workers have created useful sealants, adhesives and gels from oxidized polymers that then form Schiff bases with amines on other polymers (Mo et al, 2000;Dawlee et al, 2005;Artzi et al, 2009).…”

Section: Cross-links Resulting From Protein Oxidationmentioning

confidence: 99%

The relative contribution of calcium, zinc and oxidation-based cross-links to the stiffness ofArion subfuscusglue

Braun

Menges

Opoku

et al. 2012

Journal of Experimental Biology

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SUMMARY. Thus these ions probably play a primary role in bringing polymers together directly. Imine bonds formed as a result of protein oxidation also contribute substantially to the stiffness of the glue. Disrupting these bonds with hydroxylamine caused a 33% decrease in storage modulus of the glue, while stabilizing them by reduction with sodium borohydride increased the storage modulus by 40%. Thus a combination of metal-based bonds operates in this glue. Most likely, cross-links directly involving calcium play a primary role in bringing together and stabilizing the polymer network, followed by imine bond formation and possible iron coordination.

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“…The scheme for hydrogel preparation was presented in Figure 1A. The gel time, which was defined as the time taken for the formed gel to stop the stir bar according to Balakrishnan and Jayakrishnan 23 and Mo et al, 24 was recorded using a stopwatch. The gel properties were adjusted by the weight content of nEOAs in eGels.…”

Section: Preparation Of Electroactive Neoasmentioning

confidence: 99%

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Wang¹,

Wang²,

Teng³

2016

IJN

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Injectable electroactive hydrogels (eGels) are promising in regenerative medicine and drug delivery, however, it is still a challenge to obtain such hydrogels simultaneously possessing other properties including uniform structure, degradability, robustness, and biocompatibility. An emerging strategy to endow hydrogels with desirable properties is to incorporate functional nanoparticles in their network. Herein, we report the synthesis and characterization of an injectable hydrogel based on oxidized alginate (OA) crosslinking gelatin reinforced by electroactive tetraaniline-graft-OA nanoparticles (nEOAs), where nEOAs are expected to impart electroactivity besides reinforcement without significantly degrading the other properties of hydrogels. Assays of transmission electron microscopy, 1 H nuclear magnetic resonance, and dynamic light scattering reveal that EOA can spontaneously and quickly self-assemble into robust nanoparticles in water, and this nanoparticle structure can be kept at pH 3~9. Measurement of the gel time by rheometer and the stir bar method confirms the formation of the eGels, and their gel time is dependent on the weight content of nEOAs. As expected, adding nEOAs to hydrogels does not cause the phase separation (scanning electron microscopy observation), but it improves mechanical strength up to ~8 kPa and conductivity up to ~10 −6 S/cm in our studied range. Incubating eGels in phosphate-buffered saline leads to their further swelling with an increase of water content <6% and gradual degradation. When growing mesenchymal stem cells on eGels with nEOA content ≤14%, the growth curves and morphology of cells were found to be similar to that on tissue culture plastic; when implanting these eGels on a chick chorioallantoic membrane for 1 week, mild inflammation response appeared without any other structural changes, indicating their good in vitro and in vivo biocompatibility. With injectability, uniformity, degradability, electroactivity, relative robustness, and biocompatibility, these eGels may have a huge potential as scaffolds for tissue regeneration and matrix for stimuli responsive drug release.

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Soft tissue adhesive composed of modified gelatin and polysaccharides

Cited by 98 publications

References 16 publications

A biodegradable and biocompatible gecko-inspired tissue adhesive

A biodegradable and biocompatible gecko-inspired tissue adhesive

The relative contribution of calcium, zinc and oxidation-based cross-links to the stiffness ofArion subfuscusglue

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

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