Soft Polyethylene Glycol Hydrogels Support Human PSC Pluripotency and Morphogenesis

Seitz, Michael P.; Song, Yuanhui; Lian, Xiaojun Lance; Ma, Zhen; Jain, Era

doi:10.1021/acsbiomaterials.4c00923

Cited by 2 publications

(1 citation statement)

References 74 publications

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“…In addition, AB-PAS staining indicated that the cell aggregates contained mucus. As was reported that the elasticity of biomaterial platforms plays important role on cell differentiation, − the findings of this study suggest that the presence of 15% PEG10000 in the cell culture medium is conducive to an appropriate elasticity required for the development of STOs.…”

Section: Resultsmentioning

confidence: 51%

Forming Single-Cell-Derived Colon Cancer Organoid Arrays on a Microfluidic Chip for High Throughput Tumor Heterogeneity Analysis

Chen,

Lin

et al. 2024

ACS Biomater. Sci. Eng.

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Single-cell-derived tumor organoids (STOs) possess a distinct genetic background, making them valuable tools for demonstrating tumor heterogeneity. In order to fulfill the high throughput demands of STO assays, we have developed a microfluidic chip containing 30 000 microwells, which is dedicated to a single cell culture approach for selective expansion and differential induction of cancer stem cells. The microwells are coated with a hydrophilic copolymer to eliminate cell adhesion, and the cell culture is supported by poly(ethylene glycol) (PEG) to establish a nonadhesive culture environment. By utilizing an input cell density of 7 × 10 3 •mL −1 , it is possible to construct a 4000 single cell culture system through stochastic cell occupation. We demonstrate that the addition of 15% PEG10000 in the cell culture medium effectively prevents cell loss while facilitating tumor stem cell expansion. As were demonstrated by HCT116, HT29, and SW480 colon cancer cells, the microfluidic approach achieved a STO formation rate of ∼20%, resulting in over 800 STOs generated from a single culture. Comprehensive analysis through histomorphology, immunohistochemistry, drug response evaluation, assessment of cell invasion, and biomarker detection reveals the heterogeneity among individual STOs. Specifically, the smaller STOs exhibited higher invasion and drug resistance capabilities compared with the larger ones. The developed microfluidic approach effectively facilitates STO formation and offers promising prospects for investigating tumor heterogeneity, as well as conducting personalized therapy-focused drug screening.

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Section: Resultsmentioning

confidence: 51%

Forming Single-Cell-Derived Colon Cancer Organoid Arrays on a Microfluidic Chip for High Throughput Tumor Heterogeneity Analysis

Chen,

Lin

et al. 2024

ACS Biomater. Sci. Eng.

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Biomaterials Mimicking Mechanobiology: A Specific Design for a Specific Biological Application

Donati,

Valicenti,

Giannoni

et al. 2024

IJMS

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Mechanosensing and mechanotransduction pathways between the Extracellular Matrix (ECM) and cells form the essential crosstalk that regulates cell homeostasis, tissue development, morphology, maintenance, and function. Understanding these mechanisms involves creating an appropriate cell support that elicits signals to guide cellular functions. In this context, polymers can serve as ideal molecules for producing biomaterials designed to mimic the characteristics of the ECM, thereby triggering responsive mechanisms that closely resemble those induced by a natural physiological system. The generated specific stimuli depend on the different natural or synthetic origins of the polymers, the chemical composition, the assembly structure, and the physical and surface properties of biomaterials. This review discusses the most widely used polymers and their customization to develop biomaterials with tailored properties. It examines how the characteristics of biomaterials-based polymers can be harnessed to replicate the functions of biological cells, making them suitable for biomedical and biotechnological applications.

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Soft Polyethylene Glycol Hydrogels Support Human PSC Pluripotency and Morphogenesis

Cited by 2 publications

References 74 publications

Forming Single-Cell-Derived Colon Cancer Organoid Arrays on a Microfluidic Chip for High Throughput Tumor Heterogeneity Analysis

Forming Single-Cell-Derived Colon Cancer Organoid Arrays on a Microfluidic Chip for High Throughput Tumor Heterogeneity Analysis

Biomaterials Mimicking Mechanobiology: A Specific Design for a Specific Biological Application

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