Soft chitosan microbeads scaffold for 3D functional neuronal networks

Tedesco, Mariateresa; Lisa, Donatella Di; Massobrio, Paolo; Colistra, Nicolò; Pesce, Mattia; Catelani, Tiziano; Dellacasa, Elena; Raiteri, Roberto; Martinoia, Sergio; Pastorino, Laura

doi:10.1016/j.biomaterials.2017.11.043

Cited by 69 publications

(59 citation statements)

References 78 publications

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“…Previous studies have demonstrated that chitosan can enhance neuron adhesion, proliferation and neurite outgrowth. Chitosan microbead‐based scaffolds coupled with microelectrode arrays (MEAs) were fabricated to engineer 3D hippocampal neural networks with electrophysiological properties . In another case, a dual hydrogel‐based 3D peripheral nerve‐on‐a‐chip was established to microengineer sensory neural fiber tract that resembled afferent sensory peripheral fibers in vivo .…”

Section: Hydrogels In Organs‐on‐a‐chip Engineeringmentioning

confidence: 99%

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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The major motivation is to better mimic human physiology and functions at multiscales from the molecular to the cellular, tissue, organ or even whole organism level. Current model systems primarily rely on the monolayer cell cultures and animal models. Simplistic monolayer cultures have their advantages, but they are often significantly different in gene expression, epigenetics, and cell function compared to native 3D tissues. [1,2] Also, they often lack cell-cell and cell-matrix interactions, [2,3] leading to the absence of tissue specific properties. Although animal models are widely used in biomedical research, they fail to faithfully predict human responses in a physiologically relevant manner due to the significant species divergences. [4] The limitations of these systems have provided an impetus for the development of alternative cell-based 3D models in vitro that better resemble the complex functionalities of living organs. Over the last several years, organoids and organ-on-a-chip (OOC), representing the major technological breakthrough, have emerged as two distinct model systems to achieve the same goal of building 3D organotypic models in vitro by bridging the gap between animal models and monolayer cultures. These engineered models are different in terms of cell source, tissue composition, architectural variability, functional features, scales, cellular fidelity, etc. Organoids, primarily evolving from developmental principles, refer to 3D multicellular tissues by self-organization of stem cells or organ-specific progenitors, which can recapitulate the intricate architectures and functionalities of in vivo organs. [5][6][7] Recent breakthroughs in organoid technology have enabled the successful generation of a variety of human organoids, such as the brain, [8,9] intestine, [10,11] liver, [12] kidney, [13,14] lung, [15] etc. These near physiological 3D organoids have garnered momentum for their potential applications in human organ development and disease modeling, drug screening and regenerative medicine. The explosion of organoids research has been catalyzed by the significant progress of stem cell biology and availability of human stem cells. In contrast, the OOC, relying on the bioengineering design principle, is a miniaturized in vitro model that can recreate the functional units of living organs on a microfluidic cell culture device using predifferentiated cells, often cell lines. [1,16] The OOC is primarily evolved from the convergence of tissue engineering and microfabricated technologies, which is characterized by the capability to simulate cellular microenvironment by precise control over fluid flow, mechanical forces and biochemical factors. [4,17] Remarkable progress has been made Significant advances in materials, microscale technology, and stem cell biology have enabled the construction of 3D tissues and organs, which will ultimately lead to more effective diagnostics and therapy. Organoids and organs-on-a-chip (OOC), evolved from developmental biology and bioengineering principles, have e...

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Section: Hydrogels In Organs‐on‐a‐chip Engineeringmentioning

confidence: 99%

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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“…Chitosan-based microspheres were also applied as microcarriers for cell culture [75,[78][79][80], because of the non-toxicity and good biodegradability of chitosan [81,82]. Zhang et al [75] developed hybrid microspheres from chitosan and graphene oxide, which supported stem cell expansion, growth, and proliferation.…”

Section: Non-porous Microspheresmentioning

confidence: 99%

Biopolymer-Based Microcarriers for Three-Dimensional Cell Culture and Engineered Tissue Formation

Huang

Abdalla

Xiao

et al. 2020

IJMS

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The concept of three-dimensional (3D) cell culture has been proposed to maintain cellular morphology and function as in vivo. Among different approaches for 3D cell culture, microcarrier technology provides a promising tool for cell adhesion, proliferation, and cellular interactions in 3D space mimicking the in vivo microenvironment. In particular, microcarriers based on biopolymers have been widely investigated because of their superior biocompatibility and biodegradability. Moreover, through bottom-up assembly, microcarriers have opened a bright door for fabricating engineered tissues, which is one of the cutting-edge topics in tissue engineering and regeneration medicine. This review takes an in-depth look into the recent advancements of microcarriers based on biopolymers—especially polysaccharides such as chitosan, chitin, cellulose, hyaluronic acid, alginate, and laminarin—for 3D cell culture and the fabrication of engineered tissues based on them. The current limitations and potential strategies were also discussed to shed some light on future directions.

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“…Thus, various scaffolds based on different materials have been developed for culturing cells into spheroids (Figure 1a). [58][59][60][61] Among them, natural extracellular matrix-derived hydrogels are most widely used in in vitro 3D cell culture applications. Hydrogels can be used in 3D culture systems in a variety of ways: by coating the surface of a solid scaffold, or by wrapping or sandwiching the cells in the middle of the matrix.…”

Section: Scaffold-based Culture Systemmentioning

confidence: 99%

Development of Cell Spheroids by Advanced Technologies

Shao

Chi

Zhang

et al. 2020

Adv Materials Technologies

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3D cell culture has been strongly advocated as a highly useful culture technique instead of the traditional 2D cell culture. Specially, spheroids, as the primary mode of 3D cell culture, allow cells to establish a connection between the cell and the extracellular matrix to form a specific 3D structure that better mimics the growing environment of cells in vivo. Benefiting from the emergence of various advanced micromachining platforms, spheroids have received increasing attention in diverse fields. Herein, the combination of the cell spheroid culture with microfluidic technology is reviewed. After concisely introducing the traditional methods for fabricating cell spheroids, an outline of the approaches for preparing cell spheroids using different advanced techniques is given. Then, the various applications of the generated cell spheroids in the field of biomedical engineering are presented and the current limitations, challenges, and future directions are summarized.

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Soft chitosan microbeads scaffold for 3D functional neuronal networks

Cited by 69 publications

References 78 publications

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Biopolymer-Based Microcarriers for Three-Dimensional Cell Culture and Engineered Tissue Formation

Development of Cell Spheroids by Advanced Technologies

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