Sodium transport by endocytic vesicles in cultured Arabidopsis thaliana (L.) Heynh. cells

Orlova, Yu. V.; Сергиенко, О. В.; Халилова, Л. А.; Воронков, А. С.; Фоменков, А. А.; Носов, А. В.; Попова, Л. Г.; Shuvalov, Alexey; Ryabova, Anastasia V.; Balnokin, Yu. V.

doi:10.1007/s11627-019-10005-7

Cited by 9 publications

(6 citation statements)

References 47 publications

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“…However, 3 genes encoding proteins of the vesicle secretion system were in this group (Protein ID_74241, a homolog to Sec66; Protein ID_73021, a Synaptobrevin homolog; and Protein ID_73943, Syntaxin VAM3), being markedly induced Sec66 which reached a fold change of 11.8, the highest level on induction among all. Interestingly, their induction would support the involvement of the vesicle secretion system in the movement of NaCl in cells previously described in plants, which would exist as a parallel route for the transport of Na + from the outside to be discharged into the vacuole by endocytosis and, in turn, could remove Na + from the cell to the outside by exocytosis ( Leshem et al, 2006 ; Baral et al, 2015 ; Garcia de la Garma et al, 2015 ; Flowers et al, 2019 ; Orlova et al, 2019 ). Another membrane protein that was significantly induced in response to salt was Protein ID_73143, an homolog to PMP3 of S. cerevisiae and RCI2A from plants, described as a regulator of membrane potential under saline conditions ( Navarre and Goffeau, 2000 ; Nylander et al, 2001 ).…”

Section: Resultssupporting

confidence: 61%

The transportome of the endophyte Serendipita indica in free life and symbiosis with Arabidopsis and its expression in moderate salinity

Haro,

Lanza,

Aguilella

et al. 2023

Front. Microbiol.

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Serendipita indica is an endophytic root symbiont fungus that enhances the growth of various plants under different stress conditions, including salinity. Here, the functional characterization of two fungal Na+/H+ antiporters, SiNHA1 and SiNHX1 has been carried out to study their putative role in saline tolerance. Although their gene expression does not respond specifically to saline conditions, they could contribute, together with the previously characterized Na+ efflux systems SiENA1 and SiENA5, to relieve Na+ from the S. indica cytosol under this stressed condition. In parallel, an in-silico study has been carried out to define its complete transportome. To further investigate the repertoire of transporters expressed in free-living cells of S. indica and during plant infection under saline conditions, a comprehensive RNA-seq approach was taken. Interestingly, SiENA5 was the only gene significantly induced under free-living conditions in response to moderate salinity at all the tested time points, revealing that it is one of the main salt-responsive genes of S. indica. In addition, the symbiosis with Arabidopsis thaliana also induced SiENA5 gene expression, but significant changes were only detected after long periods of infection, indicating that the association with the plant somehow buffers and protects the fungus against the external stress. Moreover, the significant and strongest induction of the homologous gene SiENA1 occurred during symbiosis, regardless the exposure to salinity. The obtained results suggest a novel and relevant role of these two proteins during the establishment and maintenance of fungus-plant interaction.

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Section: Resultssupporting

confidence: 61%

The transportome of the endophyte Serendipita indica in free life and symbiosis with Arabidopsis and its expression in moderate salinity

Haro,

Lanza,

Aguilella

et al. 2023

Front. Microbiol.

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“…To mitigate the consequences produced by salt stress at the physiological, cellular and molecular level, plants respond by activating different mechanisms. A high concentration of Na + ions will cause an ionic imbalance, triggering a massive entry of toxic ions into the cells and will also produce changes at the lipid level ( Baral et al., 2015b ; Orlova et al., 2019 ). Along with this, salinity stimulates the accumulation of ROS, which in high concentrations are toxic to cells.…”

Section: Discussionmentioning

confidence: 99%

A SNARE-like protein from Solanum lycopersicum increases salt tolerance by modulating vesicular trafficking in tomato

et al. 2023

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Intracellular vesicular trafficking ensures the exchange of lipids and proteins between endomembrane compartments. This is relevant under high salinity conditions, since both the removal of transporters and ion channels from the plasma membrane and the compartmentalization of toxic ions require the formation of vesicles, which can be maintained as multivesicular bodies or be fused to the central vacuole. SNARE proteins (Soluble N-ethylmaleimide-sensitive factor attachment receptor) participate in the vesicle fusion process and give specificity to their destination. Plant genome studies have revealed a superfamily of genes that encode for proteins called SNARE-like. These proteins appear to be participating in vesicular trafficking with similar functions to those of SNARE proteins. A SNARE-like, named SlSLSP6, in Solanum lycopersicum plants has been shown to be induced under high salinity conditions. A phylogenetic relationship of SlSLSP6 with SNARE-like proteins of salinity-tolerant plants, including Salicornia brachiata, Zostera marina and Solanum pennelli, was determined. Considering its amino acid sequence, a putative clathrin adapter complex domain and palmitoylation site was predicted. Subcellular localization analysis evidenced that SlSLSP6 is mostly localized in the plasma membrane. Using transgenic tomato plants, we identified that overexpression of SlSLSP6 increased tolerance to salt stress. This tolerance was evident when we quantified an improvement in physiological and biochemical parameters, such as higher chlorophyll content, performance index, efficiency of photosystem II and relative water content, and lower malondialdehyde content, compared to control plants. At the subcellular level, the overexpression of SlSLSP6 reduced the presence of H2O2 in roots and increased the compartmentalization of sodium in vacuoles during salt stress. These effects appear to be associated with the higher endocytic rate of FM4-64, determined in the plant root cells. Taken together, these results indicate that SlSLSP6 increases tolerance to salt stress by modulating vesicular trafficking through over-induction of the endocytic pathway. This work contributes to understanding the role of this type of SNARE-like protein during salt stress and could be a potential candidate in breeding programs for tolerance to salt stress in tomato plants.

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“…However, Rab-GTPs have diverse functions and the specific mechanism for enhanced Na + relocation may vary. For example, Rab-directed trafficking of Na +containing vesicles can occur from the plasma membrane to the vacuole in Arabidopsis cell cultures [62]. In addition, Rab-GTPs are involved in fusion of endosomes, pre-vacuole compartments and vacuoles in yeast and mammalian cells [63,64], which could increase storage capacity as proposed for Arabidopsis AtRabG overexpression lines [51].…”

Section: Discussionmentioning

confidence: 99%

Salt-induced expression of intracellular vesicle trafficking genes, CaRab-GTP, and their association with Na+ accumulation in leaves of chickpea (Cicer arietinum L.)

et al. 2020

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Background Chickpea is an important legume and is moderately tolerant to salinity stress during the growing season. However, the level and mechanisms for salinity tolerance can vary among accessions and cultivars. A large family of CaRab-GTP genes, previously identified in chickpea, is homologous to intracellular vesicle trafficking superfamily genes that play essential roles in response to salinity stress in plants. Results To determine which of the gene family members are involved in the chickpea salt response, plants from six selected chickpea accessions (Genesis 836, Hattrick, ICC12726, Rupali, Slasher and Yubileiny) were exposed to salinity stress and expression profiles resolved for the major CaRab-GTP gene clades after 5, 9 and 15 days of salt exposure. Gene clade expression profiles (using degenerate primers targeting all members of each clade) were tested for their relationship to salinity tolerance measures, namely plant biomass and Na+ accumulation. Transcripts representing 11 out of the 13 CaRab clades could be detected by RT-PCR, but only six (CaRabA2, −B, −C, −D, −E and −H) could be quantified using qRT-PCR due to low expression levels or poor amplification efficiency of the degenerate primers for clades containing several gene members. Expression profiles of three gene clades, CaRabB, −D and −E, were very similar across all six chickpea accessions, showing a strongly coordinated network. Salt-induced enhancement of CaRabA2 expression at 15 days showed a very strong positive correlation (R2 = 0.905) with Na+ accumulation in leaves. However, salinity tolerance estimated as relative plant biomass production compared to controls, did not correlate with Na+ accumulation in leaves, nor with expression profiles of any of the investigated CaRab-GTP genes. Conclusion A coordinated network of CaRab-GTP genes, which are likely involved in intracellular trafficking, are important for the salinity stress response of chickpea plants.

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Sodium transport by endocytic vesicles in cultured Arabidopsis thaliana (L.) Heynh. cells

Cited by 9 publications

References 47 publications

The transportome of the endophyte Serendipita indica in free life and symbiosis with Arabidopsis and its expression in moderate salinity

The transportome of the endophyte Serendipita indica in free life and symbiosis with Arabidopsis and its expression in moderate salinity

A SNARE-like protein from Solanum lycopersicum increases salt tolerance by modulating vesicular trafficking in tomato

Salt-induced expression of intracellular vesicle trafficking genes, CaRab-GTP, and their association with Na+ accumulation in leaves of chickpea (Cicer arietinum L.)

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