Sodium-sensitive and -insensitive copper accumulation by isolated intestinal cells of rainbow trout<i>Oncorhynchus mykiss</i>

Burke, J.; Handy, Richard D.

doi:10.1242/jeb.01379

Cited by 39 publications

(13 citation statements)

References 77 publications

(137 reference statements)

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“…Since intestinal uptake of copper predominates in fish (Kamunde et al, 2002;Kamunde et al, 2001) this suggests a role for Ctr1 in uptake of dietary copper in fish, as in mammals. Indeed previous studies on Cu uptake in fish intestine provides evidence for the presence of a transporter with similar kinetics (high affinity/low capacity) to that of Ctr1 Burke and Handy, 2005) and our finding that Ctr1 mRNA is most highly expressed in intestine supports this contention.…”

Section: Discussionsupporting

confidence: 87%

“…Even so, making use of the available data, the results presented here are consistent with a role for a high affinity carrier such as Ctr1 in regulating excess and normal copper uptake. Thus, Cu-uptake exhibits kinetics in isolated rainbow trout (Oncorhynchus mykiss), consistent with the presence of Ctr1-like activity (Burke and Handy, 2005) and rainbow trout acclimated to Cu in the diet show increased Cu tolerance to both waterborne and subsequent dietary exposure (Miller et al, 1993). In another freshwater species, the catfish, Clarias gariepinus, Cu uptake across the gut has also been shown to be negatively regulated in isolated, perfused intestine, Cu uptake efficiency declining with increasing luminal Cu concentration (Handy et al, 2000).…”

Section: Discussionsupporting

confidence: 61%

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Copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (Sparus aurata) after exposure to dietary or waterborne copper

Minghetti

Leaver

Carpenè

et al. 2008

Comparative Biochemistry and Physiology Part C: Toxicology &

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The high affinity copper transporter 1 (Ctr1), metallothionein (MT) and glutathione reductase (GR) are essential for copper uptake, sequestration and defense respectively. Cu-exposure increased Ctr1 mRNA levels in the intestine and the kidney compared to experimental controls. Excess dietary Cu exposure had no effect on levels of metallothionein (MT) mRNA, and the only effect of dietary excess Cu on glutathione reductase (GR) mRNA was a decrease in the intestine. Both MT mRNA and GR were increased in the liver and gill after waterborne Cu exposure, compared to levels in fish fed experimental control low Cu diets. Thus, Ctr1, MT and GR mRNA expression in response to excess Cu is dependent on the route of exposure. Furthermore, the tissue expression profile of sea bream Ctr1 is consistent with the known physiology of copper exposure in fish and indicates a role both in essential copper uptake and in avoidance of excess dietary and waterborne copper influx.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 61%

Copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (Sparus aurata) after exposure to dietary or waterborne copper

Minghetti

Leaver

Carpenè

et al. 2008

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Marine gulf toadfish, Opsanus beta , exposed to 761 µg/L for both 96 h and 30 d had increased plasma sodium, chloride, and osmolality followed by fluid loss in muscle tissue as well as disturbance of chloride absorption in the intestine (Grosell et al 2004). Therefore, Cu uptake across the gut must be carefully regulated in fish (Bury et al 2003; Burke and Handy 2005).…”

mentioning

confidence: 99%

Evaluation of Toxicity of Dietary Chelated Copper in Juvenile Olive Flounder, Paralichthys olivaceus, Based on Growth and Tissue Copper Concentration

Mohseni¹,

Park²,

Lee³

et al. 2012

J World Aquaculture Soc

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The present study was conducted to determine the safe and toxic levels of dietary copper in juvenile olive flounder, Paralichthys olivaceus, fed Mintrex® copper, a chelated dietary copper source. Fish averaging 3.8 ± 0.13 g (mean ± SD) were fed 1 of 10 diets (n = 3) containing 7 (Cu0), 10.4 (CuM5), 15.8 (CuM10), 24.9 (CuM20), 43.4 (CuM40), 82.1 (CuM80), 158 (CuM160), 308 (CuM320), 658 (CuM640), and 1267 (CuM1280) mg Cu/kg diet. At the end of 12 wk of feeding trial, weight gain (WG), specific growth rate, and protein efficiency ratio of fish fed CuM5 and CuM10 diets were significantly higher than those fed CuM80, CuM160, CuM320, CuM640, and CuM1280 diets (P < 0.05). Survival of fish fed Cu0, CuM5, CuM10, CuM20, and CuM40 diets was significantly higher than those of fish fed CuM320, CuM640, and CuM1280 diets. Whole‐body lipid content of fish decreased while whole‐body ash increased with dietary copper levels. Whole‐body and tissue copper concentrations increased with dietary copper levels. Although ANOVA test suggested that the toxic level of dietary Cu in juvenile olive flounder, P. olivaceus, could be 320 mg/kg diet, broken‐line analysis of WG indicated a level of 286 mg/kg diet when Mintrex®Cu is used as the dietary copper source.

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“…The Ca 2+ content was immediately measured using Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) Varian 725-ES-2007 (Australia) as described by Burke & Handy (2005). Afterwards, 1 9 10 6 cells were lysed using 2 mL nitric acid (0.1 M) in a fresh polypropylene tube and incubated for 1 h at 70°C.…”

Section: Measurement Of Ins-1 Intracellular Ca 2+ Concentrationmentioning

confidence: 99%

“…Afterwards, 1 9 10 6 cells were lysed using 2 mL nitric acid (0.1 M) in a fresh polypropylene tube and incubated for 1 h at 70°C. The Ca 2+ content was immediately measured using Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) Varian 725-ES-2007 (Australia) as described by Burke & Handy (2005).…”

Section: Measurement Of Ins-1 Intracellular Ca 2+ Concentrationmentioning

confidence: 99%

Effects of the microbial secondary metabolites pyrrolnitrin, phenazine and patulin on INS-1 rat pancreatic β-cells

Nisr

Russell

Chrachri

et al. 2011

FEMS Immunol Med Microbiol

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The effects on pancreatic β-cell viability and function of three microbial secondary metabolites pyrrolnitrin, phenazine and patulin were investigated, using the rat clonal pancreatic β-cell line, INS-1. Cells were exposed to 10-fold serial dilutions (range 0-10 μg mL(-1)) of the purified compounds for 2, 24 and 72 h. After 2 h exposure, only patulin (10 μg mL(-1)) was cytotoxic. All compounds showed significant cytotoxicity after 24 h. None of the compounds altered insulin secretion with 2 and 20 mM glucose after 2 h. However, after 24 h treatment, phenazine and pyrrolnitrin (10 and 100 ng mL(-1)) potentiated insulin production and glucose-stimulated insulin secretion, whereas patulin had no effect. Exposure (24 h) to either phenazine (100 ng mL(-1)) or pyrrolnitrin (10 ng mL(-1)) caused similar increases in the Ca(2+) content of INS-1 cells. The outward membrane current was inhibited after 24 h exposure to either phenazine (100 ng mL(-1)) or pyrrolnitrin (10 or 100 ng mL(-1)). This study presents novel data suggesting that high concentrations of pyrrolnitrin and phenazine are cytotoxic to pancreatic β-cells and thus possibly diabetogenic, whereas at lower concentrations these agents are nontoxic and may be insulinotropic. The possible role of such agents in the development of cystic fibrosis-related diabetes is discussed.

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Sodium-sensitive and -insensitive copper accumulation by isolated intestinal cells of rainbow troutOncorhynchus mykiss

Cited by 39 publications

References 77 publications

Copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (Sparus aurata) after exposure to dietary or waterborne copper

Copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (Sparus aurata) after exposure to dietary or waterborne copper

Evaluation of Toxicity of Dietary Chelated Copper in Juvenile Olive Flounder, Paralichthys olivaceus, Based on Growth and Tissue Copper Concentration

Effects of the microbial secondary metabolites pyrrolnitrin, phenazine and patulin on INS-1 rat pancreatic β-cells

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