Sodium Retaining Activity of 19-Nor-Steroids in Adrenalectomized Rats

Kagawa, C. M.; Arman, C. G. Van

doi:10.3181/00379727-94-22972

Cited by 35 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bilateral adrenal compression (2) and castration of 50 male (100-120 g) Sprague-Dawley rats (Taconic Farms) was done 19-NOR-DOC IN ADRENAL REGENERATION HYPERTENSION 709 under ether anesthesia. Both kidneys were left intact to prevent the development of malignant hypertension.…”

Section: Methodsmentioning

confidence: 99%

19-Nor-Deoxycorticosterone: A Potent Mineralcocorticoid Isolated from the Urine of Rats with Regenerating Adrenals*

Gómez-Sánchez

Holland²,

Murry

et al. 1979

Endocrinology

View full text Add to dashboard Cite

Adrenal regeneration hypertension (ARH) is an experimental form of low renin hypertension in the rat in which an unknown mineralocorticoid (s) appears to contribute to the pathophysiology of the hypertension. To isolate unknown mineralocorticoids, urine of castrated rats with regenerating adrenals was lowered to pH 1.0 with sulfuric acid, incubated for 24-h at room temperature, extracted with ethyl acetate, and subjected to several successive chromatographic purifications. Unknown urinary mineralocorticoid activity was identified and monitored with an aldosterone radioreceptor assay to enable isolation of approximately 1 mg of a pure unknown steroid. Direct probe mass spectrometry, gas chromatography-mass spectrometry, and nuclear magnetic resonance revealed the following characteristics of the isolated steroid: 1) a molecular weight of 316; 2) a deficit in 14 atomic mass units in comparison to deoxycorticosterone (DOC); 21-hydroxy-4-pregnene-3,20-dione) and to the corresponding dimethoxime and dimethoxime-trimethylsilyl derivatives of the unknown steroid and of DOC; and (3) nuclear magnetic resonance spectrum identical to that of DOC except for the absence of the angular C-10 methyl signal. Further characterization revealed circular dichroism of the unknown steroid almost identical to that of DOC; UV absorption maximum at 239 nm, suggesting a 4-en-3-oxo function; and positive blue tetrazolium reaction, indicating a a-ketol. These data suggested that the steroid was 19-nor-DOC. Mass spectrometry of the isolated steroid, authentic 19-nor-DOC, and their dimethoxime-trimethylsilyl derivatives substantiated this conclusion. This is the first reported identification of 19-nor-DOC as a naturally occurring steroid. However, previous bioassay data have established the mineralocorticoid potency of 19-nor-DOC to be 1.5-5.1 times that of DOC. The role of this newly isolated mineralocorticoid in adrenal regeneration hypertension and human hypertension remains to be elucidated. {Endocrinology 105: 708, 1979)

show abstract

Section: Methodsmentioning

confidence: 99%

19-Nor-Deoxycorticosterone: A Potent Mineralcocorticoid Isolated from the Urine of Rats with Regenerating Adrenals*

Gómez-Sánchez

Holland²,

Murry

et al. 1979

Endocrinology

View full text Add to dashboard Cite

show abstract

“…Since 19-nor-DOC is a potent mineralocorticoid (2), it could prove to be important in the etiology of some hypertensive diseases (3). It has been suggested that this steroid originates from an extraadrenal site (4,5) so that any one of three precursors, 19-hydroxy-DOC, 19-oxo-DOC, or 19-oic-DOC, synthesized in the adrenal, may likewise be of physiological significance.…”

mentioning

confidence: 99%

Further Studies of the Mineralocorticoid Activity of 19-oxo-Deoxycorticosterone*

1984

View full text Add to dashboard Cite

Antinatriuretic and kaliuretic activities of 19-oxo-deoxycorticosterone (19-oxo-DOC) were measured in male adrenalectomized rats and compared with those of aldosterone. No significant effects of 19-oxo-DOC or aldosterone were observed in the lag period (i.e. the first hour post injection). In the subsequent 2 h, rats injected with 25 micrograms 19-oxo-DOC excreted less than half the sodium and more than twice the potassium compared to rats injected with vehicle alone. Overall mineralocorticoid activity (based on changes in urinary Na+/K+), antinatriuretic activity (based on changes in urinary Na+/creatinine) and kaliuretic activity (based on changes in urinary K+/creatinine) of 19-oxo-DOC were all in the range of 1:100th-1:200th that of aldosterone. These results are not in agreement with a recent report suggesting that 19-oxo-DOC possesses antinatriuretic activity but no kaliuretic activity.

show abstract

“…It is known that 19-nor-DOC is a potent mineralocorticosteroid (1)(2)(3)(4)(5)(6) that is present in nonconjugated form in urine of rats undergoing adrenal regeneration (5) and in rats with spontaneous (7) hypertension as well as in urine of normotensive animals. The levels of 19-nor-DOC in urine of these hypertensive rats appear to be greater than those in normotensive rats.…”

Section: Introductionmentioning

confidence: 99%

Origin of urinary nonconjugated 19-nor-deoxycorticosterone and metabolism of infused radiolabeled 19-nor-deoxycorticosterone in men and women.

Casey¹,

Guerami²,

Milewich³

et al. 1985

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

Sodium Retaining Activity of 19-Nor-Steroids in Adrenalectomized Rats

Cited by 35 publications

References 0 publications

19-Nor-Deoxycorticosterone: A Potent Mineralcocorticoid Isolated from the Urine of Rats with Regenerating Adrenals*

19-Nor-Deoxycorticosterone: A Potent Mineralcocorticoid Isolated from the Urine of Rats with Regenerating Adrenals*

Further Studies of the Mineralocorticoid Activity of 19-oxo-Deoxycorticosterone*

Origin of urinary nonconjugated 19-nor-deoxycorticosterone and metabolism of infused radiolabeled 19-nor-deoxycorticosterone in men and women.

Contact Info

Product

Resources

About