Sodium, Potassium, Calcium and Copper Levels in Seminal Plasma are Associated with Sperm Quality in Fertile and Infertile Men

Hamad, Abdul-Wahab R.; Al-Daghistani, Hala I.; Shquirat, Walid D.; Abdel-Dayem, Muna; Al-Swaifi, Mohammad

doi:10.4172/2167-0501.1000141

Cited by 18 publications

(18 citation statements)

References 35 publications

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“…The flame emission intensity was considered proportional to the concentration of sodium and potassium in the milt. Concentrations were determined by comparison with standard curves covering different concentration ranges (Hamad et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of extenders for refrigerated preservation ofLabeo rohitamilt

et al. 2021

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The present study was designed to determine physico‐chemical characteristics and best extender for refrigerated preservation of Labeo rohita milt. In experiment I, milt was collected from mature and healthy brooders (n = 5) of L. rohita reared at fish seed hatchery, Rawal Town, Islamabad, Pakistan. After collection, milt was maintained at 4°C and physico‐chemical characteristics were studied. The mean volume of Labeo rohita milt was 8.02 ± 0.12 ml, pH 7.60 ± 0.17, sperm count 2.77 ± 0.19 1010/ml, osmolality 267.6 ± 7.4 mOsmol/kg, sodium 95.43 ± 6.7 (mM/L), potassium 34.41 ± 1.2 (mM/L), total lipids 178.94 ± 5.0 mg/dl and motility 83.5 ± 5.0%. In experiment II, nine male brooders (3 brooders/replicate) maintained in three different ponds were used. In each replicate, milt from three brooders was pooled, kept at 4°C and divided into 4 aliquots. An aliquot was kept undiluted (control), while other aliquots were diluted in Alsever's solution, sugar‐based extender and modified Kurokura extender (KE) in 1:9 ratio at 4°C and stored up to 7 days. Sperm motility and viability were assessed for up to 7 days. Total antioxidant capacity was measured on Day 4. Sperm motility was higher (p < 0.05) in milt diluted with KE compared with control and other diluents from Day 5 to Day 7. However, highest sperm viability was observed on Day 6 and Day 7 in sperm diluted with KE. Total antioxidant capacity of milt diluted with KE and control was higher than other diluents on Day 4. In conclusion, viability of Labeo rohita sperm can be increased by diluting it in KE.

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Section: Methodsmentioning

confidence: 99%

Evaluation of extenders for refrigerated preservation ofLabeo rohitamilt

et al. 2021

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“…Despite, E4 contained Na + cation comes from sodium chloride, but saline solution in E5 extender had adjusted during industry that led to goodness chilling preservation. In addition, the highest value of Na + cation presented in saline solution (E5 extender) plus other cations such as, K + , Ca ++ and Mg ++ in the seminal plasma may play essential roles in the sperm activity and establish the osmotic balance (Hamad et al, 2014). On the other hand, (Zeny, 2016) indicated that Na + generally plays an important role in the activation sperm motility which may be an indication of sperm plasma membrane integrity and Na + cation exhibited favorable effects on the seminal quality, antioxidant balance and positive impact on all spermatozoa vitality characteristics.…”

Section: Sperm Motility (Spm)mentioning

confidence: 99%

Impact of Conventional and Non-Conventional Extenders on Rams Semen Quality During Storage at 5ºC

Khalifa

Khalil

2016

Journal of Animal and Poultry Production

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The aim of the present study was to define the effect of different extender components (as conventional and nonconventional extenders) on rams' semen quality during storage at 5ºC for up to 6 days. Fertility rates of the extended ram spermatozoa stored at 5ºC for up one day were also studied. Four Rahmani rams were used to collect pooled ejaculates by artificial vagina. Semen was collected, evaluated and extended with five extenders included conventional and non-conventional extender ingredients. The conventional extenders were sodium citrate (E1), Tris (E2) and skimmed milk powder (E3)-based glucose-egg yolk. While, non-conventional extenders were salt of sodium chloride 0.9% (E4) and saline solution 0.9% intravenous infusion (E5) based glucose-egg yolk. The final extension rate was 1semen : 6 extender. The extended semen with different extenders media (E1, E2, E3, E4 and E5) were cooled to 5ºC and stored at this temperature for 6 days. After storage time (0, 1, 2, 3, 4, 5 and 6 days), the percentage of sperm motility (SPM), recovery sperm motility (RSM) and positive osmotic resistant (POR) were recorded. Also, fertility rate was carried out with liquid semen stored at 5ºC for up to one day using the best conventional and non-conventional extenders. Thus, twenty-two ewes were used to investigate fertility rate. Ewes were divided into two groups (11/group), the1 st and 2 nd groups were artificially inseminated with conventional (E2) or non-conventional (E5) extenders which obtained better semen quality than other extenders, respectively. The results indicated that, the cooling extended ram semen with each of E1, E2 or E5 extenders was significantly (P<0.05) higher the percentage of SPM, RSM and POR than those extended with E3 and E4 extenders during storage at 5ºC for up to 6 days. However, the cooling ram semen with E2 and E5 showed non-significantly parameters during cooled at 5ºC till 6 days. The statistical analysis of the data revealed a significant positive correlation between extender types and SPM, RSM and POR of ram spermatozoa during storage at 5ºC until 6 days. Surprisingly, fertility rate of ewes artificially inseminated with ram semen preserved at 5ºC for one day of storage performed to assess higher conception rate (84.62%) with E5 (as non-conventional extender) than conception rate (78.57%) with E2 (as conventional extender). Moreover, the superior litter size recorded 1.00 with ram semen extended with E5 compared to 0.86 with E2 extenders. Also, sex ratio of born lambs displayed female lambs 66.67 and 33.33% of male lambs with semen extended with E2 extender. Ram semen extended with E5 achieved female lambs 69.23% and male lambs 30.77%. It is therefore concluded that saline solution (0.9%) intravenous infusion (E5) based glucose-egg yolk intended to keep goodness fertility rate and litter size after AI of cooled semen at 5ºC.

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“…Human seminal plasma contains a variety of macro and trace elements including magnesium (Mg), copper (Cu), zinc (Zn) and iron (Fe) that have essential roles in normal functioning of semen and its quality [1][2][3][4]. Mg is required as the main cofactor for kinase enzymes [5] and acts as an intracellular calcium (Ca) antagonist [6].…”

Section: Introductionmentioning

confidence: 99%