Sodium New Houttuyfonate Affects Transcriptome and Virulence Factors of Pseudomonas aeruginosa Controlled by Quorum Sensing

Zhao, Yeye; Mei, Longfei; Si, Yuanqing; Wu, Jiadi; Shao, Jing; Wang, Tianming; Ge, Yan; Wang, Changzhong; Wu, Donghui

doi:10.3389/fphar.2020.572375

Cited by 10 publications

(6 citation statements)

References 50 publications

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“…Hence, QS is a critical target for the design of novel antimicrobial therapeutics. Some TCMMs have been shown to inhibit QS in addition to their antimicrobial activity Frontiers in Pharmacology frontiersin.org (Lee et al, 2014;Chen et al, 2016;Zhao et al, 2020). In this study, the QS inhibitory activity of thirty-four TCMMs was investigated by biosensor CV026.…”

Section: Discussionmentioning

confidence: 99%

Effect of traditional Chinese medicine monomers interfering with quorum-sensing on virulence factors of extensively drug-resistant Acinetobacter baumannii

2023

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The antimicrobial resistance of Acinetobacter baumannii (A. baumannii) clinical isolates has emerged as a great threat to public health. Quorum sensing (QS) is one of the resistance mechanisms for drug-resistant A. baumannii. Interfering with QS is a promising strategy to combat infections caused by drug-resistant bacteria. This study explored the QS inhibition ability of thirty-four traditional Chinese medicine monomers (TCMMs) and assessed the effect of QS inhibitors (QSIs) on the virulence factors of twelve extensively drug-resistant A. baumannii (XDRAB) strains. Nine traditional Chinese medicine monomers, such as caffeic acid, cinnamic acid, and myricetin, were found to be able to inhibit the bacterial QS. Then, at 1/8 of the minimal inhibitory concentration, we found that these QSIs inhibited extensively drug-resistant A. baumannii adhesion and biofilm formation and downregulated the expression levels of virulence-associated genes (abaI, abaR, csuE, pgaA, and bap). In conclusion, nine traditional Chinese medicine monomers have QS inhibitory activity and may downregulate QS genes to interfere with the QS system, which could inhibit the expression of extensively drug-resistant A. baumannii virulence factors. These results suggest that traditional Chinese medicine monomers could develop as novel anti-virulence compounds to control extensively drug-resistant A. baumannii infections.

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Section: Discussionmentioning

confidence: 99%

Effect of traditional Chinese medicine monomers interfering with quorum-sensing on virulence factors of extensively drug-resistant Acinetobacter baumannii

2023

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“…Structurally, SNH is more stable than houttuynin [ 42 ]. Currently, SNH is an antibacterial agent mainly for inflammatory diseases, such as chronic bronchitis, pneumonia, and other respiratory diseases, which is effective for infections caused by Staphylococcus aureus , Streptococcus pneumoniae, Streptococcus mutans, Pseudomonas aeruginosa and Candida albicans [ 34 , 43 , 44 , 45 , 46 ]. MET, as a common clinical antibacterial agent against anaerobic bacteria, is widely used in the treatment of infectious diseases.…”

Section: Discussionmentioning

confidence: 99%

Sodium New Houttuyfonate Inhibits Cancer-Promoting Fusobacterium nucleatum (Fn) to Reduce Colorectal Cancer Progression

Jia

Zhao

et al. 2022

Cancers

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Colorectal cancer (CRC) is a major cause of morbidity and mortality worldwide. Recent studies showed that the common anaerobe Fusobacterium nucleatum (Fn) is closely associated with a higher risk for carcinogenesis, metastasis, and chemoresistance of CRC. However, there is no specific antimicrobial therapy for CRC treatment. Herbal medicine has a long history of treating diseases with remarkable effects and is attracting extensive attention. In this study, we tested six common phytochemicals for their antimicrobial activities against Fn and whether anti-Fn phytochemicals can modulate CRC development associated with Fn. Among these antimicrobials, we found that SNH showed the highest antimicrobial activity and little cytotoxicity toward cancer cells and normal cells in vitro and in vivo. Mechanistically, SNH may target membrane-associated FadA, leading to FadA oligomerization, membrane fragmentation and permeabilization. More importantly, SNH blocked the tumor-promoting activity of Fn and Fn-associated cancer-driven inflammation, thus improving the intestinal barrier damaged by Fn. SNH reduced Fn load in the CRC-cells-derived mice xenografts with Fn inoculation and significantly inhibited CRC progression. Our data suggest that SNH could be used for an antimicrobial therapy that inhibits Fn and cancer-driven inflammation of CRC. Our results provide an important foundation for future gut microbiota-targeted clinical treatment of CRC.

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“…In a previous study, sodium houttuyfonate effectively inhibited the synthetic lasl gene of AHL and reduced the level of expression, and the AHL receptor and the transcriptional regulator LasR were also inhibited, thereby downregulating the expression of the virulence factors pyocyanin and LasA; therefore, the PA population size can be effectively controlled through AHL mediation (Wu et al, 2014). Moreover, in a treatment evaluation of sodium houttuyfonate, the expression of the rhll and pqsA genes, which play key roles in the QS system, was significantly reduced and interfered with the production of pyocyanin; additionally, biofilm formation was monitored, and with the exception of lasA, the expression of the LasB, LecA, phzM, pqsA, and pilG genes was affected, which further inhibited the activation of PA virulence factors and biofilm formation (Zhao et al, 2020). These research results provide new insights for anti-PA activity.…”

Section: Anti-pseudomonas Aeruginosa Activitymentioning

confidence: 99%

Houttuynia cordata Thunb: An Ethnopharmacological Review

Zhao

Deng

et al. 2021

Front. Pharmacol.

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Houttuynia cordata Thunb (H. cordata; Saururaceae) is widely distributed in Asian regions. It plays an important role in traditional health care and disease treatment, as its aboveground stems and leaves have a long medicinal history in China and are used in the treatment of pneumonia and lung abscess. In clinical treatment, it can usually be combined with other drugs to treat dysentery, cold, fever, and mumps; additionally, H. cordata is an edible plant. This review summarizes detailed information on the phytochemistry and pharmacological effects of H. cordata. By searching the keywords “H. cordata and lung”, “H. cordata and heart”, “H. cordata and liver”, and “H. cordata and inflammation” in PubMed, Web of Science and ScienceDirect, we screened out articles with high correlation in the past ten years, sorted out the research contents, disease models and research methods of the articles, and provided a new perspective on the therapeutic effects of H. cordata. A variety of its chemical constituents are characteristic of medicinal plants, the chemical constituents were isolated from H. cordata, including volatile oils, alkaloids, flavonoids, and phenolic acids. Flavonoids and volatile oils are the main active components. In pharmacological studies, H. cordata showed organ protective activity, such as reducing the release of inflammatory factors to alleviate lung injury. Moreover, H. cordata regulates immunity, enhances the immune barriers of the vagina, oral cavity, and intestinal tract, and combined with the antibacterial and antiviral activity of its extract, effectively reduces pathogen infection. Furthermore, experiments in vivo and in vitro showed significant anti-inflammatory activity, and its chemical derivatives exert potential therapeutic activity against rheumatoid arthritis. Antitumour action is also an important pharmacological activity of H. cordata, and studies have shown that H. cordata has a notable effect on lung tumour, liver tumour, colon tumour, and breast tumour. This review categorizes the biological activities of H. cordata according to modern research papers, and provides insights into disease prevention and treatment of H. cordata.

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Sodium New Houttuyfonate Affects Transcriptome and Virulence Factors of Pseudomonas aeruginosa Controlled by Quorum Sensing

Cited by 10 publications

References 50 publications

Effect of traditional Chinese medicine monomers interfering with quorum-sensing on virulence factors of extensively drug-resistant Acinetobacter baumannii

Effect of traditional Chinese medicine monomers interfering with quorum-sensing on virulence factors of extensively drug-resistant Acinetobacter baumannii

Sodium New Houttuyfonate Inhibits Cancer-Promoting Fusobacterium nucleatum (Fn) to Reduce Colorectal Cancer Progression

Houttuynia cordata Thunb: An Ethnopharmacological Review

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