Sodium-Iodide Symporter Mediates Iodide Secretion in Rat Gastric Mucosa In Vitro

Josefsson, Martin; Evilevitch, Lena; Weström, Björn; Grunditz, Torsten; Ekblad, Eva

doi:10.1177/153537020623100306

Cited by 15 publications

(11 citation statements)

References 21 publications

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“…The latter authors reported that the distribution of SLC5A5 transcripts in the stomach epithelium was consistent with a role of SLC5A5 in the import or export of iodine, from or to the stomach contents. In the rat, iodine is actively transported into the gastric lumen and this transport is at least partly mediated by a sodium-iodide symporter ( Josefsson et al, 2006 ). In cattle the rate of iodine export by the abomasum epithelium into the abomasum is much greater than the import of iodine from the abomasum ( Miller, Swanson & Spalding, 1975 ), suggesting that the role of SLC5A5 in sheep abomasum is to export iodine into the stomach contents.…”

Section: Resultsmentioning

confidence: 99%

Epithelial, metabolic and innate immunity transcriptomic signatures differentiating the rumen from other sheep and mammalian gastrointestinal tract tissues

Xiang

Oddy

Archibald

et al. 2016

PeerJ

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Background. Ruminants are successful herbivorous mammals, in part due to their specialized forestomachs, the rumen complex, which facilitates the conversion of feed to soluble nutrients by micro-organisms. Is the rumen complex a modified stomach expressing new epithelial (cornification) and metabolic programs, or a specialised stratified epithelium that has acquired new metabolic activities, potentially similar to those of the colon? How has the presence of the rumen affected other sections of the gastrointestinal tract (GIT) of ruminants compared to non-ruminants?Methods. Transcriptome data from 11 tissues covering the sheep GIT, two stratified epithelial and two control tissues, was analysed using principal components to cluster tissues based on gene expression profile similarity. Expression profiles of genes along the sheep GIT were used to generate a network to identify genes enriched for expression in different compartments of the GIT. The data from sheep was compared to similar data sets from two non-ruminants, pigs (closely related) and humans (more distantly related).Results. The rumen transcriptome clustered with the skin and tonsil, but not the GIT transcriptomes, driven by genes from the epidermal differentiation complex, and genes encoding stratified epithelium keratins and innate immunity proteins. By analysing all of the gene expression profiles across tissues together 16 major clusters were identified. The strongest of these, and consistent with the high turnover rate of the GIT, showed a marked enrichment of cell cycle process genes (P = 1.4 E−46), across the whole GIT, relative to liver and muscle, with highest expression in the caecum followed by colon and rumen. The expression patterns of several membrane transporters (chloride, zinc, nucleosides, amino acids, fatty acids, cholesterol and bile acids) along the GIT was very similar in sheep, pig and humans. In contrast, short chain fatty acid uptake and metabolism appeared to be different between the species and different between the rumen and colon in sheep. The importance of nitrogen and iodine recycling in sheep was highlighted by the highly preferential expression of SLC14A1-urea (rumen), RHBG-ammonia (intestines) and SLC5A5-iodine (abomasum). The gene encoding a poorly characterized member of the maltase-glucoamylase family (MGAM2), predicted to play a role in the degradation of starch or glycogen, was highly expressed in the small and large intestines.Discussion. The rumen appears to be a specialised stratified cornified epithelium, probably derived from the oesophagus, which has gained some liver-like and other specialized metabolic functions, but probably not by expression of pre-existing colon metabolic programs. Changes in gene transcription downstream of the rumen also appear have occurred as a consequence of the evolution of the rumen and its effect on nutrient composition flowing down the GIT.

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Section: Resultsmentioning

confidence: 99%

Epithelial, metabolic and innate immunity transcriptomic signatures differentiating the rumen from other sheep and mammalian gastrointestinal tract tissues

Xiang

Oddy

Archibald

et al. 2016

PeerJ

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“…2), as demonstrated in the cow (Miller et al, 1975). The factors that regulate NIS expression and function in the gastrointestinal system, however, have not been identified (Josefsson et al, 2006). …”

Section: Physiology Of Iodide Metabolism and Nismentioning

confidence: 99%

The sodium iodide symporter (NIS): Regulation and approaches to targeting for cancer therapeutics

Kogai

Brent

2012

Pharmacology & Therapeutics

152

135

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“…Consistent with previous studies reporting NIS mRNA expression in rat stomach and human gastric mucosa (Kotani et al, 1998;Vayre et al, 1999), the highest NIS gene expression was observed in the stomach in deer mice. NIS in the stomach regulated (at least partially) iodide active transport into the rat gastric mucosa, and perchlorate attenuated gastric iodide transport from the serosal to the mucosal side (Josefsson et al, 2006).…”

Section: Discussionmentioning

confidence: 97%

Effects of perchlorate on sodium‐iodide symporter and pendrin gene expression in deer mice

Cheng

Liu

Gentle

et al. 2007

Environmental Toxicology

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Effects of perchlorate on sodium-iodide symporter (NIS) and pendrin gene expression in deer mice kidney and stomach were investigated. This was accomplished by isolating a partial cDNA sequence of deer mice NIS gene of 425 bps, and quantitatively analyzing NIS mRNA expression in various deer mouse tissues. The highest NIS expression level was in the stomach, followed by testes, brain, and large intestine; very low expression of NIS was observed in the lung, kidney, heart, and liver. Exposure to perchlorate through drinking water for 28 days did not significantly increase NIS gene expression in the kidney and stomach, and pendrin gene expression in the kidney. In a depuration experiment in which deer mice were exposed to perchlorate for 8-h followed by an 88-h depuration period, no significant difference was observed between the low and high exposure groups in terms of NIS or pendrin gene expression in the kidney or stomach at the end of the experiment. Furthermore, no significant linear relationship was observed between gene expression (either NIS or pendrin) in the kidney and perchlorate mass excreted via urine at day 28, average daily excretion, or total excretion mass over the 28 day exposure. Several factors could influence the effect of perchlorate exposure on NIS and pendrin gene expression in the stomach and kidney, including (1) pre-exposure to trace perchlorate through food and water perhaps resulting in adaptation (or tolerance) in these animals; (2) metabolism of perchlorate in deer mice causing only 46-61% perchlorate excreted into urine. It is also possible that there is no effect of perchlorate exposure and/or urinary excretion on NIS and pendrin gene expression, particularly in the kidney.

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Sodium-Iodide Symporter Mediates Iodide Secretion in Rat Gastric Mucosa In Vitro

Cited by 15 publications

References 21 publications

Epithelial, metabolic and innate immunity transcriptomic signatures differentiating the rumen from other sheep and mammalian gastrointestinal tract tissues

Epithelial, metabolic and innate immunity transcriptomic signatures differentiating the rumen from other sheep and mammalian gastrointestinal tract tissues

The sodium iodide symporter (NIS): Regulation and approaches to targeting for cancer therapeutics

Effects of perchlorate on sodium‐iodide symporter and pendrin gene expression in deer mice

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