Sodium Hydrosulfide Exerts a Transitional Attenuating Effect on Spermatozoa Migration &lt;I&gt;in Vitro&lt;/I&gt;

Wiliński, Bogdan; Wiliński, Jerzy; Gajda, Mariusz; Jasek, Ewa; Somogyi, E; Głowacki, Mikołaj; Sliwa, L

doi:10.3409/fb63_2.145

Cited by 7 publications

(6 citation statements)

References 29 publications

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“…As in our findings, Zhao et al found that 25 µM Na 2 S inhibits boar sperm motility although it does not affect the viability and mitochondrial membrane potential 9 . Likewise, NaHS has been shown to inhibit the motility of mouse and human spermatozoa 8,30 . In our study, the inhibitory effects of Na 2 S on sperm motility were described by using a comprehensive set of kinetic parameters provided by Computer-Assisted Sperm Analysis (CASA).…”

Section: Discussionmentioning

confidence: 99%

Divergent effect of fast- and slow-releasing H2S donors on boar spermatozoa under oxidative stress

Pintus

Jovičić

Kadlec

et al. 2020

Sci Rep

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Hydrogen sulphide (H 2 S) is involved in the physiology and pathophysiology of different cell types, but little is known about its role in sperm cells. Because of its reducing properties, we hypothesise that H 2 S protects spermatozoa against the deleterious effects of oxidative stress, a condition that is common to several male fertility disorders. This study aimed i) to determine the total antioxidant capacities of Na 2 S and GYY4137, which are fast-and slow-releasing H 2 S donors, respectively, and ii) to test whether H 2 S donors are able to protect spermatozoa against oxidative stress. We found that Na 2 S and GYY4137 show different antioxidant properties, with the total antioxidant capacity of Na 2 S being mostly unstable and even undetectable at 150 µM. Moreover, both H 2 S donors preserve sperm motility and reduce acrosome loss, although the effects were both dose and donor dependent. Within the range of concentrations tested (3-300 µM), GYY4137 showed positive effects on sperm motility, whereas Na 2 S was beneficial at the lowest concentration but detrimental at the highest. Our findings show that Na 2 S and GYY4137 have different antioxidant properties and suggest that both H 2 S donors might be used as in vitro therapeutic agents against oxidative stress in sperm cells, although the optimal therapeutic range differs between the compounds. Hydrogen sulphide (H 2 S) is the most recently discovered gaseous molecule that participates in a variety of biological functions, as do nitric oxide (NO) and carbon monoxide (CO). In mammals, H 2 S can be synthesised by enzymatic or non-enzymatic pathways 1. Overall, it seems likely that most of the H 2 S produced within an organism is generated by the H 2 S-synthesising enzymes: cystathionine γ-lyase (CSE), cystathionine β-synthase (CBS), and 3-mercaptopyruvate sulphurtransferase (3-MST), with the latter coupled with cysteine aminotransferase (CAT) 2. In the male reproductive system, the expression of H 2 S-generating enzymes has been reported in the testis 3,4 , epididymis 5 , penile corpus cavernosum 6 , and spermatozoa 7,8 , which strongly suggests that this gasotransmitter is involved in sperm physiology to some extent. In a recent study, Wang et al. found that asthenospermic men show reduced levels of H 2 S in their seminal plasma and that exogenous H 2 S supplementation improves their sperm motility 8. In contrast, in boar spermatozoa, H 2 S exerts no or negative effects on sperm motility, viability, and mitochondrial membrane potential 9. With both positive and negative effects documented, there is still controversy concerning the role of H 2 S in sperm cells. This apparent discrepancy might, at least partly, be a result of H 2 S dose-and donor-dependent effects 10. According to their chemical structure and source, H 2 S donors include inorganic salts and derivatives of phosphorodithioate, garlic extracts, thioaminoacids, and anti-inflammatory drugs 11. On the basis of their release mechanism, H 2 S donors can be classified in two categories: slow-and fast-re...

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Section: Discussionmentioning

confidence: 99%

Divergent effect of fast- and slow-releasing H2S donors on boar spermatozoa under oxidative stress

Pintus

Jovičić

Kadlec

et al. 2020

Sci Rep

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“…As a gaseous transmitter possessing myorelaxant properties, H2S is mainly generated by cystathionine-β-synthase (CBS) and CSE from L-cysteine (L-Cys). H 2 S was shown to mediate certain biological processes of the reproductive system (d'Emmanuele di Villa Bianca et al, 2009;di Villa Bianca, Cirino, & Sorrentino, 2015;Wilinski et al, 2015). Furthermore, it is shown that the L-Cys/H2S signaling is implicated in the control of human bladder activity (Fusco et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Administration of metformin alleviates atherosclerosis by promoting H2S production via regulating CSE expression

Jiang

Zuo

et al. 2019

Journal Cellular Physiology

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The therapeutic effect of metformin (Met) on atherosclerosis was studied here.Effects of methionine and Met on the induction of inflammatory response and H 2 S expression in peritoneal macrophages were evaluated. Enzyme-linked immunosorbent assay, immunohistochemistry assay, western blot, and quantitative reverse transcription polymerase chain reaction were conducted to observe the levels of cystathionine γ-lyase (CSE), DNA methyltransferases 1 (DNMT1), DNMT3a, DNMT3b, tumor necrosis factor (TNF-α), interleukin 1b (IL-1β), and hydrogen sulfide (H 2 S). Luciferase and bisulfite sequencing assays were also utilized to evaluate the CSE promoter activity as well as the methylation status of CSE in transfected cells. Methionine significantly elevated Hcy, TNF-a, H 2 S, and IL-1β expression while decreasing the level of CSE in C57BL/6 mice. In contrary, co-treatment with Methionine and Met reduced the detrimental effect of Methionine. Homocysteine (Hcy) decreased H 2 S expression while promoting the synthesis of IL-1β and TNF-α in THP-1 and raw264.7 cells. Treatment of THP-1 and raw264.7 cells with methionine and Met reduced the activity of methionine in dose dependently. Moreover, Hcy increased the expression of DNMT and elevated the level of methylation in the CSE promoter, whereas the co-treatment with methionine and Met attenuated the effects of Hcy. Methionine significantly decreased plasma level of CSE while increasing the severity of inflammatory responses and plasma level of Hcy, which in turn suppressed H 2 S synthesis and enhanced DNA hypermethylation of CSE promoter to promote the pathogenesis of atherosclerosis. In contrary, co-treatment with methionine and Met reduced the detrimental effect of methionine.

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“…The hypothesis of NO involvement in chemotaxis through affection of the ion channel function may seem intriguing, as Miraglia et al [111] observed a positive influence of NO on sperm migration. On the other hand, a recent study by Wilińsky et al [112] showed only temporal negative influence of H 2 S on sperm chemotaxis, probably due to motility inhibition. The specific mechanism and extent of involvement of the CatSper channels in the previously mentioned processes is still a matter of debate [113,114].…”

Section: No and H 2 S Interactionsmentioning

confidence: 84%

The Roles of NO and H2S in Sperm Biology: Recent Advances and New Perspectives

Kadlec

Ros-Santaella

Pintus

2020

IJMS

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After being historically considered as noxious agents, nitric oxide (NO) and hydrogen sulfide (H2S) are now listed as gasotransmitters, gaseous molecules that play a key role in a variety of cellular functions. Both NO and H2S are endogenously produced, enzymatically or non-enzymatically, and interact with each other in a range of cells and tissues. In spite of the great advances achieved in recent decades in other biological systems, knowledge about H2S function and interactions with NO in sperm biology is in its infancy. Here, we aim to provide an update on the importance of these molecules in the physiology of the male gamete. Special emphasis is given to the most recent advances in the metabolism, mechanisms of action, and effects (both physiological and pathophysiological) of these gasotransmitters. This manuscript also illustrates the physiological implications of NO and H2S observed in other cell types, which might be important for sperm function. The relevance of these gasotransmitters to several signaling pathways within sperm cells highlights their potential use for the improvement and successful application of assisted reproductive technologies.

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Sodium Hydrosulfide Exerts a Transitional Attenuating Effect on Spermatozoa Migration <I>in Vitro</I>

Cited by 7 publications

References 29 publications

Divergent effect of fast- and slow-releasing H2S donors on boar spermatozoa under oxidative stress

Divergent effect of fast- and slow-releasing H2S donors on boar spermatozoa under oxidative stress

Administration of metformin alleviates atherosclerosis by promoting H2S production via regulating CSE expression

The Roles of NO and H2S in Sperm Biology: Recent Advances and New Perspectives

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