Sodium hydrosulfide alleviates cadmium toxicity by changing cadmium chemical forms and increasing the activities of antioxidant enzymes in salix

Yang, Lanpeng; Zeng, Jing; Wang, Ping; Zhu, Jian Hua

doi:10.1016/j.envexpbot.2018.08.026

Cited by 24 publications

(7 citation statements)

References 66 publications

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“…Each treatment was replicated 3 times, and the sample was taken every 4 h. The number of cells was determined using a blood cell counting chamber. The formation of CdS by NaHS and CdCl 2 in vitro was negligible at present low Cd and H 2 S concentrations [29].…”

Section: Cellular Proliferation Analysismentioning

confidence: 64%

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Comparative transcriptome analysis uncovers roles of hydrogen sulfide for alleviating cadmium toxicity in Tetrahymena thermophila

Xu²,

et al. 2020

Preprint

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Background: Cadmium (Cd) is a nonessential heavy metal with potentially deleterious effects on different organisms. The organisms have evolved sophisticated defense system to alleviate heavy metal toxicity. Hydrogen sulfide (H2S) effectively alleviates heavy metal toxicity in plants and reduces oxidative stress in mammals. However, the function of H2S for alleviating heavy metal toxicity in aquatic organisms remains less clear. Tetrahymena thermophila is an important model organism to evaluate toxic contaminants in an aquatic environment. In this study, the molecular roles of exogenously H2S application were explored by RNA sequencing under Cd stress in T. thermophila.Results: The exposure of 30 µM Cd resulted in T. thermophila growth inhibition, cell nigrescence, and malondialdehyde (MDA) content considerably increase. However, exogenous NaHS (donor of H2S, 70 µM) significantly alleviated the Cd-induced toxicity by inhibiting Cd absorbtion, promoting CdS nanoparticles formation and improved antioxidant system. Comparative transcriptome analysis showed that the expression levels of 9152 genes changed under Cd stress (4658 upregulated and 4494 downregulated). However, only 1359 genes were differentially expressed with NaHStreatment under Cd stress (1087 upregulated and 272 downregulated). The functional categories of the differentially expressed genes (DEGs) by gene ontology (GO) revealed that the transcripts involved in the oxidation–reduction process, oxidoreductase activity, glutathione peroxidase activity, and cell redox homeostasis were the considerable enrichments between Cd stress and NaHS treatment under Cd stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that the carbon metabolism, glutathione metabolism, metabolism of xenobiotics by cytochrome P450, and ABC transporters were significantly differentially expressed components between Cd stress and NaHS treatment under Cd stress in T. thermophila. The relative expression levels of six DEGs were further confirmed through quantitative real-time polymerase chain reaction (qRT-PCR). Conclusion: NaHS alleviated Cd stress mainly through inhibiting Cd absorbtion, promoting CdS nanoparticles formation, increasing oxidation resistance, enhancing detoxification, and regulation of transport in free-living unicellular T. thermophila. These findings will expand our understanding for H2S functions in the freshwater protozoa.

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Section: Cellular Proliferation Analysismentioning

confidence: 64%

“…NaHS increased the insoluble Cd fractions in salix leaves and roots [29]. Schizosaccharomyces pombe directly scavenge the free Cd 2+ ions and the detoxi cation process occurs through the production of CdS nanoparticles [30].…”

Section: Treatment Promotes the Production Of Endogenous H 2 S Andmentioning

confidence: 99%

Comparative transcriptome analysis uncovers roles of hydrogen sulfide for alleviating cadmium toxicity in Tetrahymena thermophila

Xu²,

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…NaHS increased the insoluble Cd fractions in salix leaves and roots [ 29 ]. Schizosaccharomyces pombe directly scavenge the free Cd 2+ ions and the detoxification process occurs through the production of CdS nanoparticles [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

“…100 μM H 2 S supplementation exhibited an inhibitory effect on Cd uptake and accumulation in rice [ 13 ]. 300 μM H 2 S alleviates Cd toxicity by changing Cd chemical forms and increasing the activities of antioxidant enzymes in salix [ 29 ]. We found that 70 μM NaHS promoted the proliferation of T. thermophila and alleviated Cd toxicity by inhibiting the accumulation of Cd in cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis uncovers roles of hydrogen sulfide for alleviating cadmium toxicity in Tetrahymena thermophila

Tao

et al. 2021

BMC Genomics

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Background Cadmium (Cd) is a nonessential heavy metal with potentially deleterious effects on different organisms. The organisms have evolved sophisticated defense system to alleviate heavy metal toxicity. Hydrogen sulfide (H2S) effectively alleviates heavy metal toxicity in plants and reduces oxidative stress in mammals. However, the function of H2S for alleviating heavy metal toxicity in aquatic organisms remains less clear. Tetrahymena thermophila is an important model organism to evaluate toxic contaminants in an aquatic environment. In this study, the molecular roles of exogenously H2S application were explored by RNA sequencing under Cd stress in T. thermophila. Results The exposure of 30 μM Cd resulted in T. thermophila growth inhibition, cell nigrescence, and malondialdehyde (MDA) content considerably increase. However, exogenous NaHS (donor of H2S, 70 μM) significantly alleviated the Cd-induced toxicity by inhibiting Cd absorbtion, promoting CdS nanoparticles formation and improving antioxidant system. Comparative transcriptome analysis showed that the expression levels of 9152 genes changed under Cd stress (4658 upregulated and 4494 downregulated). However, only 1359 genes were differentially expressed with NaHS treatment under Cd stress (1087 upregulated and 272 downregulated). The functional categories of the differentially expressed genes (DEGs) by gene ontology (GO) revealed that the transcripts involved in the oxidation–reduction process, oxidoreductase activity, glutathione peroxidase activity, and cell redox homeostasis were the considerable enrichments between Cd stress and NaHS treatment under Cd stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that the carbon metabolism, glutathione metabolism, metabolism of xenobiotics by cytochrome P450, and ABC transporters were significantly differentially expressed components between Cd stress and NaHS treatment under Cd stress in T. thermophila. The relative expression levels of six DEGs were further confirmed through quantitative real-time polymerase chain reaction (qRT-PCR). Conclusion NaHS alleviated Cd stress mainly through inhibiting Cd absorbtion, promoting CdS nanoparticles formation, increasing oxidation resistance, and regulation of transport in free-living unicellular T. thermophila. These findings will expand our understanding for H2S functions in the freshwater protozoa.

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“…Next, TopGO enrichment analysis was performed to obtain a detailed classification through false discovery rate (FDR) adjusted P-value of < 0.05 as the cutoff ( [35]. We found that 70 µM H 2 S promoted the proliferation of T. thermophila and alleviated Cd toxicity by inhibiting the accumulation of Cd in cells (Fig.…”

Section: Characterization Of the Sequenced Illumina Librariesmentioning

confidence: 99%

Comparative Transcriptome Analysis Uncovers Roles of Hydrogen Sulfide for Alleviating Cadmium Toxicity in Tetrahymena Thermophila

Xu²,

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundCadmium (Cd) is a nonessential heavy metal with potentially deleterious effects on different organisms. The organisms have evolved sophisticated defense system to alleviate heavy metal toxicity. Hydrogen sulfide (H2S) effectively alleviates heavy metal toxicity in plants. However, the function of H2S for alleviating heavy metal toxicity in aquatic organisms remains less clear. Tetrahymena thermophila is an important model organism to evaluate toxic contaminants in an aquatic environment. In this study, the molecular roles of exogenously H2S application were explored by RNA sequencing under Cd stress in T. thermophila.ResultsThe exposure of 30 µM Cd resulted in T. thermophila growth inhibition, cell nigrescence, and malondialdehyde (MDA) content considerably increase. However, exogenous H2S (70 µM) significantly alleviated the Cd-induced toxicity and improved antioxidant system. Comparative transcriptome analysis showed that the expression levels of 9152 genes changed under Cd stress (4658 upregulated and 4494 downregulated). However, only 1359 genes were differentially expressed with H2S treatment under Cd stress (1087 upregulated and 272 downregulated). The functional categories of the differentially expressed genes (DEGs) by gene ontology (GO) revealed that the transcripts involved in the oxidation–reduction process, oxidoreductase activity, glutathione peroxidase activity, and cell redox homeostasis were the considerable enrichments between Cd stress and H2S treatment under Cd stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that the carbon metabolism, glutathione metabolism, metabolism of xenobiotics by cytochrome P450, and ABC transporters were significantly differentially expressed components between Cd stress and H2S treatment under Cd stress in T. thermophila. The relative expression levels of six DEGs were further confirmed through quantitative real-time polymerase chain reaction (qRT-PCR).ConclusionH2S alleviated Cd stress mainly through increasing oxidation resistance, enhancing detoxification, and regulation of transport in free-living unicellular T. thermophila. These findings will expand our understanding for H2S functions in the freshwater protozoa.

show abstract

Sodium hydrosulfide alleviates cadmium toxicity by changing cadmium chemical forms and increasing the activities of antioxidant enzymes in salix

Cited by 24 publications

References 66 publications

Comparative transcriptome analysis uncovers roles of hydrogen sulfide for alleviating cadmium toxicity in Tetrahymena thermophila

Comparative transcriptome analysis uncovers roles of hydrogen sulfide for alleviating cadmium toxicity in Tetrahymena thermophila

Comparative transcriptome analysis uncovers roles of hydrogen sulfide for alleviating cadmium toxicity in Tetrahymena thermophila

Comparative Transcriptome Analysis Uncovers Roles of Hydrogen Sulfide for Alleviating Cadmium Toxicity in Tetrahymena Thermophila

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