Sodium hyaluronate supplemented culture medium combined with joint-simulating mechanical loading improves chondrogenic differentiation of human mesenchymal stem cells

Monaco, Graziana; Haj, Alicia J. El; Alini, Mauro; Stoddart, Martin J.

doi:10.22203/ecm.v041a40

Cited by 7 publications

(4 citation statements)

References 57 publications

(50 reference statements)

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“… 20 Similarly, COL2A1 also increased due to mechanical loading, and while not statistically significant (due to non-detected gene expression in UL), the highest gene expression values were reproducibly found in samples loaded within the MWB. Moreover, as shown within our previous 4-station bioreactor, 12 , 21 mechanical stimulation increased PRG4 expression, a mechano-regulated protein that contains sGAGs and plays an important role in the lubrication of articular joints. 22 …”

Section: Discussionsupporting

confidence: 75%

A multi-well bioreactor for cartilage tissue engineering experiments

Ladner,

Kasper,

Armiento

et al. 2023

iScience

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Section: Discussionsupporting

confidence: 75%

A multi-well bioreactor for cartilage tissue engineering experiments

Ladner,

Kasper,

Armiento

et al. 2023

iScience

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“…Surprisingly little is known on the mechano-competence of MSC-derived chondrocytes. Most in vitro studies applying mechanical loading to MSC-based tissue engineering constructs investigated whether load can enhance the efficiency of chondrogenic conversion to replace essential pro-chondrogenic factors like transforming growth factor (TGF)-β [ 10 – 16 ]. Alternatively, it was attempted to reproduce the physical microenvironment in the joint to increase the mechanical stability of MSC-derived neocartilage [ 17 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

NFκB inhibition to lift the mechano-competence of mesenchymal stromal cell-derived neocartilage toward articular chondrocyte levels

et al. 2022

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Background Fully functional regeneration of skeletal defects by multipotent progenitor cells requires that differentiating cells gain the specific mechano-competence needed in the target tissue. Using cartilage neogenesis as an example, we asked whether proper phenotypic differentiation of mesenchymal stromal cells (MSC) into chondrocytes in vitro will install the adequate biological mechano-competence of native articular chondrocytes (AC). Methods The mechano-competence of human MSC- and AC-derived neocartilage was compared during differentiation for up to 35 days. The neocartilage layer was subjected to physiologic dynamic loading in a custom-designed bioreactor and assayed for mechano-sensitive gene and pathway activation, extracellular matrix (ECM) synthesis by radiolabel incorporation, nitric oxide (NO) and prostaglandin E2 (PGE2) production. Input from different pathways was tested by application of agonists or antagonists. Results MSC and AC formed neocartilage of similar proteoglycan content with a hardness close to native tissue. Mechano-stimulation on day 21 and 35 induced a similar upregulation of mechano-response genes, ERK phosphorylation, NO production and PGE2 release in both groups, indicating an overall similar transduction of external mechanical signals. However, while AC maintained or enhanced proteoglycan synthesis after loading dependent on tissue maturity, ECM synthesis was always significantly disturbed by loading in MSC-derived neocartilage. This was accompanied by significantly higher COX2 and BMP2 background expression, > 100-fold higher PGE2 production and a weaker SOX9 stimulation in response to loading in MSC-derived neocartilage. Anabolic BMP-pathway activity was not rate limiting for ECM synthesis after loading in both groups. However, NFκB activation mimicked the negative loading effects and enhanced PGE2 production while inhibition of catabolic NFκB signaling rescued the load-induced negative effects on ECM synthesis in MSC-derived neocartilage. Conclusions MSC-derived chondrocytes showed a higher vulnerability to be disturbed by loading despite proper differentiation and did not acquire an AC-like mechano-competence to cope with the mechanical stress of a physiologic loading protocol. Managing catabolic NFκB influences was one important adaptation to install a mechano-resistance closer to AC-derived neocartilage. This new knowledge asks for a more functional adaptation of MSC chondrogenesis, novel pharmacologic co-treatment strategies for MSC-based clinical cartilage repair strategies and may aid a more rational design of physical rehabilitation therapy after AC- versus MSC-based surgical cartilage intervention.

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“…). According to Altman and other scholars, intraarticular injection of sodium hyaluronate can reduce cartilage catabolism activity, and then reduce chondrocyte apoptosis and increase chondrocyte proliferation, thus inhibit the role of pro-inflammatory mediators and pain mediators[ 17 ]. This study showed that the excellent and good rate of rehabilitation in the group A was raised than group B.…”

Section: Discussionmentioning

confidence: 99%

Effect of sodium hyaluronate combined with rehabilitation training on knee joint injury caused by golf

Chen,

2024

World J Clin Cases

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BACKGROUND In high-intensity sports like golf, knee joints are prone to injury, leading to pain, limited mobility, and decreased quality of life. Traditional treatment methods typically involve rehabilitation exercises, but their effectiveness may be limited. In recent years, sodium hyaluronate has emerged as a widely used biomedical material in the treatment of joint diseases. AIM To explore the effect of sodium hyaluronate combined with rehabilitation training on pain degree, flexion range of motion and motor function of knee joint injured by golf. METHODS Eighty patients with knee joint injury caused by golf were randomly divided into control (group B) and observation group (group A). The group B was treated with rehabilitation training, and the group A was treated with sodium hyaluronate combined with rehabilitation training. The clinical efficacy, range of motion and function of knee joint, quality of life and inflammatory factors were compared. RESULTS The excellent and good rate of rehabilitation in the group A was raised than group B. At 6 weeks and 3 months after treatment, the range of motion of the two groups was raised than that before treatment, and that of the group A was raised than group B. After treatment, the scores of Lysholm and International Knee Documentation Committee (IKDC) in the group A were raised, and those in the group A were raised than group B. The VAS score of the two groups was reduced than that of the group B, and the SF-36 score of the group A was reduced than group B. The interleukin (IL)-1 β, IL-8 and tumor necrosis factor-α in the two groups were reduced, and those in the group A were reduced than group B. CONCLUSION Sodium hyaluronate combined with rehabilitation training has a good clinical effect in the treatment of patients with knee joint injury caused by golf, which relieve pain, maintain knee joint function and improve patients' life quality.

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Sodium hyaluronate supplemented culture medium combined with joint-simulating mechanical loading improves chondrogenic differentiation of human mesenchymal stem cells

Cited by 7 publications

References 57 publications

A multi-well bioreactor for cartilage tissue engineering experiments

A multi-well bioreactor for cartilage tissue engineering experiments

NFκB inhibition to lift the mechano-competence of mesenchymal stromal cell-derived neocartilage toward articular chondrocyte levels

Effect of sodium hyaluronate combined with rehabilitation training on knee joint injury caused by golf

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