Sodium hyaluronate (hyaluronic acid) promotes migration of human corneal epithelial cells in vitro

Abstract

Purpose: Sodium hyaluronate (hyaluronic acid) is known to promote corneal epithelial wound healing in vivo and in vitro, in animal experiments. Sodium hyaluronate is the ligand for CD44, a cell surface adhesion molecule which has been found on normal human corneal epithelial cells. The purpose of this study was to investigate the effect of sodium hyaluronate on human corneal epithelial cell migration, proliferation, and CD44 receptor expression. Methods: Human corneal epithelial cell cultures were established from 32 donor corneoscleral rims and maintained separately in three different culture conditions: (1) culture medium only, (2) sodium hyaluronate enriched (0.6 mg/ml) medium, and (3) hydroxypropylmethylcellulose enriched (2.5 mg/ml) medium. The total area of migrating epithelial cell sheets in each case was measured by planimetry on days 4, 8, 12, and 16. Cytospin preparations of cells cultured in the different culture conditions were examined immunohistochemically for proliferation and CD44 receptor expression using antibodies directed against Ki67 and CD44 respectively. Results: Cells cultured in the presence of sodium hyaluronate showed significantly increased migration at days 12 and 16 (Friedmen test: p = 0.0012, day 16; p = ,0.001, day 12) compared with cells cultured in the other media. There was no difference in cell proliferation (Ki67) or CD44 expression on cells cultured in the different culture conditions. Conclusions: Sodium hyaluronate promotes migration but not proliferation or CD44 expression on human corneal epithelial cells in vitro. The beneficial effect of sodium hyaluronate in corneal wound healing is likely to be related to rapid migration of cells leading to rapid wound closure. This may be facilitated by the adhesion between CD44 on the cells and hyaluronic acid, which coats the surface of the denuded cornea.

Molecular effects of sodium hyaluronate on the healing of avian supracoracoid tendon tear: According to in situ hybridization and real‐time polymerase chain reaction

“…29 HA has been shown to reduce pathologic changes in articular fluid. 29 Gomes et al 30 reported HA promoted migration but not proliferation on human corneal epithelial cell in vitro. They suggested the beneficial effect of HA in corneal wound healing was likely to be related to rapid migration of cells.…”

Aplicações oftalmológicas do ácido hialurônico

“…Supõe-se que a interação do AH com a superfície corneal provocaria o aumento do número de receptores CD44, uma glicoproteína transmembrana envolvida na re-epitelização corneal (18) . Gomes et al, compararam o crescimento de células epiteliais humanas em três meios de cultura diferentes, um deles enriquecido com NaAH 0,6%.…”

“…Gomes et al, compararam o crescimento de células epiteliais humanas em três meios de cultura diferentes, um deles enriquecido com NaAH 0,6%. Houve maior aumento da migração celular no meio enriquecido com NaAH, fazendo-os concluir que NaAH exerce maior efeito na migração das células do epitélio corneal humano quando comparado com os outros meios de cultura estudados (18) .…”

Tempo de reepitelização corneana com a instilação de colírio contendo hialuronato de sódio e carboximetilcelulose

“…Para que haja transparência da córnea é preciso que seu epitélio e filme lacrimal estejam íntegros (2,3) . O tempo de cicatri zação da córnea, na presença de lesão epitelial, pode influenciar em sua transparência e na qualidade óptica resultante (4) .…”