Sodium houttuyfonate in vitro inhibits biofilm dispersion and expression of bdlA in Pseudomonas aeruginosa

Wang, Tianming; Huang, Weifeng; Duan, Qiangjun; Wang, Jian; Cheng, Hongwei; Shao, Jing; Li, Fang; Wu, Donghui

doi:10.1007/s11033-018-4497-9

Cited by 17 publications

(11 citation statements)

References 36 publications

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“…They also reported that the combined application of sodium houttuyfonate and levofloxacin act in a better manner to inhibit biofilm formation. Sodium houttuyfonate, a plantderived anti-neuropeptide, effectively disrupts biofilm dispersion in P. aeruginosa (Wang et al, 2019). Naringin, a flavanone glycoside extracted from citrus and grapefruits, was found to be more effective against P. aeruginosa biofilms in comparison with individual treatment of marketed antibiotics ciprofloxacin and tetracycline (Dey et al, 2020).…”

Section: Combination Therapymentioning

confidence: 99%

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Mishra¹,

Panda²,

et al. 2020

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Pathogenic microorganisms and their chronic pathogenicity are significant concerns in biomedical research. Biofilm-linked persistent infections are not easy to treat due to resident multidrug-resistant microbes. Low efficiency of various treatments and in vivo toxicity of available antibiotics drive the researchers toward the discovery of many effective natural anti-biofilm agents. Natural extracts and natural product-based antibiofilm agents are more efficient than the chemically synthesized counterparts with lesser side effects. The present review primarily focuses on various natural anti-biofilm agents, i.e., phytochemicals, biosurfactants, antimicrobial peptides, and microbial enzymes along with their sources, mechanism of action via interfering in the quorum-sensing pathways, disruption of extracellular polymeric substance, adhesion mechanism, and their inhibitory concentrations existing in literature so far. This study provides a better understanding that a particular natural anti-biofilm molecule exhibits a different mode of actions and biofilm inhibitory activity against more than one pathogenic species. This information can be exploited further to improve the therapeutic strategy by a combination of more than one natural anti-biofilm compounds from diverse sources.

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Section: Combination Therapymentioning

confidence: 99%

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Mishra¹,

Panda²,

et al. 2020

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“…Biofilm dispersal is the final stage of biofilm formation, which is an active process triggered by the deterioration of local conditions within biofilms [29]. This process is critical for the propagation and self-renewal of the community, allowing bacterial cells to actively escape from mature biofilms, and these released planktonic cells can diffuse into the bulk fluid to spawn novel biofilms in new locales [30]. Therefore, dispersion is not only the last stage of biofilm development but also the beginning of another biofilm life cycle.…”

Section: The Formation Of Bacterial Biofilmmentioning

confidence: 99%

Phages against Pathogenic Bacterial Biofilms and Biofilm-Based Infections: A Review

Liu

Zhang

et al. 2022

Pharmaceutics

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Bacterial biofilms formed by pathogens are known to be hundreds of times more resistant to antimicrobial agents than planktonic cells, making it extremely difficult to cure biofilm-based infections despite the use of antibiotics, which poses a serious threat to human health. Therefore, there is an urgent need to develop promising alternative antimicrobial therapies to reduce the burden of drug-resistant bacterial infections caused by biofilms. As natural enemies of bacteria, bacteriophages (phages) have the advantages of high specificity, safety and non-toxicity, and possess great potential in the defense and removal of pathogenic bacterial biofilms, which are considered to be alternatives to treat bacterial diseases. This work mainly reviews the composition, structure and formation process of bacterial biofilms, briefly discusses the interaction between phages and biofilms, and summarizes several strategies based on phages and their derivatives against biofilms and drug-resistant bacterial infections caused by biofilms, serving the purpose of developing novel, safe and effective treatment methods against biofilm-based infections and promoting the application of phages in maintaining human health.

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“…The larvae were observed continuously for 84 h and the number of deaths was recorded to determine the survival rate of each group. In view of studies that show that the LD 50 of SNH is 119.14 mg/kg (Jiang et al, 2006) and the LD 100 of SNH is 200 mg/kg (Lou et al, 2012), our research group selected the dose range 1024-32 µg/mL for SH in previous experiments (Wang et al, 2019;Zhao et al, 2019).…”

Section: Detection Of Camp Levelsmentioning

confidence: 99%

Sodium New Houttuyfonate Inhibits Candida albicans Biofilm Formation by Inhibiting the Ras1-cAMP-Efg1 Pathway Revealed by RNA-seq

Zhao

et al. 2020

Front. Microbiol.

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Here, we aim to investigate the antifungal effect and mechanism of action of sodium new houttuyfonate (SNH) against Candida albicans . Microdilution analysis results showed that SNH possesses potent inhibitory activity against C. albicans SC5314, with a MIC 80 of 256 μg/mL. Furthermore, we found that SNH can effectively inhibit the initial adhesion of C. albicans . Inverted microscopy, crystal violet staining, scanning electron microscopy and confocal laser scanning microscopy results showed that morphological changes during the transition from yeast to hypha and the biofilm formation of C. albicans are repressed by SNH treatment. We also found that SNH can effectively inhibit the biofilm formation of clinical C. albicans strains (Z103, Z3044, Z1402, and Z1407) and SNH in combination with fluconazole, berberine chloride, caspofungin and itraconazole antifungal agents can synergistically inhibit the biofilm formation of C. albicans . Eukaryotic transcriptome sequencing and qRT-PCR results showed that SNH treatment resulted in significantly down-regulated expression in several biofilm formation related genes in the Ras1-cAMP-Efg1 pathway ( ALS1, ALA1, ALS3, EAP1, RAS1, EFG1, HWP1 , and TEC1 ) and significantly up-regulated expression in yeast form-associated genes ( YWP1 and RHD1 ). We also found that SNH can effectively reduce the production of key messenger cAMP in the Ras1-cAMP-Efg1 pathway. Furthermore, using Galleria mellonella as an in vivo model we found that SNH can effectively treat C. albican s infection in vivo . Our presented results suggest that SNH exhibits potential antibiofilm effects related to inhibiting the Ras1-cAMP-Efg1 pathway in the biofilm formation of C. albicans .

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Sodium houttuyfonate in vitro inhibits biofilm dispersion and expression of bdlA in Pseudomonas aeruginosa

Cited by 17 publications

References 36 publications

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Phages against Pathogenic Bacterial Biofilms and Biofilm-Based Infections: A Review

Sodium New Houttuyfonate Inhibits Candida albicans Biofilm Formation by Inhibiting the Ras1-cAMP-Efg1 Pathway Revealed by RNA-seq

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