Sodium fusidate (fusidin) ameliorates the course of monophasic experimental allergic encephalomyelitis in the Lewis rat

Abstract

We have evaluated the effect of the immunosuppressant sodium fusidate (fusidin) on the course of acute monophasic experimental encephalomyelitis (EAE) in male Lewis rats. Prophylactic treatment with fusidin, 80 or 120 mg/kg bd wt., markedly ameliorated the course of the disease in rats immunized with myelin basic proteins in complete Freund's adjuvant, entailing delayed onset of symptoms, lower clinical scores and more rapid recovery than PBS-treated control rats. The fusidin-treated, immunized rats exhibited milder mononuclear cell infiltration of brains and spinal cords than control animals. These data provide further evidence for the anti-inflammatory effect of fusidin and suggest that this drug may be valuable for the treatment of human multiple sclerosis.

Specific and Strain-Independent Effects of Dexamethasone in the Prevention and Treatment of Experimental Autoimmune Encephalomyelitis in Rodents

“…The disease typically progresses from mild clinical signs to severe paralysis, occasionally within a matter of hours. The rats recover spontaneously, usually by day 18–20 [21, 39, 40, 52]. In this study, initial clinical signs of EAE appeared at day 9, and the first signs of recovery occurred at day 18 (Fig.…”

“…MBP‐induced EAE in Lewis rats Lewis rats were immunized by subcutaneous injections at the base of the tail with 50 μg guinea pig MBP (Sigma‐Aldrich, Milan, Italy) and 2 mg M. tuberculosis strain H37RA (Difco) in IFA (Difco) to make a 1:1 emulsion as previously described [39, 40].…”

“…For the early therapeutic regimen, Dex was administered from day 7 post‐immunization until day 30 for mouse and DA rat EAE. Therapy was discontinued on day 18 for Lewis rat EAE taking into account the different timing of clinical signs development and remission of the disease that characterizes monophasic Lewis rat EAE [21, 39]. Control animals were treated with sterile water under the same experimental conditions.…”

N-Palmitoylethanolamine Administration Ameliorates the Clinical Manifestation and Progression of Experimental Autoimmune Encephalomyelitis in Rodents

“…The prophylactic regimen for Lewis rat EAE study consisted of daily injections of PEA (1Omg/kg) or an equal volume of vehicle (10% polyethylene glycol and 5% TWEEN 80 sterile distilled water) (12) for 40 days starting from the day of immunization until day 18, taking into account the different timing ofthe development of clinical signs and remission of the disease that characterizes monophasic Lewis rat EAE (10). The therapeutic treatment regimen for mouse EAE study consisted of daily O.lmL intraperitoneal injections of PEA (lOmg/kg) or an equal volume of vehicle (10% polyethylene glycol and 5% TWEEN 80 sterile distilled water) administered to EAE mice beginning on the second day of observed clinical signs of disease (13).…”

“…Lewis rats were immunized by subcutaneous injections at the base of the tail with 50 lg guinea pig MBP (Sigma-Aldrich, Milan, Italy) and 2 mg M. tuberculosis strain H37RA (Difco) in IFA (Difco) to make a 1:1 emulsion as previously described (10).…”