Sodium fluoride influences calcium metabolism resulting from the suppression of osteoclasts in the scales of nibbler fish Girella punctata

Sato, Masayuki; Yachiguchi, Koji; Motohashi, Ken; Yaguchi, Yukio; Tabuchi, Yoshiaki; Kitani, Yoichiro; Ikari, Takahiro; Ogiso, Satoshi; Sekiguchi, Toshio; Hải, Trần Ngọc; Hương, Đỗ Thị Thanh; Hoàng, Nguyễn Viết; Urata, Makoto; Mishima, Hiroyuki; Hattori, Atsuhiko; Suzuki, Nobuo

doi:10.1007/s12562-017-1086-0

Cited by 12 publications

(11 citation statements)

References 30 publications

(44 reference statements)

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“…In addition, we found that ALP activity appeared to have decreased remarkably. ALP activity is also known as an osteoblastic marker [11,21,30], although ALP is a marker of liver diseases [31,32]. In the present study, we indicated that the mRNA expression of the osteoblastic marker COL1A1 decreased significantly.…”

Section: Discussionsupporting

confidence: 55%

“…The separated plasma was immediately frozen and kept at -80 • C until use. The plasma total Ca levels (mg/dL) were determined using an assay kit (Calcium E, FUJIFILM Wako Pure Chemical Corporation) as described in Suzuki et al (2017) [20] and Sato et al (2017) [21]. The plasma Pi levels (mg/dL) were measured using an assay kit (IP-II, Kyowa MEDEX Co., Ltd., Tokyo, Japan).…”

Section: Effects Of Baa On Plasma Ca and Pi Levels In Nibbler Fishmentioning

confidence: 99%

“…Complementary DNA synthesis was performed using a kit (PrimeScript™ II 1st strand cDNA Synthesis Kit, Takara Bio Inc., Kusatsu, Japan). Gene-specific primers for matrix metalloproteinase-9 (MMP-9) [21] and collagen, type 1, α1 (COL1A1) [22] are indicated in Table 1. Elongation factor-1α (EF-1α) cDNA was amplified using a primer set [22] ( Table 1).…”

Section: Osteoblastic and Osteoclastic Marker Mrna Expression In Baa-mentioning

confidence: 99%

“…Elongation factor-1α (EF-1α) cDNA was amplified using a primer set [22] ( Table 1). The PCR amplification was analyzed using a real-time PCR apparatus (Mx3000p, Agilent Technologies, Santa Clara, CA, USA) [21,23]. The annealing temperature of MMP-9, COL1A1, and EF-1α was 60 • C. The MMP-9 and COL1A1 mRNA levels were normalized to the EF-1α mRNA level [24].…”

Section: Osteoblastic and Osteoclastic Marker Mrna Expression In Baa-mentioning

confidence: 99%

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Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata

Zanaty

Sawada

Kitani

et al. 2020

IJERPH

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It has been reported that spinal deformity was induced in developing fish by the addition of polycyclic aromatic hydrocarbons (PAHs). To examine the mechanism of the disruption of fish bone metabolism, the effect of benz[a]anthracene (BaA), a kind of PAH, on plasma calcium, inorganic phosphorus, osteoblasts, and osteoclasts was investigated in this study. We also measured several plasma components to analyze the toxicity of BaA on other metabolisms. BaA (1 or 10 ng/g body weight) was intraperitoneally injected (four times) into nibbler fish during breeding, for 10 days, and it was indicated, for the first time, that injecting high doses of BaA to nibbler fish induced both hypocalcemia and hypophosphatemia. Furthermore, in the scales of nibbler fish treated with high doses of BaA, both osteoclastic and osteoblastic marker messengerRNA (mRNA) expressions decreased. These results are a cause of disruption of bone metabolism and, perhaps, the induction of spinal deformities. In addition, we found that total protein, metabolic enzymes in the liver, total cholesterol, free cholesterol, and high-density lipoprotein cholesterol levels significantly decreased in BaA-injected fish. These results indicate that BaA may affect liver diseases and emphasize the importance of prevention of aquatic PAH pollution.

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Section: Discussionsupporting

confidence: 55%

Section: Effects Of Baa On Plasma Ca and Pi Levels In Nibbler Fishmentioning

confidence: 99%

Section: Osteoblastic and Osteoclastic Marker Mrna Expression In Baa-mentioning

confidence: 99%

Section: Osteoblastic and Osteoclastic Marker Mrna Expression In Baa-mentioning

confidence: 99%

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Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata

Zanaty

Sawada

Kitani

et al. 2020

IJERPH

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“…A TaqMan probe was used for Calcitonin (Figure ). Elongation factor 1 alpha ( Ef1α ) was used for the standardization procedure …”

Section: Methodsmentioning

confidence: 99%

Melatonin is a potential drug for the prevention of bone loss during space flight

Ikegame

Hattori

Tabata

et al. 2019

Journal of Pineal Research

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Astronauts experience osteoporosis‐like loss of bone mass because of microgravity conditions during space flight. To prevent bone loss, they need a riskless and antiresorptive drug. Melatonin is reported to suppress osteoclast function. However, no studies have examined the effects of melatonin on bone metabolism under microgravity conditions. We used goldfish scales as a bone model of coexisting osteoclasts and osteoblasts and demonstrated that mRNA expression level of acetylserotonin O‐methyltransferase, an enzyme essential for melatonin synthesis, decreased significantly under microgravity. During space flight, microgravity stimulated osteoclastic activity and significantly increased gene expression for osteoclast differentiation and activation. Melatonin treatment significantly stimulated Calcitonin (an osteoclast‐inhibiting hormone) mRNA expression and decreased the mRNA expression of receptor activator of nuclear factor κB ligand (a promoter of osteoclastogenesis), which coincided with suppressed gene expression levels for osteoclast functions. This is the first study to report the inhibitory effect of melatonin on osteoclastic activation by microgravity. We also observed a novel action pathway of melatonin on osteoclasts via an increase in CALCITONIN secretion. Melatonin could be the source of a potential novel drug to prevent bone loss during space flight.

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α-Melanocyte-stimulating hormone directly increases the plasma calcitonin level and involves calcium metabolism in goldfish

et al. 2018

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The effects of a-melanocyte-stimulating hormone (a-MSH) on calcium metabolism were examined with goldfish. The scales on the left side of goldfish bodies were removed to allow the regeneration of scales under anesthesia. Thereafter, the influences of a-MSH injection (low dose: 0.1 lg/g body weight; high dose: 1 lg/g body weight) on plasma calcitonin (calcium-regulating hormone) and the calcium content of the scales were investigated. Ten days after removing the scales, we measured the plasma calcitonin and calcium content of both regenerating scales on the left side and ontogenic scales on the right side. At both doses of a-MSH injection, plasma calcitonin concentrations in the a-MSH-treated group were significantly higher than those in the control group. The mRNA expressions of a-MSH-receptors were detected in the ultimobranchial glands (secretory organ of calcitonin), indicating that a-MSH directly functions in ultimobranchial glands and promotes calcitonin secretion. Furthermore, we found that the calcium content of regenerating scales in a-MSHtreated goldfish was higher than that in control goldfish, while the calcium content of ontogenic scales on the right side was significantly decreased by a-MSH injection. There was a significant co-relationship between plasma calcitonin and the calcium content of regenerating scales. The mRNA expression of calcitonin receptors in regenerating scales was remarkably higher than that in ontogenic scales. These results imply that calcitonin functions to promote scale regeneration resulting from the inhibition of bone resorption because

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Sodium fluoride influences calcium metabolism resulting from the suppression of osteoclasts in the scales of nibbler fish Girella punctata

Cited by 12 publications

References 30 publications

Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata

Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata

Melatonin is a potential drug for the prevention of bone loss during space flight

α-Melanocyte-stimulating hormone directly increases the plasma calcitonin level and involves calcium metabolism in goldfish

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