Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation

Abstract

Excessive long-term fluoride intake is associated with several health problems, including infertility. However, limited information is available on the toxic effects of fluoride exposure on the female reproductive system, especially oocyte maturation. In this study, we investigated the toxic effect of sodium fluoride (NaF) exposure on porcine oocyte maturation and its possible underlying mechanisms. Our results showed that NaF exposure during porcine oocyte maturation inhibited cumulus cell expansion and impaired polar body extrusion. Cell cycle analysis showed that NaF exposure blocked meiotic resumption, disturbed spindle dynamics, disrupted chromosome separation, and increased aneuploidy in porcine oocytes. Moreover, NaF exposure disturbed mitochondrial function, triggered DNA damage response, and induced early apoptosis in porcine oocytes. NaF exposure also induced oxidative stress, decreased GSH level, and increased cathepsin B activity in and impaired the further development potential of porcine oocytes, as indicated by a decrease in blastocyst formation rate, increase in apoptosis, and inhibition of cell proliferation. Together, these results indicate that NaF exposure impairs the maturation capacity of porcine oocytes by inhibiting cumulus cell expansion, disturbing cytoskeletal dynamics, and blocking nuclear and cytoplasmic maturation, thus decreasing the quality and affecting the subsequent embryonic development potential of porcine oocytes.

Allicin protects porcine oocytes against damage during aging in vitro

“…Moreover, these factors are important determinants of the efficiency of embryo production in vitro. Indeed, these parameters decrease when oocytes are exposed to stresses such as aging (Lee et al, ), oxidative stress induced by H 2 O 2 or sodium fluoride (Liang et al, ; Yazaki, Hiradate, Hoshino, Tanemura & Sato, ), and heat stress induced by high temperature (Gharibzadeh et al, ). In this study, oocyte survival and PB extrusion rates were lower in the 0 AL group than in the control group, and these decreases were prevented in the 1 AL group (Table ).…”

“…Oocyte quality is a key factor determining embryo competency, regardless of whether maturation occurs under normal or stress conditions. Oocytes whose quality is decreased by aging or exposure to oxidative stress have a reduced developmental potential (Lee et al, ; Liang et al, ). Consistent with this, we found that the blastocyst formation rate was significantly lower in the 0 AL group than in the 1 AL group.…”

Antioxidant hesperetin improves the quality of porcine oocytes during aging in vitro

“…Many factors increase the developmental rate of oocytes (R. Li, Liu, Pedersen, & Callesen, ; Park, So, & Hyun, ; Shin et al, ). Oocytes damaged by aging or oxidative stress reportedly have a reduced developmental potential (Chaube et al, ; Goud et al, ; Liang et al, ). To investigate blastocyst quality, we checked the numbers of total and apoptotic cells per blastocyst.…”

“…Many factors increase the developmental rate of oocytes (R. Li, Liu, Pedersen, & Callesen, 2015;Park, So, & Hyun, 2017;Shin et al, 2018). Oocytes damaged by aging or oxidative stress reportedly have a reduced developmental potential (Chaube et al, 2005;Goud et al, 2008;Liang et al, 2017).…”

Isobutylparaben Negatively Affects Porcine Oocyte Maturation Through Increasing Oxidative Stress and Cytoskeletal Abnormalities

“…Oxidative stress is part of the detrimental factors affecting oocyte developmental competence. Excessive oxidative stress will cause a series of damage to cells and leads to apoptosis (Liang et al ). Histone modification is also necessary for oocyte maturation, and aberrant histone modification could lead to the structural abnormalities between histone and DNA, which in turn affect gene expression and induce potential damage to oocyte (Huang et al ).…”