Sodium dithionite-enhanced quality of radix scutellariae through modification of secondary metabolism

Guo, Hao; Xiaoying, Fu; Du, Hongwei; Wei, Cong; Meng, Xiangying

doi:10.4103/2230-973x.195932

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…The contents of baicalin, wogonoside, and chrysin are all high in the natural state, and furthermore, these reactions are fulfilled by only one step; therefore, the reaction was swiftly performed. Second, ROS can increase the activity of phenylalanine ammonialyase (PAL) [20,32], continuously replenishing flavonoids.…”

Section: Plos Onementioning

confidence: 99%

“…1 μs. Sodium hydrosulfite (Na 2 S 2 O 4 ) can produce O˙ - 2 spontaneously under alkaline conditions [ 19 ] and is regarded as an important carrier [ 20 ]; Na 2 S 2 O 4 can easily control O˙ - 2 production, creating stress physiology. Here, Na 2 S 2 O 4 was employed to induce stress, and we investigated the different secondary metabolites, the biological significance of flavonoids in S .…”

Section: Introductionmentioning

confidence: 99%

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Complexity of active medicinal ingredients in radix scutellariae with sodium hydrosulfite exposure

et al. 2020

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Both plants and animals are living things made up of similar cells as well as organelles, and their essence of life is the same. However, plants face more environmental stress than animals and generate excessive reactive oxygen species (ROS), a group of small molecules that can harm proteins, necessitating distinctive metabolic processes. Secondary metabolites in plants are a group of chemical components that can eliminate ROS and can also exhibit medicinal properties; therefore, herbal medicines are often closely linked to the ecological significance of secondary metabolites. Why plants contain so many, not few, active medicinal ingredients is unknown. The root of Scutellaria baicalensis, a popular herbal medicine, is rich in various flavonoids with diverse structural features. Sodium hydrosulfite (Na 2 S 2 O 4) can produce O_-2 radicals and induce physical conditions under environmental stress. Using UHPLC-ESI-Q-TOF-MS/MS analysis, a total of 25 different compounds were identified in the roots of S. baicalensis between the Na 2 S 2 O 4 groups and suitable conditions. Based on the results of the t-test (P<0.05) performed for the groups and ions with values of VIP � 2, the most significantly different chemical markers with Na 2 S 2 O 4 treatment were shi

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Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Complexity of active medicinal ingredients in radix scutellariae with sodium hydrosulfite exposure

et al. 2020

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“…Numerous studies have demonstrated that the high amount of ROS under adversity can elevate the contents of plant secondary metabolites (9), for example, the contents of active ingredients of Scutellaria baicalensis have an increase of more than 50% due to Na2S2O4 (a carrier substance for O2 •-), and the quality of Fructus schisandrae chinensis was significantly improved under the H2O2, etc. (10,11).…”

Section: Introductionmentioning

confidence: 99%

Sodium Nitroprusside Improved the Quality of Radix Saposhnikoviae through Constructed Physiological State under Adversity

Song,

Yao,

Zhao

et al. 2023

Preprint

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The quality of botanicals is closely related to the growing environment, usually with a better quality under adverse conditions. The quality of the cultivated under good conditions were heavily declined. Radix Saposhnikoviae is one of the most commonly botanicals in Asian countries, the price of the cultivated is only 1/10 to 1/3 of that of the wild, therefore improved the quality of the cultivated Radix Saposhnikoviae is an urgent issue. The fresh Radix saposhnikoviae roots were treated with 0.03, 0.1, 0.5, and 1.0 mmol/L SNP, and the activities of antioxidant enzymes, secondary metabolite synthesis enzymes such as PAL, ACC and CHS, and the contents of NO, O2·ˉ, H2O2, MDA, and secondary metabolite chromones were determined. The quality of Radix Saposhnikoviae was evaluated with antipyretic, analgesic, and anti-inflammatory effects, and inflammatory factors. The NO contents in the fresh roots of Radix saposhnikoviae were significantly increased under SNP, which led to a significant increase of O2·ˉ, H2O2, and MDA. The activities of antioxidant enzymes SOD, CAT, and POD showed an increase, reach a peak on the 2nd and the 3rd day. The activities of PAL, ACC, and CHS were also significantly enhanced, resulting in the increased secondary metabolite contents of Radix Saposhnikoviae in all groups, especially the 0.5 mmol/ L SNP, the four active ingredients, prim-O-glucosylcimifugin, cimifugin, 4'-O-β-D-glucosyl-5-O-methylvisamminol, and sec-O-glucosylhamaudol, increased by 90.5%, 252.1%, 52.2% and 153.7%, respectively, on the 3rd day, which was accomplished by biosynthesis. The pharmaceutical effects of Radix saposhnikoviae under 0.5mmol/L SNP were significantly enhanced. Exogenous SNP can induce physiological states under the adverse conditions and significantly improve the quality of Radix Saposhnikoviae, which can provide a new way for high-quality production.

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“…Similarly, the quality of Fructus schisandrae chinensis was significantly improved when treated with H 2 O 2 , etc. 13 , 14 .…”

Section: Introductionmentioning

confidence: 99%

Sodium nitroprusside improved the quality of Radix Saposhnikoviae through constructed physiological response under ecological stress

Song,

Yao,

et al. 2023

Sci Rep

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The ecological significance of secondary metabolites is to improve the adaptive ability of plants. Secondary metabolites, usually medicinal ingredients, are triggered by unsuitable environment, thus the quality of medicinal materials under adversity being better. The quality of the cultivated was heavily declined due to its good conditions. Radix Saposhnikoviae, the dried root of Saposhnikovia divaricata (Turcz.) Schischk., is one of the most common botanicals in Asian countries, now basically comes from cultivation, resulting in the market price being only 1/10 to 1/3 of its wild counterpart, so improving the quality of cultivated Radix Saposhnikoviae is of urgency. Nitric oxide (NO) plays a crucial role in generating reactive oxygen species and modifying the secondary metabolism of plants. This study aims to enhance the quality of cultivated Radix Saposhnikoviae by supplementing exogenous NO. To achieve this, sodium nitroprusside (SNP) was utilized as an NO provider and applied to fresh roots of S. divaricata at concentrations of 0.03, 0.1, 0.5, and 1.0 mmol/L. This study measured parameters including the activities of antioxidant enzymes, secondary metabolite synthesis enzymes such as phenylalanine ammonia-lyase (PAL), 1-aminocyclopropane-1-carboxylic acid (ACC), and chalcone synthase (CHS), as well as the contents of NO, superoxide radicals (O2·−), hydrogen peroxide (H2O2), malondialdehyde (MDA), and four secondary metabolites. The quality of Radix Saposhnikoviae was evaluated with antipyretic, analgesic, anti-inflammatory effects, and inflammatory factors. As a result, the NO contents in the fresh roots were significantly increased under SNP, which led to a significant increase of O2·−, H2O2, and MDA. The activities of important antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), were found to increase as well, with their peak levels observed on the 2nd and 3rd days. PAL, ACC, and CHS activities were also significantly enhanced, resulting in the increased secondary metabolite contents of Radix saposhnikoviae in all groups, especially the 0.5 mmol/L SNP. The four active ingredients, prim-O-glucosylcimifugin, cimifugin, 4′-O-β-d-glucosyl-5-O-methylvisamminol, and sec-O-glucosylhamaudol, increased by 88.3%,325.0%, 55.4%, and 283.8%, respectively, on the 3rd day. The pharmaceutical effects of Radix Saposhnikoviae under 0.5 mmol/L SNP were significantly enhanced. Exogenous SNP can induce the physiological response of S. divaricata under adverse conditions and significantly improve the quality of Radix Saposhnikoviae.

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Sodium dithionite-enhanced quality of radix scutellariae through modification of secondary metabolism

Cited by 5 publications

References 15 publications

Complexity of active medicinal ingredients in radix scutellariae with sodium hydrosulfite exposure

Complexity of active medicinal ingredients in radix scutellariae with sodium hydrosulfite exposure

Sodium Nitroprusside Improved the Quality of Radix Saposhnikoviae through Constructed Physiological State under Adversity

Sodium nitroprusside improved the quality of Radix Saposhnikoviae through constructed physiological response under ecological stress

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