Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis

Rivoira, M.A.; Marchionatti, Ana Maria Estivalete; Centeno, Viviana; Barboza, Gabriela E. Díaz de; López, M.E. Peralta; Talamoni, Nori G. Tolosa de

doi:10.1016/j.cbpa.2012.04.016

Cited by 19 publications

(15 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NaDOC also produced GSH depletion, as well as ROS generation and mitochondrial swelling, which in turn led to mitochondria mediated apoptosis. Briefly, a single high concentration of NaDOC inhibits intestinal Ca 2+ absorption via downregulation of proteins involved in the transcellular pathway, as a result of oxidative stress and apoptosis[98]. Similarly, in a rat model of type 1 Diabetes mellitus induced by streptozotocin, we have also demonstrated oxidative stress in the intestine at early stages of developing of disease, leading to inhibition of the intestinal Ca 2 + absorption.…”

Section: Actions Of Pro-oxidants On Intestinal Calcium Absorptionmentioning

confidence: 80%

Oxidative stress, antioxidants and intestinal calcium absorption

Barboza¹,

Guizzardi²,

Moine³

et al. 2017

WJG

104

View full text Add to dashboard Cite

The disequilibrium between the production of reactive oxygen (ROS) and nitrogen (RNS) species and their elimination by protective mechanisms leads to oxidative stress. Mitochondria are the main source of ROS as by-products of electron transport chain. Most of the time the intestine responds adequately against the oxidative stress, but with aging or under conditions that exacerbate the ROS and/or RNS production, the defenses are not enough and contribute to developing intestinal pathologies. The endogenous antioxidant defense system in gut includes glutathione (GSH) and GSH-dependent enzymes as major components. When the ROS and/or RNS production is exacerbated, oxidative stress occurs and the intestinal Ca2+ absorption is inhibited. GSH depleting drugs such as DL-buthionine-S,R-sulfoximine, menadione and sodium deoxycholate inhibit the Ca2+ transport from lumen to blood by alteration in the protein expression and/or activity of molecules involved in the Ca2+ transcellular and paracellular pathways through mechanisms of oxidative stress, apoptosis and/or autophagy. Quercetin, melatonin, lithocholic and ursodeoxycholic acids block the effect of those drugs in experimental animals by their antioxidant, anti-apoptotic and/or anti-autophagic properties. Therefore, they may become drugs of choice for treatment of deteriorated intestinal Ca2+ absorption under oxidant conditions such as aging, diabetes, gut inflammation and other intestinal disorders.

show abstract

Section: Actions Of Pro-oxidants On Intestinal Calcium Absorptionmentioning

confidence: 80%

Oxidative stress, antioxidants and intestinal calcium absorption

Barboza¹,

Guizzardi²,

Moine³

et al. 2017

WJG

104

View full text Add to dashboard Cite

show abstract

“…Calcium is the predominant mineral related to external and internal egg quality. Intestinal absorption of dietary calcium has a central role in the regulation of calcium homeostasis [ 7 ]. Ionized calcium is absorbed mostly in the small intestine, accounting for approximately 90% of overall calcium absorption [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary supplementation of arginine-silicate-inositol complex on absorption and metabolism of calcium of laying hens

et al. 2018

View full text Add to dashboard Cite

The effects of supplementation of arginine-silicate-inositol complex (ASI; 49.5–8.2–25 g/kg, respectively) to laying hens were investigated with respect to eggshell quality, calcium (Ca) balance, and expression of duodenal proteins related to Ca metabolism (calbindin and tight junction proteins). A total of 360 laying hens, 25 weeks old, were divided into 3 groups consisting of 6 replicate of cages, 20 birds per cage. The groups were fed a basal diet and the basal diet supplemented with 500 or 1000 mg ASI complex per kilogram for 90 days. Data were analyzed by ANCOVA using data during the first week of the adaptation period as covariates. As the ASI complex supplementation level increased, there were increases in feed intake (P < 0.0001), egg production (P < 0.001), egg weight (P < 0.0001) and eggshell weight (P < 0.001) weight, and shell thickness (P < 0.001) and decreases in feed conversion ratio and cracked egg percentage (P < 0.0001 for both). Concentrations of serum osteocalcin (P < 0.0001), vitamin D (P < 0.0001), calcium (P < 0.001), phosphorus (P < 0.001), and alkaline phosphatase (P < 0.008) as well as amounts of calcium retention (P < 0.0001) and eggshell calcium deposition (P < 0.001), and Ca balance (P < 0.0001) increased, whereas amount of calcium excretion (P < 0.001) decreased linearly in a dose-dependent manner. The ASI complex supplementation increased expressions of calcium transporters (calbindin-D28k, N sodium-calcium exchanger, plasma membrane calcium ATPase, and vitamin D receptor) and tight junction proteins (zonula occludens-1 and occludin) in the duodenum in a linear fashion (P < 0.0001 for all). In conclusion, provision of dietary ASI complex to laying hens during the peak laying period improved eggshell quality through improving calcium utilization as reflected by upregulation of genes related to the calcium metabolism. Further studies are needed to elucidate the contribution of each of the ASI complex ingredients.

show abstract

“…In fact, we have previously demonstrated that the intrinsic pathway of apoptosis is triggered by NaDOC (Rivoira et al 2012). Our data showing the enhancement in the protein expression of FAS, FASL, and in caspase-3 activity indicate that the extrinsic pathway is also involved in the apoptosis caused by NaDOC.…”

Section: Figmentioning

confidence: 48%

“…Our studies have shown that the sodium deoxycholate (NaDOC) and the ursodeoxycholic acid (UDCA) have differential effects on intestinal Ca 2+ absorption. NaDOC inhibits Ca 2+ transport across the intestine by triggering oxidative stress and apoptosis (Rivoira et al 2012). In contrast, UDCA increases intestinal Ca 2+ absorption, which seems to be mediated by VDR, leading to enhancement of protein and gene expressions of molecules involved in the Ca 2+ transcellular pathway.…”

Section: Introductionmentioning

confidence: 98%

Lithocholic acid: a new emergent protector of intestinal calcium absorption under oxidant conditions

Marchionatti

Pérez

Rivoira

et al. 2017

Biochem. Cell Biol.

Self Cite

View full text Add to dashboard Cite

LCA and 1,25(OH)D are vitamin D receptor ligands with different binding affinity. The secosteroid stimulates intestinal Ca absorption. Whether LCA alters this process remains unknown. The aim of our work was to determine the effect of LCA on intestinal Ca absorption in the absence or presence of NaDOC, bile acid that inhibits the cation transport. The data show that LCA by itself did not alter intestinal Ca absorption, but prevented the inhibitory effect of NaDOC. The concomitant administration of LCA avoided the reduction of intestinal alkaline phosphatase activity caused by NaDOC. In addition, LCA blocked a decrease caused by NaDOC on gene and protein expression of molecules involved in the transcellular pathway of intestinal Ca absorption. The oxidative stress and apoptosis triggered by NaDOC were abrogated by LCA co-treatment. In conclusion, LCA placed in the intestinal lumen protects intestinal Ca absorption against the inhibitory effects caused by NaDOC. LCA avoids the reduction of the transcellular Ca movement, apparently by blocking the oxidative stress and apoptosis triggered by NaDOC, normalizing the gene and protein expression of molecules involved in Ca movement. Therefore, LCA might become a possible treatment to improve intestinal calcium absorption under oxidant conditions.

show abstract

Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis

Cited by 19 publications

References 36 publications

Oxidative stress, antioxidants and intestinal calcium absorption

Oxidative stress, antioxidants and intestinal calcium absorption

Effects of dietary supplementation of arginine-silicate-inositol complex on absorption and metabolism of calcium of laying hens

Lithocholic acid: a new emergent protector of intestinal calcium absorption under oxidant conditions

Contact Info

Product

Resources

About