Sodium chloride improves pullulan production by Aureobasidium pullulans but reduces the molecular weight of pullulan

Wang, Dahui; Ni, Teng-Fei; Ju, Xin; Wei, Gongyuan

doi:10.1007/s00253-018-9292-y

Cited by 19 publications

(5 citation statements)

References 37 publications

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“…The pullulan production of strain ZH27 was also sensitive to changes in NaCl concentrations, with the highest pullulan titer (67.8 ± 2.5 g/L) observed at 0.4% NaCl and an increase of 11% compared with the control at 0.1% NaCl ( Figure 2 e). This change is similar to a previously reported increase of 26.7% at 0.3% NaCl [ 33 ]. Interestingly, the highest biomass of strain ZH27 occurred at 0.5% NaCl, likely due to the formation of small molecules (e.g., glycerol) for self-protection against high salinity and osmotic stress.…”

Section: Discussionsupporting

confidence: 92%

“…Consequently, enhancing carbon flow toward biomass replaced pullulan synthesis [ 34 ]. Moreover, high expressions of some enzymes (e.g., amylases) induced by high NaCl concentrations probably contributed to the reduction in pullulan titers [ 33 ]. These findings highlight the complicated interplay of factors influencing pullulan production in strain ZH27.…”

Section: Discussionmentioning

confidence: 99%

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Hyper-Production of Pullulan by a Novel Fungus of Aureobasidium melanogenum ZH27 through Batch Fermentation

Wang,

Lin,

Zhou

et al. 2023

IJMS

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Pullulan, which is a microbial exopolysaccharide, has found widespread applications in foods, biomedicines, and cosmetics. Despite its versatility, most wild-type strains tend to yield low levels of pullulan production, and their mutants present genetic instability, achieving a limited increase in pullulan production. Therefore, mining new wild strains with robust pullulan-producing abilities remains an urgent concern. In this study, we found a novel strain, namely, Aureobasidium melanogenum ZH27, that had a remarkable pullulan-producing capacity and optimized its cultivation conditions using the one-factor-at-a-time method. To elucidate the reasons that drove the hyper-production of pullulan, we scrutinized changes in cell morphology and gene expressions. The results reveal that strain ZH27 achieved 115.4 ± 1.82 g/L pullulan with a productivity of 0.87 g/L/h during batch fermentation within 132 h under the optimized condition (OC). This pullulan titer increased by 105% compared with the initial condition (IC). Intriguingly, under the OC, swollen cells featuring 1–2 large vacuoles predominated during a rapid pullulan accumulation, while these swollen cells with one large vacuole and several smaller ones were prevalent under the IC. Moreover, the expressions of genes associated with pullulan accumulation and by-product synthesis were almost all upregulated. These findings suggest that swollen cells and large vacuoles may play pivotal roles in the high level of pullulan production, and the accumulation of by-products also potentially contributes to pullulan synthesis. This study provides a novel and promising candidate for industrial pullulan production.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Hyper-Production of Pullulan by a Novel Fungus of Aureobasidium melanogenum ZH27 through Batch Fermentation

Wang,

Lin,

Zhou

et al. 2023

IJMS

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“…The rates of both specific glucose consumption and, consequently, the specific pullulan production rate improved due to the presence of NaCl, causing greater pullulan yield. Moreover, NaCl stimulates glucosyltransferase and α-phosphoglucose mutase (involved in pullulan biosynthesis) and also stimulates α-amylase (the degraded pullulan), upregulates the transcriptional levels of fks , pgm1 , and amy2 genes, enhances the driving force for ATP supply, and helps to maintain intracellular uridine diphosphate-glucose at a high level in the fungal cells [ 44 , 45 ]. However, NaCl is essential for fungal growth, as it helps regulate the osmotic pressure of the growth medium.…”

Section: Resultsmentioning

confidence: 99%

Exclusive Biosynthesis of Pullulan Using Taguchi’s Approach and Decision Tree Learning Algorithm by a Novel Endophytic Aureobasidium pullulans Strain

2023

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Pullulan is a biodegradable, renewable, and environmentally friendly hydrogel biopolymer, with potential uses in food, medicine, and cosmetics. New endophytic Aureobasidium pullulans (accession number; OP924554) was used for the biosynthesis of pullulan. Innovatively, the fermentation process was optimized using both Taguchi’s approach and the decision tree learning algorithm for the determination of important variables for pullulan biosynthesis. The relative importance of the seven tested variables that were obtained by Taguchi and the decision tree model was accurate and followed each other’s, confirming the accuracy of the experimental design. The decision tree model was more economical by reducing the quantity of medium sucrose content by 33% without a negative reduction in the biosynthesis of pullulan. The optimum nutritional conditions (g/L) were sucrose (60 or 40), K2HPO4 (6.0), NaCl (1.5), MgSO4 (0.3), and yeast extract (1.0) at pH 5.5, and short incubation time (48 h), yielding 7.23% pullulan. The spectroscopic characterization (FT-IR and 1H-NMR spectroscopy) confirmed the structure of the obtained pullulan. This is the first report on using Taguchi and the decision tree for pullulan production by a new endophyte. Further research is encouraged for additional studies on using artificial intelligence to maximize fermentation conditions.

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“…Aureobasidium pullulans [60], A. melanogenum [61], E. album [62], Aspergillus spp., Cladosporium spp. [63], P. crustosum [64], Wallemia spp.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the xerophilic potential of moulds on selected egg tempera paints on glass and wooden supports using fluorescent microscopy

Kosel

Kavčič

Legan

et al. 2021

Journal of Cultural Heritage

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Highlights• 11 fungal isolates from cultural heritage institutions' interiors were screened for xerophilic trait • Specially designed incubators were constructed to hold specific RH levels of 55 %, 63 % and 74 %• Fungal growth was monitored at low relative humidity on wood and glass supports painted by traditional artists' paints • Effects of pigments, support materials and strain variability are discussed

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Sodium chloride improves pullulan production by Aureobasidium pullulans but reduces the molecular weight of pullulan

Cited by 19 publications

References 37 publications

Hyper-Production of Pullulan by a Novel Fungus of Aureobasidium melanogenum ZH27 through Batch Fermentation

Hyper-Production of Pullulan by a Novel Fungus of Aureobasidium melanogenum ZH27 through Batch Fermentation

Exclusive Biosynthesis of Pullulan Using Taguchi’s Approach and Decision Tree Learning Algorithm by a Novel Endophytic Aureobasidium pullulans Strain

Evaluating the xerophilic potential of moulds on selected egg tempera paints on glass and wooden supports using fluorescent microscopy

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