Sodium chloride augments the antibacterial activity of a novel penaeid shrimp-derived peptide (GPCR10) against halotolerant Staphylococcus aureus

Yang, Shen; Wang, Mingyao; Gao, Jialong; Liu, Jiaxin; Jin, Ritian; Lin, Rong; Weng, Wuyin; Aweya, Jude Juventus

doi:10.1016/j.lwt.2023.115096

Cited by 3 publications

(2 citation statements)

References 63 publications

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“…Actually, there were several researches reported that the antimicrobial activities of some peptides were significantly improved when combined with NaCl. − For instance, NaCl has been supported to enhance the antimicrobial activity of nisin Z by mediating hydrophobic attraction and interactions between amino acids in nisin Z and phospholipid fatty acids in the bacterial cell membrane, leading to a bacterial cell destruction. , Similarly, NaCl also augments the antibacterial activity of GPCR10 (a penaeid shrimp-derived peptide) against halotolerant Staphylococcus aureus through enhancing the permeation of the peptide resulting in destruction of the bacteria, extrusion of cellular content, and binding to bacterial DNA . To our knowledge, this phenomenon has not been reported for peptaibiotics.…”

Section: Resultsmentioning

confidence: 99%

New Antibacterial Peptaibiotics against Plant and Fish Pathogens from the Deep-Sea-Derived Fungus Simplicillium obclavatum EIODSF 020

Liang,

Yang,

Huang

et al. 2024

J. Agric. Food Chem.

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Bacterial diseases could severely harm agricultural production. To develop new antibacterial agents, the secondary metabolites of a deep-sea-derived fungus Simplicillium obclavatum EIODSF 020 with antibacterial activities against plant and fish pathogens were investigated by a bioassay-guided approach, which led to the isolation of 11 new peptaibiotics, simplicpeptaibs A−K (1−11). They contain 16−19 residues, including β-alanine, tyrosine, or tyrosine O-sulfate, that were rarely present in peptaibiotics. Their structures were elucidated by spectroscopic analyses (NMR, HRMS, HRMS 2 , and ECD) and Marfey's method. The primary and secondary structures of novel sulfated peptaibiotic 9 were reconfirmed by single-crystal X-ray diffraction analysis. Genome sequencing of S. obclavatum EIODSF 020 allowed the detection of a gene cluster encoding two individual NRPSs (totally containing 19 modules) that was closely related to simplicpeptaib biosynthesis. Antibacterial investigations of 1−11 together with the previously isolated linear and cyclic peptides from this strain suggested the antibacterial property of this fungus was attributed to the peptaibiotics and cyclic lipopeptides. Among them, compounds 4, 6, 7, and 9 showed significant activity against the tobacco pathogen Ralstonia solanacearum or tilapia pathogens Streptococcus iniae and Streptococcus agalactiae. The antibacterial activity of 6 against R. solanacearum could be enhanced by the addition of 1% NaCl. The structure−bioactivity relationship of simplicpeptaibs was discussed.

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Section: Resultsmentioning

confidence: 99%

New Antibacterial Peptaibiotics against Plant and Fish Pathogens from the Deep-Sea-Derived Fungus Simplicillium obclavatum EIODSF 020

Liang,

Yang,

Huang

et al. 2024

J. Agric. Food Chem.

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“…S. aureus is halotolerant and thus able to grow at high concentrations of sodium chloride, however exposure to subinhibitory concentrations of sodium chloride has been reported to alter the activity of membrane active compounds, including AMPs [44][45][46] . We sought to determine if sodium chloride could impact the toxicity of the Ms-CFS against S. aureus NRS193 using 100 mM and 200 mM NaCl in 20% Ms-CFS or the media control.…”

Section: Sodium Chloride and Free Fatty Acids Exacerbate M Sympodiali...mentioning

confidence: 99%

Adaptation to skin mycobiota promotes antibiotic tolerance inStaphylococcus aureus

Kowalski,

Lawhorn,

Smith

et al. 2024

Preprint

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The microbiota can promote host health by inhibiting pathogen colonization, yet how host-resident fungi, or the mycobiota, contribute to this process remains unclear. The human skin mycobiota is uniquely stable compared to other body sites and dominated by yeasts of the genusMalassezia. We observe that colonization of human skin byMalassezia sympodialissignificantly reduces subsequent colonization by the prominent bacterial pathogenStaphylococcus aureus.M. sympodialissecreted products possess potent bactericidal activity againstS. aureusand are sufficient to impairS. aureusskin colonization. This bactericidal activity requires an acidic environment and is exacerbated by free fatty acids, demonstrating a unique synergy with host-derived epidermal defenses. Leveraging experimental evolution to pinpoint mechanisms ofS. aureusadaptation in response to the skin mycobiota, we identified multiple mutations in the stringent response regulator Rel that promote survival againstM. sympodialis. Similar Rel alleles have been reported inS. aureusclinical isolates, and natural Rel variants are sufficient for tolerance toM. sympodialisantagonism. Partial stringent response activation underlies tolerance to clinical antibiotics, with both laboratory-evolved and natural Rel variants conferring multidrug tolerance. These findings demonstrate the ability of the mycobiota to mediate pathogen colonization resistance, identify new mechanisms of bacterial adaptation in response to fungal antagonism, and reveal the potential for microbiota-driven evolution to shape pathogen antibiotic susceptibility.

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Sodium Chloride Promotes the Growth of Bacterial Soil Isolate and Antimicrobial Activity of Polymixin B

Singh

2024

Preprint

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The decomposition of organic matter in the soil, soil salinity, and soil acidity are influenced by soil microbiology, which also controls the recycling and processing of nutrients in the soil. These factors affect soil fertility and ecological stability. This manuscript is focused on the isolation of seven gram-negative bacteria found in the agricultural soil of The Neotia University campus, West Bengal, India. These isolates have been found to be mesophilic based on their study of their growth profiles, which revealed that under the same incubation conditions, the isolates displayed rising development patterns between 24 and 120 hours. The presence of sodium and potassium chloride modulates the growth and development of bacterial strains (DNI1, DNI2, DNI3, DNI4, DNI5, DNI6, and DNI7) during laboratory set up. The sodium and potassium chloride composition of culture media that effectively regulates the development of bacterial isolates has been determined using the combinational method of salt treatment. The ideal physical factor required for the growth and development of microorganisms has been demonstrated by the pH and temperature titration during this study. The powerful antibiotics known as polymyxin B, which are nonribosomal lipopeptides produced byPaenibacillus polymyxa, are especially effective against Gram-negative bacteria. Because multidrug-resistant Gram-negative bacteria have few other choices for treating infections, the use of polymyxins in clinical settings has increased despite their toxicity in the past. This study offers an update on the most current findings about the bioactivity of soil isolates and their significant relationships to temperature, pH, salt, and polymixin B sensitivity. The toxicity impact may be reduced by enhancing polymixin B’s antibacterial activity with salt treatment in clinical research.

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Sodium chloride augments the antibacterial activity of a novel penaeid shrimp-derived peptide (GPCR10) against halotolerant Staphylococcus aureus

Cited by 3 publications

References 63 publications

New Antibacterial Peptaibiotics against Plant and Fish Pathogens from the Deep-Sea-Derived Fungus Simplicillium obclavatum EIODSF 020

New Antibacterial Peptaibiotics against Plant and Fish Pathogens from the Deep-Sea-Derived Fungus Simplicillium obclavatum EIODSF 020

Adaptation to skin mycobiota promotes antibiotic tolerance inStaphylococcus aureus

Sodium Chloride Promotes the Growth of Bacterial Soil Isolate and Antimicrobial Activity of Polymixin B

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