Sodium Caseinate and Cholesterol Improve Bad Cooler Stallion Fertility

Campos, Guilherme; Garcia, Victor Filgueiras Cruz; Freitas-Dell’Aqua, C.P.; Segabinazzi, Lorenzo G.T.M.; Maciel, Luciana França Smith; Alvarenga, Marco Antônio; Papa, Frederico Ozanam; Dell’Aqua, J.A.

doi:10.1016/j.jevs.2020.103201

Cited by 7 publications

(5 citation statements)

References 24 publications

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“…Therefore, when evaluating the results presented in this work, it is proven that the replacement of egg yolk by casein, in the composition of the caprine semen freezing extender, does not cause a reduction in the quality of kinetics, plasma membrane integrity, acrosome integrity and MMP of spermatozoa submitted to freezing and thawing. Therefore, these results are in agreement with previous data obtained with bovine (Batellie et al, 2001;Diniz et al, 2020), horse (Campos et al, 2020) and sheep (Salgado et al, 2020), demonstrating that casein can be as effective in preserving the fertility parameters of frozen sperm as conventional extenders that have egg yolk in their composition.…”

Section: Parameterssupporting

confidence: 93%

Chemically defined diluent based on Tris-casein in freezing goat semen

Souza

Monteiro²,

Seal³

et al. 2022

RSD

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The objective of the present work was to elaborate on a chemically defined extender, based on Tris-casein, and to evaluate its efficacy in the goat semen cryopreservation. For this purpose, three goats were used. Eight ejaculates were collected per male (n = 24). After semen collection, the ejaculates were frozen in a conventional extender (Tris-egg yolk) – control group, as well as an extender based on Tris-casein, in different concentrations (CAS1: 0.125 g/l; CAS2: 0.25 g/l; CAS3: 0.5 g/l), combined with 5% of glycerol. After dilution, 200 x 106 sperm/ml was frozen in an automated system and stored in liquid nitrogen (−196 ºC). After thawing (37 ºC, 30 s), the samples were submitted to kinetic analysis (CASA) and functional analysis, through plasma membrane integrity, acrosome integrity and mitochondrial membrane potential. Regarding the kinetic parameters, plasma membrane integrity, acrosome integrity and mitochondrial membrane potential, there were no significant differences (P > 0.05) between the groups evaluated, with the exception of flagellar beat cross frequency (BCF), where the CAS3 group had a higher value (P ≤ 0.05) than the control group. It can be concluded that the chemically defined extender based on Tris-casein presents a viable alternative to the conventional diluent used for freezing goat semen.

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Section: Parameterssupporting

confidence: 93%

Chemically defined diluent based on Tris-casein in freezing goat semen

Souza

Monteiro²,

Seal³

et al. 2022

RSD

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“…The efflux of cholesterol from the plasma membrane is critical for sperm capacitation and fertilization ( 33 ), and excessive cholesterol levels in the sperm plasma membrane are well known to impair sperm capacitation and acrosome reaction ( 32 , 34 , 35 ). However, it is worth noting that the SM-based extender containing CLC used in the present study was previously tested in jacks ( 14 ), and both SM- and SC-based extenders containing CLC used in the present study were tested in horse stallions with no impairment to fertility ( 29 , 36 , 37 ). Therefore, it was reasonable to speculate that the extender containing CLC used in the present study would not impair the sperm capacitation process and fertility of cooled donkey semen.…”

Section: Discussionmentioning

confidence: 99%

Ability of donkey sperm to tolerate cooling: Effect of extender base and removal of seminal plasma on sperm parameters and fertility rates in mares

et al. 2022

Self Cite

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Artificial insemination using cooled-transported semen has marked importance in equine breeding programs around the world, and the high value of mules has generated avid interest in donkey semen biotechnology. However, donkey semen cools poorly in commercially available equine extenders. Therefore, this study aimed to develop approaches to improve the ability of donkey semen to tolerate cooling. Ejaculates of seven donkeys (n = 21) were cooled at 5°C for 48 h in three different extenders (milk-based, SM; sodium caseinate-based, SC; or egg yolk-based, EY) in the presence or absence of seminal plasma (centrifugation, C). Sperm motility, plasma membrane integrity (PMI), plasma membrane stability (PMS), mitochondrial membrane potential (HMMP), intracellular hydrogen peroxide (H2O2), and intracellular superoxide (O2−) were assessed before, 24 h, and 48 h post-cooling. In addition, 15 mares (163 estrous cycles) were randomly inseminated with semen from two jacks (Jack 1, n = 90; Jack 2, n = 73) previously cooled for 24 h under one of the treatments (SM, SC, EY, SM-C, SC-C, or EY-C). Groups EY, SC-C, and EY-C (P < 0.05) demonstrated superior sperm analytical parameters to SM at 24 and 48 h. Centrifugation positively affected sperm analytical parameters in cooled donkey semen extended in SM and SC (P < 0.05). Mares bred with semen extended in SC (67%, 18/27), SC-C (89%, 24/27), EY (89%, 25/28), or EY-C (74%, 20/27) had significantly greater conception rates than mares bred with SM (33%, 9/27; P < 0.05). Mares bred with SM-C had intermediate conception rates (59%, 16/27). In conclusion, SC and EY improved the cooling ability and fertility of donkey semen in horse mares, and centrifugation positively affected donkey semen extended in SM.

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“…Despite the promising results of including soy lecithin in BWW medium found in the present study, its efficacy is limited or controversial for semen cryopreservation, mainly in equine species (Papa et al, 2011, Caldevilla et al, 2020, as it has been shown to be inferior to other sources of macromolecules that function as externally acting cryoprotectants (Dalmazzo et al, 2019;Papa et al, 2011, Caldevilla et al, 2020, especially when compared to skim milk (Campos et al, 2020;Novello et al, 2020).…”

Section: Soymentioning

confidence: 71%

Sêmen equino refrigerado com diluidor quimicamente definido contendo lecitina de soja

Machado¹,

Brito

Santana

et al. 2023

Sem. Ci. Agr.

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O sêmen equino tem sido historicamente refrigerado usando meios à base de leite. No entanto, o uso de componentes de origem animal causa várias preocupações potenciais, como variabilidade nas formulações, contaminação microbiana e questões regulatórias. Objetivou-se avaliar o potencial de inclusão de diferentes concentrações de lecitina de soja (LS) no meio quimicamente definido BWW - Biggers, Whitten e Whittingham para refrigeração de sêmen equino e armazenamento na temperatura de 15°C. Os ejaculados foram diluídos em seis diferentes grupos experimentais: 1) BotuSêmen® (controle); 2) BWW; 3) BWW + 1% lecitina de soja (LS); 4) BWW + 2% LS; 5) BWW + 4% LS e 6) BWW + 6% LS. O meio BWW, não preservou a motilidade, a velocidade, a retilinearidade (STR), a linearidade (LIN), a amplitude do deslocamento lateral da cabeça (ALH), a frequência de batimento flagelar cruzado (BCF), a integridade funcional e estrutural dos espermatozoides equino durante 24 h de refrigeração quando comparado ao BotuSêmen® (P <0,05). O uso de BWW para refrigeração de sêmen equino só foi possível com adição de lecitina de soja, sendo as concentrações igual ou superior a 2% melhores, pois preservaram a motilidade total, a velocidade curvilinear (VCL) e LIN com mesmo potencial do BotuSêmen® (P >0,05). Ainda assim, o diluidor comercial BotuSêmen® apresentou superioridade em preservar o percentual de espermatozoides progressivamente móveis, a velocidade média da trajetória (VAP), a velocidade linear progressiva (VSL) e a frequência do batimento flagelar cruzado (BCF) durante a refrigeração comparado aos demais diluidores (P <0,05). A inclusão de lecitina de soja, de 2 a 6% no meio BWW, permitiu a manutenção da viabilidade do sêmen equino refrigerado a 15ºC por até 24 horas.

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Sodium Caseinate and Cholesterol Improve Bad Cooler Stallion Fertility

Cited by 7 publications

References 24 publications

Chemically defined diluent based on Tris-casein in freezing goat semen

Chemically defined diluent based on Tris-casein in freezing goat semen

Ability of donkey sperm to tolerate cooling: Effect of extender base and removal of seminal plasma on sperm parameters and fertility rates in mares

Sêmen equino refrigerado com diluidor quimicamente definido contendo lecitina de soja

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