Sodium Butyrate Inhibits the Enhancing Effect of High Fat Diet on Mammary Tumorigenesis

Abstract

We have studied the effect of butyrate on mammary tumorigenesis by 7,12-dimethylbenz(a)anthracene. As reported previously, a high incidence of mammary tumors was observed in rats fed a basal diet containing 20% margarine. However, the enhancing effect of margarine was inhibited when sodium butyrate was supplemented in the high margarine diet. Sodium butyrate did not cause any effect when it was supplemented in the basal diet. The result suggests a possibility that a part of the inhibitory effect of butter on mammary tumorigenesis, which we had previously reported, was caused by butyrate in milk lipids.

Milk intelligence: Mining milk for bioactive substances associated with human health

“…Here, butyrate is immediately absorbed, processed and released into the blood stream for transport to the liver where most is metabolized (Parodi, 1997;Smith, Yokoyama, & German, 1998). Interestingly, Yanagi, Yamashita, and Imai (1993) demonstrated that margarine supplemented with sodium butyrate significantly reduced the incidence of mammary tumors in a rat model. Ingestion of butyrate as a natural component of anhydrous milk fat (containing 0.8% butyrate) was found to be effective at inhibiting mammary tumorigenesis in rats (Belobrajdic & McIntosh, 2000).…”

Some Beneficial Effects of Probiotic Bacteria

“…Butyric acid is claimed to prevent carcinogenic effects at molecular (DNA) level (49,50,54). Yanagi et al (54) reported that addition of butyric acid to a diet containing 20% margarine prevented mammary tumour formation by 7,12-dimethylbenz(a) anthracene in rats.…”

“…Yanagi et al (54) reported that addition of butyric acid to a diet containing 20% margarine prevented mammary tumour formation by 7,12-dimethylbenz(a) anthracene in rats. Thus, it appears that antimutagenic effects of probiotic bacteria may be due to both inhibition by bacterial cells and production of organic acids, especially butyric acid.…”

Nutritional Implications of Replacing Bovine Milk Fat With Vegetable Oil in Infant Formulas

“…Based on extrapolations from rodent models, it is possible that the amount of CLA in milk fat (∼1%) may be sufficient to suppress mammary cancer and to lower cholesterol, although this has never been tested in humans. Health benefits have been attributed to butter in comparison with vegetable oils (55–59), but because vegetable oils and milk fat have many differences other than CLA, we cannot unequivocally conclude that CLA is the active ingredient in these studies.…”