Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis

Dou, Xiujing; Gao, Nan; Yan, Di; Shan, Anshan

doi:10.3390/ani10071154

Cited by 68 publications

(59 citation statements)

References 36 publications

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“…Due to colonic edema, the weight increased, the length shortened, and the ratio of colonic weight to colonic length was higher in the DSS model mice than that in normal mice. 1 , 19 , 20 The colonic length was significantly longer in the LA-G than in the DSS-G, and the ratio of colonic weight/length was smaller in the LA-G than in the DSS-G. The pathological sections of the colon tissue further showed that LA-XY27 treatment significantly reduced the infiltration and proliferation of lymphocytes caused by inflammation, which preliminarily proved the anti-inflammatory effect of LA-XY27 on DSS-induced colitis.…”

Section: Discussionmentioning

confidence: 80%

Preventive Effect of Lactobacillus acidophilus XY27 on DSS-Induced Ulcerative Colitis in Mice

Hu¹,

Wang²,

Xiang³

et al. 2020

DDDT

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Introduction Lactobacillus acidophilus is widely used as probiotic supplement in functional foods due to its beneficial regulatory effects on host, such as immune regulation, anti-inflammatory, and antioxidant activities. Aim This study aimed to determine the preventive effect of Lactobacillus acidophilus XY27 ( L. acidophilus XY27) on colitis induced by dextran sodium. Methods The mice were randomly divided into five groups. Except for the control group, the other four groups were induced for ulcerative colitis (UC) with dextran sodium sulfate (DSS), and three groups in DSS-groups were treated with L. acidophilus XY27, L. bulgaricus , and salicylazosulfapyridine. The weight change, DAI score, colon length, and length to weight ratio were tested. The oxidation index and the levels of inflammatory cytokines in the serum were measured. Subsequently, the gene expression levels of inflammatory factors in the colon tissue were determined by the Real-Time quantitative polymerase chain reaction (qRT-PCR) method. Results The results showed that the mice in the L. acidophilus XY27 group performed better in terms of weight, DAI score, colon length, and length to weight ratio or colonic pathological sections compared with the DSS-induced group. Further, the levels of tumor necrosis factor α (TNF-α), Interleukin-6 (IL-6), Interleukin-12 (IL-12) and Interleukin-1β (IL-1β), malondialdehyde (MDA) content, and myeloperoxidase activity in the serum of UC mice treated with L. acidophilus XY27 significantly decreased, while the levels of Interferon-γ (IFN-γ), Interleukin-10 (IL-10), Catalase (CAT), and total superoxide dismutase (SOD) significantly increased. The gene expression levels of Ets-like transcription factor-1 (EIK-1), IL-12, IL-1β, Cyclooxygenase 2 (COX-2), TNF-α, Escherichia coli, Lipopolysaccharide (LPS), and p100 in the colon significantly decreased while those of tight junction protein 1 (ZO-1), nuclear factor kappa B (NF-kB), p53, and NF-kappa-B inhibitor alpha (IκB-α) increased in the L. acidophilus XY27 group. Conclusion The results of the experiment suggested that L. acidophilus XY27 prevented colitis and alleviated symptoms in mice with DSS-induced UC, and also repaired the intestinal barrier function.

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Section: Discussionmentioning

confidence: 80%

Preventive Effect of Lactobacillus acidophilus XY27 on DSS-Induced Ulcerative Colitis in Mice

Hu¹,

Wang²,

Xiang³

et al. 2020

DDDT

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“…Mechanistically, SCFAs may promote the integrity of the intestinal epithelial barrier by increasing the synthesis of mucins in the mucosal layer [ 68 ] and of TJ proteins in the epithelial monolayer [ 69 , 70 ], and thus, contribute to the remission of colitis. In addition, SCFAs attenuate colitis in mice by restoring the balance of gut microbial dysbiosis [ 71 , 72 ]. In this study, feeding B. vulgatus 7K1 to mice protected the TJs of their intestinal epithelial cells ( Figure 7 ), but did not restore the balance of the gut microbial dysbiosis caused by DSS ( Figure 8 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Effects of Different Bacteroides vulgatus Strains against DSS-Induced Colitis

Wang

Zhang

et al. 2021

Journal of Immunology Research

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Although the strain-dependent effects of Bacteroides vulgatus on alleviating intestinal inflammatory diseases have been demonstrated, the literature has rarely focused on the underlying causes of this effect. In this study, we selected four B. vulgatus strains (FTJS5K1, FTJS7K1, FSDTA11B14, and FSDLZ51K1) with different genomic characteristics and evaluated their protective roles against dextran sulfate sodium- (DSS-) induced colitis. Compared to the other three tested strains, B. vulgatus 7K1 more strongly ameliorated the DSS-induced weight loss, shortening of the colon length, increased disease activity index scores, colonic tissue injury, and immunomodulatory disorder. In contrast, B. vulgatus 51K1 significantly worsened the DSS-induced alterations in the tumor necrosis factor-alpha (TNF-α) concentration and colonic histopathology. A comparative genomic analysis of B. vulgatus 7K1 and 51K1 showed that the beneficial effects of B. vulgatus 7K1 may be associated with some of its specific genes involved in the production of short-chain fatty acids or capsular polysaccharides and enhancement of its survivability in the gut. In conclusion, these findings indicate that the supplementation of B. vulgatus 7K1 is a potentially efficacious intervention for alleviating colitis and provides scientific support for the screening of probiotics with anticolitis effect.

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“…Nevertheless, butyrate does modulate intestinal inflammation, and dysbiosis in IBD may contribute to disease severity [ 70 , 71 ]. Butyrate mediates intestinal inflammation predominantly through binding of free fatty acid receptors (FFAR), a family of GPCR present on the epithelial cell membrane [ 72 , 73 , 74 ].…”

Section: Butyrate As An Immunomodulator In the Intestine And Beyondmentioning

confidence: 99%

Butyrate and the Intestinal Epithelium: Modulation of Proliferation and Inflammation in Homeostasis and Disease

Salvi

Cowles

2021

Cells

212

114

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The microbial metabolite butyrate serves as a link between the intestinal microbiome and epithelium. The monocarboxylate transporters MCT1 and SMCT1 are the predominant means of butyrate transport from the intestinal lumen to epithelial cytoplasm, where the molecule undergoes rapid β-oxidation to generate cellular fuel. However, not all epithelial cells metabolize butyrate equally. Undifferentiated colonocytes, including neoplastic cells and intestinal stem cells at the epithelial crypt base preferentially utilize glucose over butyrate for cellular fuel. This divergent metabolic conditioning is central to the phenomenon known as “butyrate paradox”, in which butyrate induces contradictory effects on epithelial proliferation in undifferentiated and differentiated colonocytes. There is evidence that accumulation of butyrate in epithelial cells results in histone modification and altered transcriptional activation that halts cell cycle progression. This manifests in the apparent protective effect of butyrate against colonic neoplasia. A corollary to this process is butyrate-induced inhibition of intestinal stem cells. Yet, emerging research has illustrated that the evolution of the crypt, along with butyrate-producing bacteria in the intestine, serve to protect crypt base stem cells from butyrate’s anti-proliferative effects. Butyrate also regulates epithelial inflammation and tolerance to antigens, through production of anti-inflammatory cytokines and induction of tolerogenic dendritic cells. The role of butyrate in the pathogenesis and treatment of intestinal neoplasia, inflammatory bowel disease and malabsorptive states is evolving, and holds promise for the potential translation of butyrate’s cellular function into clinical therapies.

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Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis

Cited by 68 publications

References 36 publications

Preventive Effect of Lactobacillus acidophilus XY27 on DSS-Induced Ulcerative Colitis in Mice

Preventive Effect of Lactobacillus acidophilus XY27 on DSS-Induced Ulcerative Colitis in Mice

Evaluation of the Effects of Different Bacteroides vulgatus Strains against DSS-Induced Colitis

Butyrate and the Intestinal Epithelium: Modulation of Proliferation and Inflammation in Homeostasis and Disease

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