Sodium azide mutagenesis generated diverse and broad spectrum blast resistance mutants in rice

Wang, Changsheng; Lo, Kuan-Lin; Wang, Arthur Z.

doi:10.1007/s10681-019-2468-4

Cited by 4 publications

(9 citation statements)

References 37 publications

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“…Our previous study showed that SA0169 conferred broad-spectrum resistance against all of the 89 Taiwanese blast isolates isolated during 1988–1991 (Wang et al 2019a ). SA0169 has been planted and showed resistance in our experimental field since ~ 20 years ago.…”

Section: Resultsmentioning

confidence: 99%

“…To map the genomic regions associated with the blast resistance in SA0169, a highly blast susceptible line LTH (Lijiangxintuanheigu), which showed susceptible responses to 175 of the 187 isolates, was crossed with SA0169 to establish the F 2 population for genetic analysis and mapping. A total of 122 F 2 offspring of LTH×SA0169 were challenged with the blast isolate MS2a2-1209 and the segregation of F 2 progenies fitted the 3:1 ratio of resistance to susceptibility, indicating that the blast resistance of SA0169 may be controlled by a single dominant locus ( χ 2 = 0.10, P = 0.75) (Wang et al 2019a ) (Additional file 2 : Table S2). To map the R loci of SA0169, a total of 1,102 simple sequence repeats (SSR) markers were applied to genotype the two parental lines, LTH and SA0169.…”

Section: Resultsmentioning

confidence: 99%

“…Chemical/physical mutagenesis is another way to develop new R genes/alleles that do not exist in natural germplasm, and can generate desirable resistant mutations that are free from the association of undesirable genetic changes (Khan et al 2009 ; Srivastava et al 2017 ). Several mutagenic agents, including diethyl sulphate (DES), ethylmethanesulfonate (EMS), N-methyl-N-nitrosourea (MNU), sodium azide (SA) (chemical mutagens), fast neutron, and γ-ray (physical mutagens), have been utilized to generate novel mutants for improving rice blast resistance (Wang et al 2019a ). Some mutants even had achieved prominent success, providing significant economic contributions (Ahloowalia et al 2004 ; Mohamad et al 2006 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, reported sodium azide induced rice blast resistant mutants are very limited, which include the Namil(SA)-bl5 mutant developed by National Institute of Crop Science (NICS) in Korea, and CRM 49 and CRM 51 mutants by ICAR-National Rice Research Institute in India (Cho et al 2014 ; Singh and Singh 2018 ). Our previous study identified 50 sodium azide induced blast resistant/susceptible mutants from a mutation pool of a japonica variety TNG67 (Wang et al 2019a ). This study suggested that sodium azide is a potent mutagen to induce sufficient mutations throughout the genome that can efficiently generate diverse and broad-spectrum blast resistant mutants in rice (Wang et al 2019a ).…”

Section: Introductionmentioning

confidence: 99%

“…Our previous study identified 50 sodium azide induced blast resistant/susceptible mutants from a mutation pool of a japonica variety TNG67 (Wang et al 2019a ). This study suggested that sodium azide is a potent mutagen to induce sufficient mutations throughout the genome that can efficiently generate diverse and broad-spectrum blast resistant mutants in rice (Wang et al 2019a ). To further identify valuable R genes in the sodium azide induced mutants for blast resistance improvement in breeding, one of the broad-spectrum blast resistant mutants, namely SA0169, was selected in this study.…”

Section: Introductionmentioning

confidence: 99%

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Two genomic regions of a sodium azide induced rice mutant confer broad-spectrum and durable resistance to blast disease

Chen²,

Chiang

et al. 2022

Rice

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Rice blast, one of the most destructive epidemic diseases, annually causes severe losses in grain yield worldwide. To manage blast disease, breeding resistant varieties is considered a more economic and environment-friendly strategy than chemical control. For breeding new resistant varieties, natural germplasms with broad-spectrum resistance are valuable resistant donors, but the number is limited. Therefore, artificially induced mutants are an important resource for identifying new broad-spectrum resistant (R) genes/loci. To pursue this approach, we focused on a broad-spectrum blast resistant rice mutant line SA0169, which was previously selected from a sodium azide induced mutation pool of TNG67, an elite japonica variety. We found that SA0169 was completely resistant against the 187 recently collected blast isolates and displayed durable resistance for almost 20 years. Linkage mapping and QTL-seq analysis indicated that a 1.16-Mb region on chromosome 6 (Pi169-6(t)) and a 2.37-Mb region on chromosome 11 (Pi169-11(t)) conferred the blast resistance in SA0169. Sequence analysis and genomic editing study revealed 2 and 7 candidate R genes in Pi169-6(t) and Pi169-11(t), respectively. With the assistance of mapping results, six blast and bacterial blight double resistant lines, which carried Pi169-6(t) and/or Pi169-11(t), were established. The complementation of Pi169-6(t) and Pi169-11(t), like SA0169, showed complete resistance to all tested isolates, suggesting that the combined effects of these two genomic regions largely confer the broad-spectrum resistance of SA0169. The sodium azide induced mutant SA0169 showed broad-spectrum and durable blast resistance. The broad resistance spectrum of SA0169 is contributed by the combined effects of two R regions, Pi169-6(t) and Pi169-11(t). Our study increases the understanding of the genetic basis of the broad-spectrum blast resistance induced by sodium azide mutagenesis, and lays a foundation for breeding new rice varieties with durable resistance against the blast pathogen.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Two genomic regions of a sodium azide induced rice mutant confer broad-spectrum and durable resistance to blast disease

Chen²,

Chiang

et al. 2022

Rice

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Investigation of genetic diversity on M2 mutant lines of Purslane (Portulaca oleracea) derived through DMS mutagenes

Fotokian

Kelasi

2020

jcb

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Identification of the CesA7 Gene Encodes Brittleness Mutation Derived from IR64 Variety and Breeding for Ruminant Feeding

Sawasdee,

Tsai,

Liao

et al. 2024

Agriculture

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Rice straw presents challenges as livestock feed due to its low digestibility and the presence of chemical residues. One potential solution is to focus on breeding brittle varieties that possess disease-resistance traits. In this study, AZ1803, a brittle mutant line isolated from the IR64 mutant pool, was chosen for gene identification and breeding. The AZ1803 mutant was crossed to the TNG67 variety to generate a mapping population and to the CS11 variety for fine mapping and breeding. The gene was mapped on chr. 10 between RM467 and RM171 SSR markers and was narrowed down to RM271 and RM5392 with 600 kb proximately interval. The AZ1803 and IR64 sequencing results revealed a substitution mutant in the Exon 9th of the OsCesA7 gene, resulting in an amino acid mutation at the end of the transmembrane domain 5th of the CESA7, responsible for cellulose synthesis for the secondary cell wall. The cellulose content of AZ1803 was reduced by 25% compared with the IR64. A new brittle and disease-resistant variety was bred by using developed markers in marker-assisted selection. In addition, bending tests and bacterial blight inoculation were applied. The bacterial lesion length of the bred variety is 64% lower than that of AZ1803. The rice straw of the new variety can be used for livestock feeding, which increases farmer income and reduces pesticide residues and air pollution from straw burning.

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Sodium azide mutagenesis generated diverse and broad spectrum blast resistance mutants in rice

Cited by 4 publications

References 37 publications

Two genomic regions of a sodium azide induced rice mutant confer broad-spectrum and durable resistance to blast disease

Two genomic regions of a sodium azide induced rice mutant confer broad-spectrum and durable resistance to blast disease

Investigation of genetic diversity on M2 mutant lines of Purslane (Portulaca oleracea) derived through DMS mutagenes

Identification of the CesA7 Gene Encodes Brittleness Mutation Derived from IR64 Variety and Breeding for Ruminant Feeding

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