Sodium and potassium fluxes in cells cultured from chick embryo heart muscle

Burrows, Robert B.; Lamb, J. F.

doi:10.1113/jphysiol.1962.sp006947

Cited by 42 publications

(22 citation statements)

References 23 publications

(23 reference statements)

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“…In frog skeletal muscle the rate of Na efflux appeared to be dependent upon the [Na]? (Keynes & Swan, 1959) but in monolayer cultured chick embryo heart cells the rate of efflux was linearly related to the [Na]i (Burrows & Lamb, 1962).…”

Section: The Effect Of External Na and Camentioning

confidence: 99%

The effects of external cations and ouabain on the intracellular sodium activity of sheep heart Purkinje fibres

Ellis¹

1977

The Journal of Physiology

313

219

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SUMMARY1. The intracellular Na activity of sheep heart Purkinje fibres has been measured using recessed-tip Na+-sensitive glass micro-electrodes.2. The internal Na activity was 7-2 + 2-0 mm (mean + S.D., n = 32) at the normal external Na concentration, [Na]0, in these experiments of 140 mm (equivalent to an external Na activity of 105 mM). The equilibrium potential for Na across the fibre membrane was therefore approximately + 70 mV. 4. The removal of external K produced an easily reversible increase in the internal Na with an initial rate equivalent to a concentration change of 0-24 + 0 07 m-mole/min (mean + S.D., n = 8).5. Ouabain produced increases in the internal Na activity that were only very slowly reversible. The threshold concentration for producing an increase was approximately 10-7 M. 10. The relationship between internal Na and contractility is discussed.

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Section: The Effect Of External Na and Camentioning

confidence: 99%

The effects of external cations and ouabain on the intracellular sodium activity of sheep heart Purkinje fibres

Ellis¹

1977

The Journal of Physiology

313

219

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“…For L cells, this method of measuring cell volume depends on the assumption that trypsinization does not greatly alter the volume of the cell but merely the shape; this problem has been discussed in an earlier paper (Burrows & Lamb, 1962). To check this assumption, the cell volume w-as also measured by considering L cells as 'right prisms' and as 'oblate spheroids' (i.e.…”

Section: Culture Methodsmentioning

confidence: 99%

“…Burrows & Lamb (1962) calculated that the membrane of cells next to the supporting surface was available for ion transfer, but Borle (1969) has recently assumed this was not so. During the course of the present work, numerous E.M. pictures were taken of L cells showing the space between them and the supporting medium.…”

Section: Estimation Of Nucleotides In L Cellsmentioning

confidence: 99%

“…It has been found that the cells from various tissues can be cultured as a monolayer in cell culture, a state which allows of relatively easy measurement of Na pumping (Burrows & Lamb, 1962). The present experiments were designed to measure the Na and K transport properties in a detailed way in one such cell line (L cells derived from mouse subcutaneous tissue), primarily for comparison with the findings in R.B.C.s and squid axon, but also to provide a basis for work in progress on adaptation in cultured cells.…”

Section: Introductionmentioning

confidence: 99%

“…The present experiments were designed to measure the Na and K transport properties in a detailed way in one such cell line (L cells derived from mouse subcutaneous tissue), primarily for comparison with the findings in R.B.C.s and squid axon, but also to provide a basis for work in progress on adaptation in cultured cells. The methods used are largely based on earlier work (Burrows & Lamb, 1962); preliminary accounts of the results have appeared (Lamb & MacKinnon, 1967).…”

Section: Introductionmentioning

confidence: 99%

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Effect of ouabain and metabolic inhibitors on the Na and K movements of nucleotide contents of L cells

Lamb¹,

Mackinnon²

1971

The Journal of Physiology

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SUMMARY1. The general characteristics and Na and K movements of L cells (derived from mouse epithelium) have been measured. Both cells grown in suspension (LS cells) and as a monolayer (L cells) were used.2. The volume of L cells was 1-2 x 10-9 cm3 and of LS cells 3-5 x 10-9 cm3; of this 82 % was water.3. Electron micrographs showed the presence of numerous protrusions (filopodia) from both forms of the cell. These had the effect of increasing the surface area of the cell by 2-4 times over smooth cells of the same volume. On changing from the flattened to the spherical shape during trypsinization, the filopodia altered to maintain a constant V/A ratio. 6. Both DNP (10-3M) and IAA (IO 4M) are required for maximum inhibition of K uptake, as both aerobic and anaerobic metabolic pathways may be used to drive the pump.7. K removal decreases the Na efflux, and Na removal (eventually) decreases the K influx providing evidence for Na/K coupling.

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Response of cultured chick heart cells to changes in ionic environment

Levinson

Green

1966

Journal Cellular Physiology

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Ventricles from 11-day-old chick embryonic heart were disaggregated by elastase and the component cells cultured on glass in maintenance medium containing 10 pc of P3'. After 48 hours incubation at 37°C the medium was removed, the cells rinsed and exposed to a phosphate-free test solution for two hours. During this period samples of the test medium were removed for counting and spectrophotometric analysis. Cells, incubated in solutions lacking amino acids or vitamins or serum components lost phosphate at essentially the same rate as in the complete culture medium; furthermore such cells lost very small amounts of nucleotide materials. Cells incubated in 0.16 M NaCl lost phosphate and nucleotides rapidly; the addition of either K+ or Ca+2 or MgfZ reduced phosphate and nucleotide loss and cells in balanced saline media containing all four cations, retained phosphate and nucleotides at essentially the same level as in the complete medium. These results show that primary isolated chick heart cells can be maintained for short periods in physiological saline solutions without injury and that saline balance in short term studies is a primary factor in maintaining these cells in an uninjured state.

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Sodium and potassium fluxes in cells cultured from chick embryo heart muscle

Cited by 42 publications

References 23 publications

The effects of external cations and ouabain on the intracellular sodium activity of sheep heart Purkinje fibres

The effects of external cations and ouabain on the intracellular sodium activity of sheep heart Purkinje fibres

Effect of ouabain and metabolic inhibitors on the Na and K movements of nucleotide contents of L cells

Response of cultured chick heart cells to changes in ionic environment

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