Sodalis ligni Strain 159R Isolated from an Anaerobic Lignin-Degrading Consortium

Chaput, Gina; Ford, Jacob; DeDiego, Lani; Narayanan, Achala; Tam, Wing Yin; Whalen, Meghan; Clum, Alicia; Spunde, Alex; Pillay, Manoj; Palaniappan, Krishnaveni; Varghese, Neha; Mikhailova, Natalia; Chen, I-Min A.; Stamatis, Dimitrios; Reddy, T. B. K.; O’Malley, Ronan; Daum, Chris; Shapiro, Nicole; Ivanova, Natalia; Kyrpides, Nikos; Woyke, Tanja; Rio, Tijana Glavina del; DeAngelis, Kristen M.

doi:10.1128/spectrum.02346-21

Cited by 4 publications

(1 citation statement)

References 70 publications

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“…Tolumonas lignolytica BRL6-1T harbors various lignin degradation pathways; hence, it could enzymatically cleave the β-aryl ether bonds, depolymerizes lignin through extracellular peroxidases, and breaks down lignin-derived aromatic compounds [16]. Chaput et al isolated a novel anaerobic lignin-degrading species, Sodalis ligni, from forest soil, and also found a series of lignin and aromatic compound degradation/metabolism enzymes in its genome [17]. Despite the knowledge of the genomic information of these bacteria, the anaerobic degrading mechanism of lignin is still not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Lignin Degradation by Klebsiella aerogenes TL3 under Anaerobic Conditions

Tu,

Geng,

Xiang

et al. 2024

Molecules

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Lignin, the largest non-carbohydrate component of lignocellulosic biomass, is also a recalcitrant component of the plant cell wall. While the aerobic degradation mechanism of lignin has been well-documented, the anaerobic degradation mechanism is still largely elusive. In this work, a versatile facultative anaerobic lignin-degrading bacterium, Klebsiella aerogenes TL3, was isolated from a termite gut, and was found to metabolize a variety of carbon sources and produce a single kind or multiple kinds of acids. The percent degradation of alkali lignin reached 14.8% under anaerobic conditions, and could reach 17.4% in the presence of glucose within 72 h. Based on the results of infrared spectroscopy and 2D nuclear magnetic resonance analysis, it can be inferred that the anaerobic degradation of lignin may undergo the cleavage of the C-O bond (β-O-4), as well as the C-C bond (β-5 and β-β), and involve the oxidation of the side chain, demethylation, and the destruction of the aromatic ring skeleton. Although the anaerobic degradation of lignin by TL3 was slightly weaker than that under aerobic conditions, it could be further enhanced by adding glucose as an electron donor. These results may shed new light on the mechanisms of anaerobic lignin degradation.

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Section: Introductionmentioning

confidence: 99%