Social status differences regulate the serotonergic system of a cichlid fish, <i>Astatotilapia burtoni</i>

Loveland, Jasmine Lopez; Uy, Natalie; Maruska, Karen P.; Carpenter, Russ E.; Fernald, Russell D.

doi:10.1242/jeb.100685

Cited by 44 publications

(41 citation statements)

References 82 publications

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“…Of note, three genes related to sex differentiation in fishes ( parm1 [80], 5-hydroxytryptamine receptor 1A-beta-like [81], and gadd45gamma [82]) and one to sex-linked behavior ( 5ht receptor 1a [81]) were found within 120 kb of each other on one of the short distal scaffolds (scaffold 185; S7 Table). …”

Section: Resultsmentioning

confidence: 99%

The Genome of the Trinidadian Guppy, Poecilia reticulata, and Variation in the Guanapo Population

et al. 2016

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For over a century, the live bearing guppy, Poecilia reticulata, has been used to study sexual selection as well as local adaptation. Natural guppy populations differ in many traits that are of intuitively adaptive significance such as ornamentation, age at maturity, brood size and body shape. Water depth, light supply, food resources and predation regime shape these traits, and barrier waterfalls often separate contrasting environments in the same river. We have assembled and annotated the genome of an inbred single female from a high-predation site in the Guanapo drainage. The final assembly comprises 731.6 Mb with a scaffold N50 of 5.3 MB. Scaffolds were mapped to linkage groups, placing 95% of the genome assembly on the 22 autosomes and the X-chromosome. To investigate genetic variation in the population used for the genome assembly, we sequenced 10 wild caught male individuals. The identified 5 million SNPs correspond to an average nucleotide diversity (π) of 0.0025. The genome assembly and SNP map provide a rich resource for investigating adaptation to different predation regimes. In addition, comparisons with the genomes of other Poeciliid species, which differ greatly in mechanisms of sex determination and maternal resource allocation, as well as comparisons to other teleost genera can begin to reveal how live bearing evolved in teleost fish.

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Section: Resultsmentioning

confidence: 99%

The Genome of the Trinidadian Guppy, Poecilia reticulata, and Variation in the Guanapo Population

et al. 2016

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“…Among the various receptor subtypes that mediate 5HT function, the htr2a receptors have been shown to directly affect reproductive behaviour when manipulated in fish (Smith & Combs ), and higher mRNA levels are found in the telencephalon of subordinate males compared to dominant ones (Loveland et al . ). The htr2b receptor is expressed in several organs in puffer fish ( Tetraodon fluviatilis ), including in the heart and brain (De Lucchini et al .…”

Section: Discussionmentioning

confidence: 97%

“…We calculated the turnover rate using the ratio of concentration of metabolites on the concentration of the monoamine (Winberg et al 1991). A high ratio is indicative of elevated catabolic activity and has been interpreted as a high monoamine demand in the brain (Elipot et al 2014;Loveland et al 2014).…”

Section: Brain Monoaminesmentioning

confidence: 99%

“…This suggests that the specific functions and signalling associated with these receptor subtypes could be affected in opposite directions in freshwater and marine individuals. Among the various receptor subtypes that mediate 5HT function, the htr2a receptors have been shown to directly affect reproductive behaviour when manipulated in fish (Smith & Combs 2008), and higher mRNA levels are found in the telencephalon of subordinate males compared to dominant ones (Loveland et al 2014). The htr2b receptor is expressed in several organs in puffer fish (Tetraodon fluviatilis), including in the heart and brain (De Lucchini et al 2001), although its function in fish brain remains unclear.…”

Section: Functional Implications Of the Differential Regulation Of Prnsmentioning

confidence: 99%

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Receptors rather than signals change in expression in four physiological regulatory networks during evolutionary divergence in threespine stickleback

et al. 2016

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The molecular mechanisms underlying behavioural evolution following colonization of novel environments are largely unknown. Molecules that interact to control equilibrium within an organism form physiological regulatory networks. It is essential to determine whether particular components of physiological regulatory networks evolve or if the network as a whole is affected in populations diverging in behavioural responses, as this may affect the nature, amplitude and number of impacted traits. We studied the regulation of four physiological regulatory networks in freshwater and marine populations of threespine stickleback raised in a common environment, which were previously characterized as showing evolutionary divergence in behaviour and stress reactivity. We measured nineteen components of these networks (ligands and receptors) using mRNA and monoamine levels in the brain, pituitary and interrenal gland, as well as hormone levels. Freshwater fish showed higher expression in the brain of adrenergic (adrb2a), serotonergic (htr2a) and dopaminergic (DRD2) receptors, but lower expression of the htr2b receptor. Freshwater fish also showed higher expression of the mc2r receptor of the glucocorticoid axis in the interrenals. Collectively, our results suggest that the inheritance of the regulation of these networks may be implicated in the evolution of behaviour and stress reactivity in association with population divergence. Our results also suggest that evolutionary change in freshwater threespine stickleback may be more associated with the expression of specific receptors rather than with global changes of all the measured constituents of the physiological regulatory networks.

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“…6B). These same regions contain serotonergic neurons in this species (Loveland et al, 2014), but double-label studies are needed to determine whether vglut3 is coexpressed in serotonergic cells. In these same sections, a small population of vglut3-expressing cells is found lateral and ventral to the SR (arrows in Fig.…”

Section: Vglut3mentioning

confidence: 99%

Localization of glutamatergic, GABAergic, and cholinergic neurons in the brain of the African cichlid fish, Astatotilapia burtoni

Maruska

Butler

Field

et al. 2016

J of Comparative Neurology

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Neural communication depends on release and reception of different neurotransmitters within complex circuits that ultimately mediate basic biological functions. We mapped the distribution of glutamatergic, GABAergic, and cholinergic neurons in the brain of the African cichlid fish Astatotilapia burtoni using in situ hybridization to label vesicular glutamate transporters (vglut1, vglut2.1, vglut3), glutamate decarboxylases (gad1, gad2), and choline acetyltransferase (chat). Cells expressing the glutamatergic markers vgluts 1-3 show primarily nonoverlapping distribution patterns, with the most widespread expression observed for vglut2.1, and more restricted expression of vglut1 and vglut3. vglut1 is prominent in granular layers of the cerebellum, habenula, preglomerular nuclei, and several other diencephalic, mesencephalic, and rhombencephalic regions. vglut2.1 is widely expressed in many nuclei from the olfactory bulbs to the hindbrain, while vglut3 is restricted to the hypothalamus and hindbrain. GABAergic cells show largely overlapping gad1 and gad2 expression in most brain regions. GABAergic expression dominates nuclei of the subpallial ventral telencephalon, while glutamatergic expression dominates nuclei of the pallial dorsal telencephalon. chat-expressing cells are prominent in motor cranial nerve nuclei, and some scattered cells lie in the preoptic area and ventral part of the ventral telencephalon. A localization summary of these markers within regions of the conserved social decision-making network reveals a predominance of either GABAergic or glutamatergic cells within individual nuclei. The neurotransmitter distributions described here in the brain of a single fish species provide an important resource for identification of brain nuclei in other fishes, as well as future comparative studies on circuit organization and function. J. Comp. Neurol. 525:610-638, 2017. © 2016 Wiley Periodicals, Inc.

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Social status differences regulate the serotonergic system of a cichlid fish, Astatotilapia burtoni

Cited by 44 publications

References 82 publications

The Genome of the Trinidadian Guppy, Poecilia reticulata, and Variation in the Guanapo Population

The Genome of the Trinidadian Guppy, Poecilia reticulata, and Variation in the Guanapo Population

Receptors rather than signals change in expression in four physiological regulatory networks during evolutionary divergence in threespine stickleback

Localization of glutamatergic, GABAergic, and cholinergic neurons in the brain of the African cichlid fish, Astatotilapia burtoni

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