Social Self‐Sorting of Colloidal Families in Co‐Assembling Microgel Systems

Han, Kang; Go, Dennis; Tigges, Thomas; Rahimi, Khosrow; Kuehne, Alexander J. C.; Walther, Andreas

doi:10.1002/ange.201612196

Cited by 30 publications

(11 citation statements)

References 49 publications

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“…For example, Walther and co-workers demonstrated the social-sorting into two families of colloids based on the orthogonal interactions of αcyclodextrin with azobenzenes and β-cyclodextrin with ferrocene. 33 Social self-sorting enables the formation of order in complex mixtures of colloids and hence will also enable the assembly of minimal synthetic cells into prototissues with high complexity. In this work, we aim to achieve the social selfsorting between two families of colloids as models to be used for minimal synthetic cells with noninvasive visible light under biocompatible conditions.…”

mentioning

confidence: 99%

Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins

Chervyachkova

Wegner

2018

ACS Synth. Biol.

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Toward the bottom-up assembly of synthetic cells from molecular building blocks, it is an ongoing challenge to assemble micrometer sized compartments that host different processes into precise multicompartmental assemblies, also called prototissues. The difficulty lies in controlling interactions between different compartments dynamically both in space and time, as these interactions determine how they organize with respect to each other and how they work together. In this study, we have been able to control the self-assembly and social self-sorting of four different types of colloids, which we use as a model for synthetic cells, into two separate families with visible light. For this purpose we used two photoswitchable protein pairs (iLID/Nano and nHagHigh/pMagHigh) that both reversibly heterodimerize upon blue light exposure and dissociate from each other in the dark. These photoswitchable proteins provide noninvasive, dynamic, and reversible remote control under biocompatible conditions over the self-assembly process with unprecedented spatial and temporal precision. In addition, each protein pair brings together specifically two different types of colloids. The orthogonality of the two protein pairs enables social self-sorting of a four component mixture into two distinct families of colloidal aggregates with controlled arrangements. These results will ultimately pave the way for the bottom-up assembly of multicompartment synthetic prototissues of a higher complexity, enabling us to control precisely and dynamically the organization of different compartments in space and time.

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mentioning

confidence: 99%

Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins

Chervyachkova

Wegner

2018

ACS Synth. Biol.

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“…ATP-driven alternating DNA polymers. Two dsDNA monomers, A (D 15 and D 16 ) and B (D 17 and D 18 ) were used to make transiently alternating DNA copolymer ( Supplementary Fig. 4a).…”

Section: Methodsmentioning

confidence: 99%

“…8a). Briefly, 10.0 μM Cy3-labeled dsDNA tiles annealed from D 18 and D 26 , 10.0 μM spacer tile (dsDNA from D 15 and D 16 or dsDNA from D 33 and D 34 ), 12.0 μM ssDNA-branch (D 29 ), and 0.92 WU μL −1 T4 DNA ligase were dissolved in 1× NEB CutSmart buffer. Then, 0.2 mM ATP was added to start the polymerization and the system was kept running at 37°C for 12 h. The SfNAPs were characterized by AGE (see above).…”

Section: Static Colloid Assembly By Branched Sfnaps With Variable Bramentioning

confidence: 99%

“…Equilibrium self-assembly from diverse building blocks allows complex hierarchical structures in solution and bulk, and encoding switchability leads to responsive materials such as in structural, optical or bioactive materials for actuators and soft robotics 3 , 11 – 14 . A recent extension in equilibrium self-assembly was the move towards self-sorting multicomponent systems, most notably from the peptide and colloidal self-assembly fields 15 , 16 .…”

Section: Introductionmentioning

confidence: 99%

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ATP-powered molecular recognition to engineer transient multivalency and self-sorting 4D hierarchical systems

Deng

Walther

2020

Nat Commun

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Biological systems organize multiple hierarchical structures in parallel, and create dynamic assemblies and functions by energy dissipation. In contrast, emerging artificial nonequilibrium self-assembling systems have remained relatively simplistic concerning hierarchical design, and non-equilibrium multi-component systems are uncharted territory. Here we report a modular DNA toolbox allowing to program transient non-equilibrium multicomponent systems across hierarchical length scales by introducing chemically fueled molecular recognition orchestrated by reaction networks of concurrent ATP-powered ligation and cleavage of freely programmable DNA building blocks. Going across hierarchical levels, we demonstrate transient side-chain functionalized nucleic acid polymers, and further introduce the concept of transient cooperative multivalency as a key to bridge length scales to pioneer fuel-driven encapsulation, self-assembly of colloids, and non-equilibrium transient narcissistic colloidal self-sorting on a systems level. The fully programmable and functionalizable DNA components pave the way to design chemically fueled 4D (3 space, 1 time) molecular multicomponent systems and autonomous materials.

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“…We profit from this specific selectivity at the molecular level, to prepare two-component dispersions of silica microparticles, which spontaneously self-sort into single-specie clusters upon UV-illumination, hence increasing the generality and modularity of our approach. Interestingly, colloidal gels with this arrested complex architecture 7 , 37 – 40 and other similar illustrative examples 33 , 41 have shown promising mechanical properties and could serve as a model to understand biological phenomena. The exploitation of our approach based on the high specificity and fidelity of H-bonding supramolecular moieties, has resulted for the first time in a light-activated colloidal self-sorting of identical particles into single-species clusters.…”

Section: Introductionmentioning

confidence: 94%

Light induced assembly and self-sorting of silica microparticles

Vilanova

Feijter

Teunissen

et al. 2018

Sci Rep

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To tailor the properties of colloidal materials, precise control over the self-assembly of their constituents is a prerequisite. Here, we govern the assembly of silica particles by functionalization with supramolecular moieties which interact with each other via directional and reversible hydrogen bonding. Through a generally applicable synthesis protocol, two different types of self-complementary hydrogen bonding moieties, BTA-and UPy-derivatives, are anchored to silica particles. Their selfassembly is initiated by the UV-induced removal of a photolabile protecting group, allowing the formation of hydrogen bonds between tethered molecules. The light-induced assembly of BTA-and UPy-decorated colloids in single-component dispersions and colloidal self-sorting in mixed dispersions is studied. Furthermore, we demonstrate that UPy-colloids can dissasemble upon addition of traces of a competitive binder (NaPy). This work provides further insight into the utility of supramolecular handles to orchestrate the assembly of micron-sized colloids via non-oligonucleotide hydrogen-bonding units.The ability to control molecular self-assembly via non-covalent interactions is an emerging bottom-up strategy to create complex metamaterials. In turn, well-defined molecular recognition can be used as a powerful tool to direct the assembly of micron-sized 1-3 colloids or even macroscopic objects 4 and create larger aggregated structures with emergent optical 5-7 , mechanical 8 or catalytic properties 9,10 . Molecular control over the assembly of micron-sized particles can be realized through particle surface-grafting of molecules exhibiting dipole-dipole 11,12 , metal coordination 13 , hydrophobic forces 14,15 and/or hydrogen bonding (H-bonding) [16][17][18] . Ideally, the tethered motifs should be easy to synthesize, responsive, externally addressable and provide directional, dynamic and specific bonding within the assemblies. Moreover, to facilitate colloidal assembly, a precise control between the interplay of non-specific surface forces (attractive and/or repulsive) is crucial 19 .A popular strategy in the field is the functionalization of colloids with complementary DNA strands 20,21 . Great advances in insight and structural complexity have been achieved in the last 20 years, enabling the assembly of colloids into both disordered and exotic ordered states 22,23 , in a reversible 22,24,25 or irreversible 26 manner and at the nano-27,28 and mesoscale 25,29 . Notwithstanding, DNA-technology has its limitations, such as high cost and restricted environmental conditions 27,30 because the strands only assemble in water at sufficiently high ionic strengths and at low temperature, precluding material synthesis and processing from organic solvents. Furthermore, up to now, only temperature has been used as external trigger of assembly, while recent advances towards light sensitive DNA-strands can be utilized in future to develop dual-responsive systems sensitive to both temperature and light 31 . A promising alternative strategy exploits...

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Social Self‐Sorting of Colloidal Families in Co‐Assembling Microgel Systems

Cited by 30 publications

References 49 publications

Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins

Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins

ATP-powered molecular recognition to engineer transient multivalency and self-sorting 4D hierarchical systems

Light induced assembly and self-sorting of silica microparticles

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