Social rank-dependent effects of testosterone on huddling strategies in mice

Zhao, Ye; Yin, Xueyong; Yu, Yang; Huang, Shihao; Feng, Hao; Wang, Shuang; Cai, Yao; Zhao, Ruofan; Gao, Shuai; Liu, Ye; Zhang, Zhengxin; Zhao, Xinyu; Qiao, Ruonan; Yuan, Jialin; Guan, Weichen; Yang, Huanming; Shi, Yun; Shi, Haishui

doi:10.1016/j.isci.2023.106516

Cited by 6 publications

(3 citation statements)

References 95 publications

(196 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, this alteration is not of toxicological concern, as the observed P levels remained within the normal range as defined by Frye et al [ 42 ]. Similarly, T levels showed a significant reduction in male mice at 55 mg PTSO/kg b.w./day when compared with the control groups ( p < 0.05), although these levels were within a normal range [ 43 ]. In contrast, female mice showed no significant alteration in T levels.…”

Section: Resultsmentioning

confidence: 99%

Two-Generation Toxicity Study of the Antioxidant Compound Propyl-Propane Thiosulfonate (PTSO)

Cascajosa-Lira,

Guzmán-Guillén,

Pichardo

et al. 2024

Antioxidants

View full text Add to dashboard Cite

Propyl-propane thiosulfonate (PTSO), an antioxidant organosulfur compound present in the genus Allium, has become a potential natural additive for food and feed, as well as a possible biopesticide for pest control in plants. A toxicological assessment is necessary to verify its safety for livestock, consumers, and the environment. As part of the risk assessment of PTSO, this study was designed to explore its potential reproductive toxicity in mice following the OECD 416 guideline. The investigation spans two generations to comprehensively evaluate potential reproductive, teratogenic, and hereditary effects. A total of 80 CD1 mice per sex and generation were subjected to PTSO exposure during three phases (premating, gestation, and lactation). This evaluation encompassed three dose levels: 14, 28, and 55 mg PTSO/kg b.w./day, administered through the feed. No clinical changes or mortality attributed to the administration of PTSO were observed in the study. Some changes in the body weight and food consumption were observed, but not related to sex or in a dose-dependent manner. The two parental generations (F0, F1) exhibited normal reproductive performance, and the offspring (F1 and F2) were born without any abnormalities. The serum sexual hormone levels (progesterone -P-, testosterone -T-, estradiol -E2-, follicular stimulating hormone -FSH-, and luteinizing hormone -LH-) were in a normal range. Although significant changes were observed in the sperm analysis in the case of F0 group, no variation was found for F1 group, and no alterations in fertility were recorded either. The absolute organ weights and relative organ weight/body weight and organ weight/brain weight ratios, and the complete histopathological study, showed no significant alterations in males and females for all the generations considered. Considering all the results obtained, PTSO is not considered a reproductive or developmental toxicant in mice under the assayed conditions. These results support the good safety profile of PTSO for its potential application in the agrifood sector.

show abstract

Section: Resultsmentioning

confidence: 99%

Two-Generation Toxicity Study of the Antioxidant Compound Propyl-Propane Thiosulfonate (PTSO)

Cascajosa-Lira,

Guzmán-Guillén,

Pichardo

et al. 2024

Antioxidants

View full text Add to dashboard Cite

show abstract

“…These include dyadic motifs (such as nose-to-nose contacts) and individual motifs (such as climbing), which are reported individually for all tracked mice. The stopped-and-huddled classifier 28 is abbreviated as “huddle” in DeepOF output (not to be confused with group huddling behavior 67 ). D Schematic representation of the supervised pipeline in DeepOF.…”

Section: Introductionmentioning

confidence: 99%

Automatically annotated motion tracking identifies a distinct social behavioral profile following chronic social defeat stress

et al. 2023

View full text Add to dashboard Cite

Severe stress exposure increases the risk of stress-related disorders such as major depressive disorder (MDD). An essential characteristic of MDD is the impairment of social functioning and lack of social motivation. Chronic social defeat stress is an established animal model for MDD research, which induces a cascade of physiological and behavioral changes. Current markerless pose estimation tools allow for more complex and naturalistic behavioral tests. Here, we introduce the open-source tool DeepOF to investigate the individual and social behavioral profile in mice by providing supervised and unsupervised pipelines using DeepLabCut-annotated pose estimation data. Applying this tool to chronic social defeat in male mice, the DeepOF supervised and unsupervised pipelines detect a distinct stress-induced social behavioral pattern, which was particularly observed at the beginning of a novel social encounter and fades with time due to habituation. In addition, while the classical social avoidance task does identify the stress-induced social behavioral differences, both DeepOF behavioral pipelines provide a clearer and more detailed profile. Moreover, DeepOF aims to facilitate reproducibility and unification of behavioral classification by providing an open-source tool, which can advance the study of rodent individual and social behavior, thereby enabling biological insights and, for example, subsequent drug development for psychiatric disorders.

show abstract

“…However, grouping behavior can be broadly defined as any type of behavior that occurs in groups and can focus not only on the group as a whole unit but also on individuals within the group (Krause and Ruxton, 2002;Majolo and Huang, 2022). Behaviors that occur in groups may also occur in non-group environments, such as foraging, which can occur alone or with others, or huddling which can occur in dyadic or large-group interactions (Gobrogge and Wang, 2015;Ding et al, 2020;Zhao et al, 2023). Importantly, though, the social context of being in a group may be quite distinct from that of the social context of being with just one other individual.…”

Section: Introductionmentioning

confidence: 99%

Advancements in the study of neural mechanisms underlying mammalian grouping behaviour

Kelly

2023

Front. Ethol.

View full text Add to dashboard Cite

Despite the prevalence of large group-living in the animal kingdom, we know surprisingly little about how the brain facilitates grouping behavior, particularly in mammals. In this brief communication, I provide an update on advancements in the study of the neural mechanisms underlying mammalian grouping behavior. I discuss the benefits of using non-traditional organisms in the laboratory and provide examples of how using non-standard, large housing and testing apparatuses produces more ethologically-relevant behavioral datasets. Further, with advancements in computer vision-based automated tracking and increasing availability of wireless neural recording and manipulation tools, scientists can now generate unprecedented neurobehavioral datasets from multiple interacting animals. Together, recent advancements in behavioral and neural approaches hold great promise for expanding our understanding of how the brain modulates complex, mammalian grouping behaviors.

show abstract

Social rank-dependent effects of testosterone on huddling strategies in mice

Cited by 6 publications

References 95 publications

Two-Generation Toxicity Study of the Antioxidant Compound Propyl-Propane Thiosulfonate (PTSO)

Two-Generation Toxicity Study of the Antioxidant Compound Propyl-Propane Thiosulfonate (PTSO)

Automatically annotated motion tracking identifies a distinct social behavioral profile following chronic social defeat stress

Advancements in the study of neural mechanisms underlying mammalian grouping behaviour

Contact Info

Product

Resources

About