Perceptual sociophonetic work on Guatemalan Spanish has demonstrated that listeners are more likely to link male voices with traditional Maya clothing, the traje típico, when their speech includes features of Mayan-accented Spanish. However, as Maya women are more likely than men to wear the traje típico, this matched-guise study investigates native Guatemalans’ perceptions of Mayan-accented Spanish produced by female voices. The results demonstrate that guises with features of Mayan-accented Spanish were more likely to have traje típico as a response than guises without these features. When compared to the previous studies with male-voiced guises, the findings suggest an interaction between gender and Mayan-accented Spanish. Traje típico responses were more common for female-voiced guises than male-voiced guises and occurred at the highest rate among female-voiced guises with features of Mayan-accented Spanish. Thus, gendered and cultural practices are reflected in the indexical fields of Mayan-accented Spanish in Guatemala, regardless of the gender or ethnicity of the listener. That is, the visual body–language link is significantly more essentialized for the identity of a woman than for the identity of a man in Guatemala, suggesting that gendered stereotypes, language ideologies, and embodied practices mutually reinforce one another in the collective consciousness of the region.