Social isolation triggers oxidative status and impairs systemic and hepatic insulin sensitivity in normoglycemic rats

Bové, María; Lama, Adriano; Schiavone, Stefania; Pirozzi, Claudio; Tucci, Paolo; Sikora, Vladyslav; Trinchese, Giovanna; Corso, Gaetano; Morgese, Maria Grazia; Trabace, Luigia

doi:10.1016/j.biopha.2022.112820

Cited by 9 publications

(6 citation statements)

References 85 publications

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“…In addition, the literature data confi rm that social isolation infl uence the occurrence of oxidative stress [21]. Also, Bove et al [7] observed that isolated rats showed signifi cant elevations in ROS and increased concentration of MDA in the liver, which confi rms that social isolation triggers oxidative stress. It is known that the increased lipid peroxidation in isolated rats can be a consequence of the dysfunction of antioxidant enzymes, which confi rms that the antioxidant defense system is insuffi cient to keep the levels of ROS low [7].…”

Section: Discussionmentioning

confidence: 92%

“…Also, Bove et al [7] observed that isolated rats showed signifi cant elevations in ROS and increased concentration of MDA in the liver, which confi rms that social isolation triggers oxidative stress. It is known that the increased lipid peroxidation in isolated rats can be a consequence of the dysfunction of antioxidant enzymes, which confi rms that the antioxidant defense system is insuffi cient to keep the levels of ROS low [7]. Several studies have confi rmed that honey supplementation has therapeutic effects under pathological conditions [22,23].…”

Section: Discussionmentioning

confidence: 94%

“…It is known that psychosocial stress is associated with increased oxidative stress and decreased antioxidant capacity [6]. For example, the increase in hepatic reactive oxygen species (ROS) amount in individually housed rats might be due to social isolation-induced dysfunctions of antioxidant enzymes [7]. Additionally, isolated rats showed a signifi cant increase in malondialdehyde (MDA) [8] which is the end product of lipid peroxidation and biomarker of free radicals.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Status in the Blood of Psychosocially Stressed Rats Treated with Honey

Gavrilović,

Stojiljković,

Stanić

et al. 2023

Acta Veterinaria

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Linden honey represents a unique honey variety valued for its nutritional benefits, distinctive taste and aroma. Phenols, polyphenols, flavonoids, ascorbic acid and phenolic acids in honey have antioxidant activities. This study aimed to investigate the effects of linden honey on the activities of antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx), as well as on the concentration of malondialdehyde (MDA) in individually housed animals. The investigated parameters were quantified using spectrophotometric method for determination of enzyme activities and MDA concentration in the blood. We found that treatment with linden honey in the socially isolated animals significantly increased the enzyme activities of CAT and GPx, and significantly decreased the concentration of MDA. The modulation of CAT and GPx activities in socially isolated animals treated with linden honey may be very important for understanding the role of honey in the capacity of antioxidant defense system to increase and maintain its stability in psychosocial stress conditions. Our results may be important in biomedical research for understanding the role of honey in the amelioration of oxidative stress.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Antioxidant Status in the Blood of Psychosocially Stressed Rats Treated with Honey

Gavrilović,

Stojiljković,

Stanić

et al. 2023

Acta Veterinaria

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“…Determination of 5-HT, 5-hydroxyindolacetic acid (5-HIAA) and KYN levels in colon were performed by using HPLC coupled with an electrochemical detector (Ultimate ECD, Dionex Scientific, Milan, Italy), as previously described ( 24 ). Briefly, colon samples were homogenized and separated by a LC18 reverse phase column (Kinetex, 150 mm × 4.2 mm, ODS 5 μm; Phenomenex, Castel Maggiore-Bologna, Italy).…”

Section: Methodsmentioning

confidence: 99%

Palmitoylethanolamide counteracts high-fat diet-induced gut dysfunction by reprogramming microbiota composition and affecting tryptophan metabolism

Pirozzi,

Coretti,

Opallo

et al. 2023

Front. Nutr.

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Obesity is associated with gastrointestinal (GI) tract and central nervous system (CNS) disorders. High-fat diet (HFD) feeding-induced obesity in mice induces dysbiosis, causing a shift toward bacteria-derived metabolites with detrimental effects on metabolism and inflammation: events often contributing to the onset and progression of both GI and CNS disorders. Palmitoylethanolamide (PEA) is an endogenous lipid mediator with beneficial effects in mouse models of GI and CNS disorders. However, the mechanisms underlining its enteroprotective and neuroprotective effects still need to be fully understood. Here, we aimed to study the effects of PEA on intestinal inflammation and microbiota alterations resulting from lipid overnutrition. Ultramicronized PEA (30 mg/kg/die per os) was administered to HFD-fed mice for 7 weeks starting at the 12th week of HFD regimen. At the termination of the study, the effects of PEA on inflammatory factors and cells, gut microbial features and tryptophan (TRP)-kynurenine metabolism were evaluated. PEA regulates the crosstalk between the host immune system and gut microbiota via rebalancing colonic TRP metabolites. PEA treatment reduced intestinal immune cell recruitment, inflammatory response triggered by HFD feeding, and corticotropin-releasing hormone levels. In particular, PEA modulated HFD-altered TRP metabolism in the colon, rebalancing serotonin (5-HT) turnover and reducing kynurenine levels. These effects were associated with a reshaping of gut microbiota composition through increased butyrate-promoting/producing bacteria, such as Bifidobacterium, Oscillospiraceae and Turicibacter sanguinis, with the latter also described as 5-HT sensor. These data indicate that the rebuilding of gut microbiota following PEA supplementation promotes host 5-HT biosynthesis, which is crucial in regulating intestinal function.

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“…Thus, a mouse aged postnatal day 70 (P70) is approximately equivalent to a human aged 20 years (20). In male rats, social isolation throughout adolescence has been shown to impair hepatic insulin sensitivity without affecting fasted blood-glucose levels (21). Adult C57BL/6J (C57) mice isolated for 8 weeks exhibit increased body fat percentage, without an overall change in body weight (22).…”

Section: Introductionmentioning

confidence: 99%

Involvement of a serotonin/GLP-1 circuit in adolescent isolation-induced diabetes

Kolling

Khan

Balasubramanian

et al. 2023

Preprint

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In 2020, stay-at-home orders were implemented to stem the spread of SARS-CoV-2 worldwide. Social isolation can be particularly harmful to children and adolescents during the pandemic, the prevalence of obesity increased by ~37% in persons aged 2-19. Obesity is often comorbid with type 2 diabetes, which was not assessed in this human pandemic cohort. Here, we investigated whether male mice isolated throughout adolescence develop type 2 diabetes in a manner consistent with human obesity-induced diabetes, and explored neural changes that may underlie such an interaction. We find that isolating C57BL/6J mice throughout adolescence is sufficient to induce type 2 diabetes. We observed fasted hyperglycemia, diminished glucose clearance in response to an insulin tolerance test, decreased insulin signaling in skeletal muscle, decreased insulin staining of pancreatic islets, increased nociception, and diminished plasma cortisol levels compared to group-housed control mice. Using Promethion metabolic phenotyping chambers, we observed dysregulation of sleep and eating behaviors, as well as a time-dependent shift in respiratory exchange ratio of the adolescent-isolation mice. We profiled changes in neural gene transcription from several brain areas and found that a neural circuit between serotonin-producing and GLP-1-producing neurons is affected by this isolation paradigm. Overall, spatial transcription data suggest decreased serotonin neuron activity (via decreased GLP-1-mediated excitation) and increased GLP-1 neuron activity (via decreased serotonin-mediated inhibition). This circuit may represent an intersectional target to further investigate the relationship between social isolation and type 2 diabetes, as well as a pharmacologically-relevant circuit to explore the effects of serotonin and GLP-1 receptor agonists.

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Social isolation triggers oxidative status and impairs systemic and hepatic insulin sensitivity in normoglycemic rats

Cited by 9 publications

References 85 publications

Antioxidant Status in the Blood of Psychosocially Stressed Rats Treated with Honey

Antioxidant Status in the Blood of Psychosocially Stressed Rats Treated with Honey

Palmitoylethanolamide counteracts high-fat diet-induced gut dysfunction by reprogramming microbiota composition and affecting tryptophan metabolism

Involvement of a serotonin/GLP-1 circuit in adolescent isolation-induced diabetes

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