Social Isolation Model: A Noninvasive Rodent Model of Stress and Anxiety

Watanabe, Saki; Omran, Alzahra Al; Shao, Amy S.; Ji, Liang

doi:10.3791/64567

Cited by 3 publications

(2 citation statements)

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“…Animals were isolated postweaning for 3 weeks between P21 and P43 [ 29 , 49 ]. Isolated mice were housed in small (10*10 cm) and opaque cages with limited light access, to visually block the external environment and neighboring mice [ 50 ]. The grouped mice were housed in standard (30*15 cm) cages.…”

Section: Methodsmentioning

confidence: 99%

Postweaning Social Isolation Alters Puberty Onset by Suppressing Electrical Activity of Arcuate Kisspeptin Neurons

Agus,

Yavuz,

Atasoy

et al. 2024

Neuroendocrinology

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Introduction: Postweaning social isolation (PWSI) in rodents is an advanced psychosocial stress model in early life. Some psychosocial stress, such as restrain and isolation, disrupts reproductive physiology in young and adult periods. Mechanisms of early-life stress effects on central regulation of reproduction need to be elucidated. We have investigated the effects of PWSI on function of arcuate kisspeptin (ARCKISS1) neurons by using electrophysiological techniques combining with monitoring of puberty onset and estrous cycle in male and female Kiss1-Cre mice. Methods: Female mice were monitored for puberty onset with vaginal opening examination during social isolation. After isolation, the estrous cycle of female mice was monitored with vaginal cytology. Anxiety-like behavior of mice was determined by an elevated plus maze test. Effects of PWSI on electrophysiology of ARCKISS1 neurons were investigated by the patch clamp method after intracranial injection of AAV-GFP virus into arcuate nucleus of Kiss1-Cre mice after the isolation period. Results: We found that both male and female isolated mice showed anxiety-like behavior. PWSI caused delay in vaginal opening and extension in estrous cycle length. Spontaneous-firing rates of ARCKISS1 neurons were significantly lower in the isolated male and female mice. The peak amplitude of inhibitory postsynaptic currents to ARCKISS1 neurons was higher in the isolated mice, while frequency of excitatory postsynaptic currents was higher in group-housed mice. Conclusion: These findings demonstrate that PWSI alters pre- and postpubertal reproductive physiology through metabolic and electrophysiological pathways.

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Section: Methodsmentioning

confidence: 99%

Postweaning Social Isolation Alters Puberty Onset by Suppressing Electrical Activity of Arcuate Kisspeptin Neurons

Agus,

Yavuz,

Atasoy

et al. 2024

Neuroendocrinology

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show abstract

“…Housing quality and social environment have already been identified as major contributors to cardiovascular health, and although their effect has been documented in rodents (Schmidt et al 2022; Watanabe et al 2022), there are no studies examining how they alter responsiveness to an environmental stressor. For instance, it has already been shown that housing rats in metabolic rather than standard cages result in mild and reversible cardiovascular changes (Giral et al 2022).…”

Section: Introductionmentioning

confidence: 99%

The effect of enriched versus depleted housing on eucalyptus smoke-induced cardiovascular dysfunction in mice

Harmon,

Fiamingo,

Toler

et al. 2024

Preprint

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Living conditions play a major role in health and well-being, particularly for the cardiovascular and pulmonary systems. Depleted housing contributes to impairment and development of disease, but how it impacts body resiliency during exposure to environmental stressors is unknown. This study examined the effect of depleted (DH) versus enriched housing (EH) on cardiopulmonary function and subsequent responses to wildfire smoke. Two cohorts of healthy female mice, one of them surgically implanted with radiotelemeters for the measurement of electrocardiogram, body temperature (Tco) and activity, were housed in either DH or EH for 7 weeks. Telemetered mice were exposed for 1 hour to filtered air (FA) and then flaming eucalyptus wildfire smoke (WS) while untelemetered mice, which were used for ventilatory assessment and tissue collection, were exposed to either FA or WS. Animals were continuously monitored for 5-7 days after exposure. EH prevented a decrease in Tco after radiotelemetry surgery. EH mice also had significantly higher activity levels and lower heart rate during and after FA and WS. Moreover, EH caused a decreased number of cardiac arrhythmias during WS. WS caused ventilatory depression in DH mice but not EH mice. Housing enrichment also upregulated the expression of cardioprotective genes in the heart. The results of this study indicate that housing conditions impact overall health and cardiopulmonary function. More importantly, depleted housing appears to worsen the response to air pollution. Thus, non-chemical factors should be considered when assessing the susceptibility of populations, especially when it comes to extreme environmental events.

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The effect of enriched versus depleted housing on eucalyptus smoke-induced cardiovascular dysfunction in mice

Harmon,

Fiamingo,

Toler

et al. 2024

Inhalation Toxicology

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Social Isolation Model: A Noninvasive Rodent Model of Stress and Anxiety

Cited by 3 publications

References 0 publications

Postweaning Social Isolation Alters Puberty Onset by Suppressing Electrical Activity of Arcuate Kisspeptin Neurons

Postweaning Social Isolation Alters Puberty Onset by Suppressing Electrical Activity of Arcuate Kisspeptin Neurons

The effect of enriched versus depleted housing on eucalyptus smoke-induced cardiovascular dysfunction in mice

The effect of enriched versus depleted housing on eucalyptus smoke-induced cardiovascular dysfunction in mice

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