Social isolation alters ultrasonic vocalizations but not thyroarytenoid neuromuscular junctions in old rats

Johnson, Aaron M.

doi:10.1002/lary.27355

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…Wöhr et al found that male rats exposed to a brief period of social isolation produced more than twice the amount of USVs when compared to other rats, likely due to increased social motivation after isolating [ 5 ]. However, after longer periods of isolation (2–6 months), studies have found that socially isolated male rats produced fewer 50 kHz USVs with lower amplitude in response to a female rat than socially-housed rats [ 155 , 156 ]. Thus, it appears that while short-term social isolation may increase USV production rates in social situations, long-term social isolation decreases the number and amplitude of social USVs.…”

Section: Review Of Sex Differencesmentioning

confidence: 99%

Biological and Acoustic Sex Differences in Rat Ultrasonic Vocalization

et al. 2021

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The rat model is a useful tool for understanding peripheral and central mechanisms of laryngeal biology. Rats produce ultrasonic vocalizations (USVs) that have communicative intent and are altered by experimental conditions such as social environment, stress, diet, drugs, age, and neurological diseases, validating the rat model’s utility for studying communication and related deficits. Sex differences are apparent in both the rat larynx and USV acoustics and are differentially affected by experimental conditions. Therefore, the purpose of this review paper is to highlight the known sex differences in rat USV production, acoustics, and laryngeal biology detailed in the literature across the lifespan.

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Section: Review Of Sex Differencesmentioning

confidence: 99%

Biological and Acoustic Sex Differences in Rat Ultrasonic Vocalization

et al. 2021

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“…1 Observable deficits in SLN function are routinely encountered in the setting of age-related vocal atrophy, iatrogenic injury, vocal fold paralysis, neurodegenerative conditions, and laryngeal synkinesis. 2,3 The underlying pathophysiology of age-related, and neurodegenerative SLN dysfunction is not well understood. 4,5 Nerve conduction studies (NCS) are an important tool in the management and diagnosis of many peripheral nerve pathologies including spinal cord injury, brachial plexus injury, and carpal tunnel syndrome.…”

Section: Introductionmentioning

confidence: 99%

“…Injury to this nerve results in marked functional deficits including diminished laryngeal sensation and loss of pitch control and is associated with an increased frequency of dysphagia, aspiration, and altered voice 1 . Observable deficits in SLN function are routinely encountered in the setting of age‐related vocal atrophy, iatrogenic injury, vocal fold paralysis, neurodegenerative conditions, and laryngeal synkinesis 2,3 …”

Section: Introductionmentioning

confidence: 99%

Neurophysiology of the Superior Laryngeal Nerve in an In Vivo Rat Model

Cvancara,

de Leon,

Baertsch

et al. 2023

The Laryngoscope

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ObjectiveThe superior laryngeal nerve (SLN) is fundamental in laryngeal sensation, cough reflex, and pitch control. SLN injury has substantial consequences including altered sensation, aspiration, and dysphonia. To date, in vivo measurement of the SLN remains elusive. The purpose of this study was to assess the feasibility of recording motor and sensory evoked potentials in a rat SLN model.MethodsTwenty‐two rat hemi‐laryngeal preparations (n = 11) were obtained from 4‐month‐old Sprague–Dawley rats and included in this study. Compound motor action potentials (CMAPs) and motor unit number estimation (MUNE) were calculated by stimulating the SLN at the point of medial extension near the carotid artery and by placing a recording electrode on the cricothyroid muscle. Sensory response was determined through stimulation of the SLN and laryngoscopic visualization of a laryngeal adductor reflex (LAR). SLN and cricothyroid muscle cross‐sections were stained and histologic morphometrics were quantified.ResultsLaryngeal evoked potentials were successfully obtained in all trials. Mean CMAP latency and negative durations were 0.99 ± 0.57 ms and 1.49 ± 0.57 ms, respectively. The median MUNE was 2.06 (IQR 1.88, 3.51). LAR was induced with a mean intensity of 0.69 ± 0.20 mV. Mean axon count, myelin thickness, and g‐ratio were 681 ± 192.2, 1.72 ± 0.26, and 0.45 ± 0.04, respectively.ConclusionsThis study demonstrates the feasibility of recording evoked response potentials following SLN stimulation. We hypothesize that this work will provide a tractable animal model to study changes in laryngeal sensation and cricothyroid motor function with aging, neurodegenerative disease, aspiration, or nerve injury.Level of EvidenceN/A Laryngoscope, 2023

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“…Isolating the animals immediately post-weaning produces symptoms of depression (such as reduced reward anticipation) that do not resolve when the animals are reunited postadolescence (Shetty & Sadananda, 2017). Rats isolated while relatively young have been shown to make fewer Ultra-Sonic Vocalisations (USVs), or show aggressive behaviours towards other rats (Dakendar et al, 2009) and these effects continue into adulthood (Johnson, 2018). There appears to be conflicting evidence about whether anxious/depressive behaviours appear to increase when rats are isolated for a significant period of time.…”

Section: Animal Modelsmentioning

confidence: 99%

Investigating social housing: The effects of isolation on rodent ethanol consumption

Bradley

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Many animal studies investigating Alcohol Use Disorder isolate animals for weeks at a time for accuracy in measures of voluntary consumption. This practice is stressful for the animals, and may be a confound for examining other behaviours following ethanol exposure. For example, chronic isolation and ethanol consumption have been shown to affect anxiety and depression-indicating behaviours in rats, but these studies report ethanol only as affecting anxiety while housing animals individually during ethanol access. Social behaviour has also been shown to moderate ethanol consumption in rats, but this effect has not been tested during the intermittent ethanol access, only in the weeks beforehand. We therefore conducted an experiment applying the IATBCP procedure to rats that were either continuously isolated or pair-housed during ethanol-free periods, split using both male and female rats. The animals had intermittent ethanol access for eight weeks, and then were subsequently tested for anxiety and explorative behaviour using the Successive Alleys Test (SAT). Following this we conducted the Anticipatory Behaviour Paradigm (ABP) to measure the animals’ anticipation to food rewards. The isolated animals showed significantly higher ethanol consumption and preference compared to the paired animals, while the paired animals showed greater anticipation on the ABP. The female rats also consistently consumed significantly more ethanol than the males, and showed greater anticipation. We found no difference in perceived anxiety/exploration using the SAT between any of our three main conditions (housing, sex, drinker/control).These results demonstrate that housing conditions affect both ethanol consumption and anticipatory behaviour in rats, and that paired housing during intermittent ethanol access should be considered by experimenters. Our results also stress the importance of studying both sexes in animal research and the limitations of current animal research procedures.

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Social isolation alters ultrasonic vocalizations but not thyroarytenoid neuromuscular junctions in old rats

Abstract: NA. Laryngoscope, 2018.

Cited by 5 publications

References 28 publications

Biological and Acoustic Sex Differences in Rat Ultrasonic Vocalization

Biological and Acoustic Sex Differences in Rat Ultrasonic Vocalization

Neurophysiology of the Superior Laryngeal Nerve in an In Vivo Rat Model

Investigating social housing: The effects of isolation on rodent ethanol consumption

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